Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chem...Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chemical gradients,and dynamic mechanical forces,of living organs into the microfluidic system,"organs-on-chips"display an unprecedented application potential in a lot of biological fields such as fundamental physiological and pathophysiological research,drug efficacy and toxicity testing,and clinical diagnosis.Here,we review the recent development of organs-on-chips and briefly discuss their future challenges.展开更多
文摘目的:研究慢性偏头痛(chronic migraine,CM)的临床特征,并对国际头痛疾病分类第3版试行版(International Classification of Headache Disorders,3rd Edition,beta version,ICHD-Ⅲbeta)及专家共识版(Expert Opinion criteria,EOc)CM的诊断标准进行临床实践测试。方法:回顾性分析重庆医科大学附属第一医院神经内科头痛门诊2014年6月至2016年6月CM患者的人口学特征及临床特征,并对ICHD-Ⅲbeta及EOc版CM诊断标准进行临床实践测试分析。结果:共纳入236例CM患者,165例(69.9%)患者合并药物过度使用,仅2例(0.8%)患者使用曲普坦类,无患者使用麦角胺类。CM患者的偏头痛亚型中最常见的是无先兆偏头痛(migraine without aura,MO;70.3%),其次为可能性偏头痛(probable migraine,PM;26.3%)和先兆偏头痛(migraine with aura,MA;3.4%)。236例患者均满足EOc版标准,仅167例(70.8%)满足ICHD-Ⅲbeta版CM标准。69例不符合ICHD-Ⅲbeta标准的患者中,62例为不典型偏头痛发作,符合PM,7例为有典型偏头痛发作但既往发作少于5次。结论:CM患者常合并药物过度使用,但偏头痛特异性止痛药曲普坦及麦角胺类使用较少。EOc版CM诊断标准更符合中国CM患者的实际情况,可能更利于在临床中应用。
基金supported by the Ministry of Science and Technology(2012AA022703,2012AA030608,2011CB933201 and 2009CB930001)the National Natural Science Foundation of China(31170905,21025520,51073045,31170905,GZ 614 and 91213305)+1 种基金the Chinese Academy of Sciences(KJCX2-YW-M15)the State Major Scientific and Technological Project of China(2013ZX09507005)
文摘Microfluidic technology provides opportunities to create in vitro models with physiological microenvironment for cell study.Introducing the identified key aspects,including tissue-tissue interfaces,spatiotemporal chemical gradients,and dynamic mechanical forces,of living organs into the microfluidic system,"organs-on-chips"display an unprecedented application potential in a lot of biological fields such as fundamental physiological and pathophysiological research,drug efficacy and toxicity testing,and clinical diagnosis.Here,we review the recent development of organs-on-chips and briefly discuss their future challenges.
