主髂动脉分叉部覆膜支架重建(covered endovascular reconstruction of the aortic bifurcation,CERAB)技术是近些年逐渐推广使用的处理主髂动脉闭塞性病变的腔内治疗手段,与对吻支架技术相比,CERAB技术更加接近主髂动脉分叉处的解剖及...主髂动脉分叉部覆膜支架重建(covered endovascular reconstruction of the aortic bifurcation,CERAB)技术是近些年逐渐推广使用的处理主髂动脉闭塞性病变的腔内治疗手段,与对吻支架技术相比,CERAB技术更加接近主髂动脉分叉处的解剖及血流动力学特点,有利于降低对吻支架植入后血栓形成及内膜增生的发生率,提高远期通畅率。本文报道1例少见的双侧髂动脉闭塞性病变合并腹主动脉多发穿透性主动脉溃疡(penetrating aortic ulcer,PAU)患者的病历资料,在通过采用CERAB技术成功恢复双髂动脉血运的同时隔绝了腹主动脉多发PAU,改善了患者腹痛及下肢缺血的症状。展开更多
[Objective] This study aimed to develop an indirect ELISA to detect the antibodies against Actinobacil us pleuropneumoniae （APP） using the recombinant ApxⅡA1 protein expressed in prokaryotic cells as the antigen. [...[Objective] This study aimed to develop an indirect ELISA to detect the antibodies against Actinobacil us pleuropneumoniae （APP） using the recombinant ApxⅡA1 protein expressed in prokaryotic cells as the antigen. [Method] The major epi-tope domain of ApxⅡ was cloned into the prokaryotic expression vector pET-28a （＋） to obtain the recombinant plasmid pET-ApxⅡA1, which was then transformed into E. coli BL21 （DE3） for expression. The immunogenicity of the recombinant pro-tein was analyzed by western-blotting. After that, the purified recombinant protein was used as the coating antigen in the indirect ELISA for detecting the antibodies against APP. Final y, the concentration of coated antigen and the dilution of serum were optimized. [Result] Proved by enzyme digestion and sequencing, the recombi-nant plasmid pET-ApxⅡA1 was constructed successful y. The recombinant protein was highly expressed in prokaryotic cells, and Western-blotting analysis showed that it was recognized specifical y by positive serum of APP. The indirect ELISA could detect the antibody against APP with the purified recombinant protein as the coating antigen. The optimal concentration of coated antigen was 1.23 μg/ml and the opti-mal dilution of serum was 1：100. Compared with a commercial ELISA kit detecting antibody against ApxⅣ, the coincidence rate of the indirect ELISA was 90.4%. [Conclusion] Our results indicated that the indirect ELISA is sensitive and specific, and suitable for evaluating the effect of APP vaccine and epidemiological surveys.展开更多
SILENT extra-adrenal retroperitoneal paragangliomas (PGLs) arise from dispersed paraganglias which tend to be symmetrically distributed in close relation to the aorta and sympatheticnervous system. They are rarely enc...SILENT extra-adrenal retroperitoneal paragangliomas (PGLs) arise from dispersed paraganglias which tend to be symmetrically distributed in close relation to the aorta and sympatheticnervous system. They are rarely encountered in everyday surgical practice.展开更多
文摘主髂动脉分叉部覆膜支架重建(covered endovascular reconstruction of the aortic bifurcation,CERAB)技术是近些年逐渐推广使用的处理主髂动脉闭塞性病变的腔内治疗手段,与对吻支架技术相比,CERAB技术更加接近主髂动脉分叉处的解剖及血流动力学特点,有利于降低对吻支架植入后血栓形成及内膜增生的发生率,提高远期通畅率。本文报道1例少见的双侧髂动脉闭塞性病变合并腹主动脉多发穿透性主动脉溃疡(penetrating aortic ulcer,PAU)患者的病历资料,在通过采用CERAB技术成功恢复双髂动脉血运的同时隔绝了腹主动脉多发PAU,改善了患者腹痛及下肢缺血的症状。
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303034-8)~~
文摘[Objective] This study aimed to develop an indirect ELISA to detect the antibodies against Actinobacil us pleuropneumoniae （APP） using the recombinant ApxⅡA1 protein expressed in prokaryotic cells as the antigen. [Method] The major epi-tope domain of ApxⅡ was cloned into the prokaryotic expression vector pET-28a （＋） to obtain the recombinant plasmid pET-ApxⅡA1, which was then transformed into E. coli BL21 （DE3） for expression. The immunogenicity of the recombinant pro-tein was analyzed by western-blotting. After that, the purified recombinant protein was used as the coating antigen in the indirect ELISA for detecting the antibodies against APP. Final y, the concentration of coated antigen and the dilution of serum were optimized. [Result] Proved by enzyme digestion and sequencing, the recombi-nant plasmid pET-ApxⅡA1 was constructed successful y. The recombinant protein was highly expressed in prokaryotic cells, and Western-blotting analysis showed that it was recognized specifical y by positive serum of APP. The indirect ELISA could detect the antibody against APP with the purified recombinant protein as the coating antigen. The optimal concentration of coated antigen was 1.23 μg/ml and the opti-mal dilution of serum was 1：100. Compared with a commercial ELISA kit detecting antibody against ApxⅣ, the coincidence rate of the indirect ELISA was 90.4%. [Conclusion] Our results indicated that the indirect ELISA is sensitive and specific, and suitable for evaluating the effect of APP vaccine and epidemiological surveys.
文摘SILENT extra-adrenal retroperitoneal paragangliomas (PGLs) arise from dispersed paraganglias which tend to be symmetrically distributed in close relation to the aorta and sympatheticnervous system. They are rarely encountered in everyday surgical practice.
