Objective： We investigated the relationship between the expression of Caspase-3, cell proliferation and apoptosis in gastric cancer and their precancerous lesions, to explore the tumorigenesis of the stomach mucosa. ...Objective： We investigated the relationship between the expression of Caspase-3, cell proliferation and apoptosis in gastric cancer and their precancerous lesions, to explore the tumorigenesis of the stomach mucosa. Methods： Caspase- 3 expression in 13 normal gastric mucosa, 6 chronic atrophic gastritis （CAG）, 31 intestinal metaplasia （IM）, 114 dysplasia （DYS） and 20 gastric carcinomas were investigated immunohistochemically. Cell proliferation was evaluated with anti-Ki-67 immunostaining and apoptosis was evaluated using DNA fragmentation in situ by TdT-mediated dUTP biotin nick end labeling （TUNEL） method. Results： Caspase-3 mild-moderately positive expression was observed in most of normal superficial epithelia, its positively polar distribution in normal mucosa, CAG, IM, DYS and gastric carcinomas changed as seen in TU- NEL, and so did the positive rate. Caspase-3 protein expression showed significantly positive correlation with the number of apoptotic cells labeled with TUNEL （correlation coefficient r = 0,94; P 〈 0101）. Ki-67 expression showed a negative but not significant correlation trend with Caspase-3 （correlation coefficient r = -0.23; P 〉 0.05）. Conclusion： Caspase-3 protein expression was up-regulated from CAG to IM and mild-moderate atypical dysplasia, but down-regulated in severe dysplasia and gastric carcinoma, indicating that inactivity or reduced expression of Caspase-3 is closely correlated with carcinogenesis of the stomach mucosa.展开更多
We solve the equilibrium meanfield equation of state of Ising ferromagnet （obtained from Bragg-Williams theory） by Newton-Raphson method. The number of iterations required to get a convergent solution （within a spe...We solve the equilibrium meanfield equation of state of Ising ferromagnet （obtained from Bragg-Williams theory） by Newton-Raphson method. The number of iterations required to get a convergent solution （within a specified accuracy） of equilibrium magnetisation, at any particular temperature, is observed to diverge in a power law fashion as the temperature approaches the critical value. This is identified as the critical slowing down. The exponent is also estimated. This value of the exponent is compared with that obtained from analytic solution. Besides this, the numerical results are also compared with some experimental results exhibiting satisfactory degree of agreement. It is observed from this study that the information of the invariance of time scale at the critical point is present in the meanfield equilibrium equation of state of Ising ferromagnet.展开更多
基金Supported by grants from the National Natural Science Foundation of China (No. 30070845, 30271607, 30600286)Climbing Scholars of the Universities in Liaoning Province (2009–2011)
文摘Objective： We investigated the relationship between the expression of Caspase-3, cell proliferation and apoptosis in gastric cancer and their precancerous lesions, to explore the tumorigenesis of the stomach mucosa. Methods： Caspase- 3 expression in 13 normal gastric mucosa, 6 chronic atrophic gastritis （CAG）, 31 intestinal metaplasia （IM）, 114 dysplasia （DYS） and 20 gastric carcinomas were investigated immunohistochemically. Cell proliferation was evaluated with anti-Ki-67 immunostaining and apoptosis was evaluated using DNA fragmentation in situ by TdT-mediated dUTP biotin nick end labeling （TUNEL） method. Results： Caspase-3 mild-moderately positive expression was observed in most of normal superficial epithelia, its positively polar distribution in normal mucosa, CAG, IM, DYS and gastric carcinomas changed as seen in TU- NEL, and so did the positive rate. Caspase-3 protein expression showed significantly positive correlation with the number of apoptotic cells labeled with TUNEL （correlation coefficient r = 0,94; P 〈 0101）. Ki-67 expression showed a negative but not significant correlation trend with Caspase-3 （correlation coefficient r = -0.23; P 〉 0.05）. Conclusion： Caspase-3 protein expression was up-regulated from CAG to IM and mild-moderate atypical dysplasia, but down-regulated in severe dysplasia and gastric carcinoma, indicating that inactivity or reduced expression of Caspase-3 is closely correlated with carcinogenesis of the stomach mucosa.
文摘We solve the equilibrium meanfield equation of state of Ising ferromagnet （obtained from Bragg-Williams theory） by Newton-Raphson method. The number of iterations required to get a convergent solution （within a specified accuracy） of equilibrium magnetisation, at any particular temperature, is observed to diverge in a power law fashion as the temperature approaches the critical value. This is identified as the critical slowing down. The exponent is also estimated. This value of the exponent is compared with that obtained from analytic solution. Besides this, the numerical results are also compared with some experimental results exhibiting satisfactory degree of agreement. It is observed from this study that the information of the invariance of time scale at the critical point is present in the meanfield equilibrium equation of state of Ising ferromagnet.
