Studies on Quantitative Determination of Active Principles in Chinese Herbal Medicine by Second Derivative Differential Pulse Polarography

The second derivative differential pulse polarography was developed for the quantitative determination of active principles in medicinal herbs (baicalin in Scutellaria baicalensis Georgi, indigo in Baphicacanthus cusia (Nees) Bremek, paeonol in Paeonia suffruticosa Andr and piperine in Piper longum L ) The C O group in baicalin, indigo, paeonol and piperine could be reduced to CH OH in acidic solution at the dropping mercury electrode, and showed very good second derivative differential pulse polarographic peak at 1 550V, 1 300V, 1 630V and 0 860V (vs Ag/AgCl) respectively The linear relationship between concentration of baicalin, indigo, paeonol, piperine and peak height was obtained in the concentration range of 4 5×10 5 ～ 2 7×10 4 mol·L 1 , 3 8×10 5 ～ 4 2×10 4 mol·L 1 , 1 0～6 0×10 4 mol·L 1 and 7 0×10 5 ～2 5×10 4 mol·L 1 and the determination limits were 9 0×10 8 mol·L 1 , 8 4×10 9 mol·L 1 , 9 2×10 9 mol·L 1 and 8 7×10 9 mol·L 1 respectively This method is simple, rapid and sensitive, and the results are accurate

丹皮酚抑制血管平滑肌细胞增殖和对增殖细胞抗原及P27kip1表达的影响

目的 探讨丹皮酚对兔血管成形术后再狭窄的作用及其机制. 方法 选用清洁级雄性新西兰大白兔,采用腹主动脉球囊损伤和高脂饲料喂养方法建立腹主动脉粥样硬化模型,对模型兔腹主动脉狭窄段行2次球囊扩张后分为4组,分别为对照组、低剂量丹皮酚组、高剂量丹皮酚组、雷帕霉素组,分别给予生理盐水2 ml· kg-1·d^-1、低剂量丹皮酚75 mg·kg-1·d^-1、高剂量丹皮酚150mg·kg-1·d^-1、雷帕霉素1.25 mg· kg-1·d^-1灌胃,每天灌胃1次,连续6周给药.6周后取出腹主动脉,使用苏木精-伊红（HE）染色和Masson染色观察狭窄动脉的大体形态学及内膜增生厚度.免疫组化检测增殖细胞抗原（PCNA）表达水平,免疫印迹法（Western-blot）检测P27kip1蛋白表达水平.结果 使用丹皮酚、雷帕霉素后内膜增生程度降低.对照组、低剂量丹皮酚组、高剂量丹皮酚组和雷帕霉素组新生内膜面积分别为0.16±0.03、0.09±0.01、0.05±0.01、0.04±0.01;新生内膜面积/中膜面积分别为90.62±3.43、42.30±2.94、33.72±3.19、28.78±6.67;内膜面积增生率分别为（33.3±3.1）％、（23.2±2.2）％、（17.5±1.5）％、（16.2±1.7）％,差异有统计学意义（F值分别为47.98、257.25、74.60,均P＜0.05）.对照组、低剂量丹皮酚组、高剂量丹皮酚组和雷帕霉素组PCNA的表达水平分别为0.20±0.04、0.06±0.02、0.05±0.02、0.08±0.02,差异均有统计学意义（F=37.22,P＜0.01）;P27kip1表达水平分别为0.47±0.09、0.78±0.20、1.29±0.12、1.83±0.13,差异有统计学意义（F=107.69,P＜0.01）. 结论 丹皮酚可能通过抑制PCNA表达,在细胞周期中上调P27kip1蛋白的表达,起到抗血管平滑肌细胞增殖的作用.