目的调查川南地区乙型肝炎病毒(hepatitis B virus,HBV)基因型的分布,探讨其临床价值。方法采用PCR-反向点杂交法对172例HBV病毒载量≥1.00×104IU的乙肝患者进行HBV基因分型,了解基因型在不同临床类型肝病中的分布及其与患者肝功能...目的调查川南地区乙型肝炎病毒(hepatitis B virus,HBV)基因型的分布,探讨其临床价值。方法采用PCR-反向点杂交法对172例HBV病毒载量≥1.00×104IU的乙肝患者进行HBV基因分型,了解基因型在不同临床类型肝病中的分布及其与患者肝功能、HBV病毒载量、年龄、性别之间的关系。结果①共检测了172例患者,成功分型166例,占96.51%。其中B型98例,占56.98%;C型60例,占34.88%;B/C混合型8例,占4.65%;未知型6例,占3.49%;②B型多见于无症状乙肝病毒携带者(ASC,占59.18%);在急、慢性肝炎(AHB、CHB)、肝硬化患者(HC)、肝细胞癌(HCC)等疾病中,C基因型比例明显高于ASC(P<0.05),但几者之间差异无显著性(P>0.05);③C基因型患者年龄高于B基因型患者(P<0.05);C基因型患者肝功能指标ALT、TBiL均高于B基因型患者(P<0.05),但GGT、HBV载量、性别比差异无显著性(P>0.05)。结论川南地区乙型肝炎病毒基因型以B型为主,C型次之,B型和C型共占91.86%,其他型别偶见;C基因型可能与乙肝患者肝功能损害和严重肝脏疾病的发生有关而与患者性别、HBV病毒载量高低无关。展开更多
Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and cli...Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and clinical outcome of HBV genotypes and genotype mixtures in HBV-infected patients from Vietnam,Europe,and Africa.In addition,we analyzed the effects of genotype mixtures on alterations in in vitro viral replication.In Asian patients,seven genotypes(A-G) were detected,with A,C,and D predominating.In European and African patients,only genotypes A,C,D,and G were identified.Genotype mixtures were more frequently encountered in African than in Asian(P =.01) and European patients(P =.06) .In Asian patients,the predominant genotype mixtures included A/C and C/D,compared to C/D in European and A/D in African patients.Genotype A was more frequent in asymptomatic compared with symptomatic patients(P <.0001) .Genotype C was more frequent in patients with hepatocellular carcinoma(HCC;P =.02) .Genotype mixtures were more frequently encountered in patients with chronic hepatitis in comparison to patients with acute hepatitis B(P =.015) ,liver cirrhosis(P =.013) ,and HCC(P =.002) .Viral loads in patients infected with genotype mixtures were significantly higher in comparison to patients with a single genotype(P =.019) .Genotype mixtures were also associated with increased in vitro HBV replication.In conclusion,infection with mixtures of HBV genotypes is frequent in Asia,Africa,and Europe.Differences in the replication-phenotype of single genotypes compared to genotype-mixtures suggest that co-infection with different HBV-genotypes is associated with altered pathogenesis and clinical outcome.展开更多
Background/Aims:We analyzed the characteristics of HIV infected patients who died from liver disease, focusing on hepatitis virus co-infection. Methods:One-hundred and eighty-five French hospital departments involved ...Background/Aims:We analyzed the characteristics of HIV infected patients who died from liver disease, focusing on hepatitis virus co-infection. Methods:One-hundred and eighty-five French hospital departments involved in HIV/AIDS management prospectively notified all deaths occurring in 2000. Patients whose hepatitis C (HCV) and hepatitis B (HBV) serostatus was known were classified as being infected by HCV alone, HBV alone (HBsAg positive), both HCV and HBV, or neither HCV nor HBV. Results:Among 822 HIV infected patients, 29%were infected by HCV alone, 8%by HBV alone, and 4%by both HCV and HBV. The most frequent causes of death were liver disease (31%of cases) and AIDS (29%) among HIV-HCV co-infected patients, and AIDS (38%) and liver disease (22%) among HIV-HBV co-infected patients. Liver disease was a more frequent cause of death among patients co-infected by both HCV and HBV (44%of cases). Hepatocellular carcinoma was present in 15%of patients who died from liver disease, and was associated with HBV co-infection. Nearly half the patients who died from liver disease had more than 200 CD4/mm3. Conclusions:Liver disease is now a leading cause of death among HIV-HCV co-infected patients and is becoming an important cause of death among HIV-HBV co-infected patients. The risk of death from liver disease is highest in patients co-infected by both HCV and HBV.展开更多
文摘目的调查川南地区乙型肝炎病毒(hepatitis B virus,HBV)基因型的分布,探讨其临床价值。方法采用PCR-反向点杂交法对172例HBV病毒载量≥1.00×104IU的乙肝患者进行HBV基因分型,了解基因型在不同临床类型肝病中的分布及其与患者肝功能、HBV病毒载量、年龄、性别之间的关系。结果①共检测了172例患者,成功分型166例,占96.51%。其中B型98例,占56.98%;C型60例,占34.88%;B/C混合型8例,占4.65%;未知型6例,占3.49%;②B型多见于无症状乙肝病毒携带者(ASC,占59.18%);在急、慢性肝炎(AHB、CHB)、肝硬化患者(HC)、肝细胞癌(HCC)等疾病中,C基因型比例明显高于ASC(P<0.05),但几者之间差异无显著性(P>0.05);③C基因型患者年龄高于B基因型患者(P<0.05);C基因型患者肝功能指标ALT、TBiL均高于B基因型患者(P<0.05),但GGT、HBV载量、性别比差异无显著性(P>0.05)。结论川南地区乙型肝炎病毒基因型以B型为主,C型次之,B型和C型共占91.86%,其他型别偶见;C基因型可能与乙肝患者肝功能损害和严重肝脏疾病的发生有关而与患者性别、HBV病毒载量高低无关。
文摘Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and clinical outcome of HBV genotypes and genotype mixtures in HBV-infected patients from Vietnam,Europe,and Africa.In addition,we analyzed the effects of genotype mixtures on alterations in in vitro viral replication.In Asian patients,seven genotypes(A-G) were detected,with A,C,and D predominating.In European and African patients,only genotypes A,C,D,and G were identified.Genotype mixtures were more frequently encountered in African than in Asian(P =.01) and European patients(P =.06) .In Asian patients,the predominant genotype mixtures included A/C and C/D,compared to C/D in European and A/D in African patients.Genotype A was more frequent in asymptomatic compared with symptomatic patients(P <.0001) .Genotype C was more frequent in patients with hepatocellular carcinoma(HCC;P =.02) .Genotype mixtures were more frequently encountered in patients with chronic hepatitis in comparison to patients with acute hepatitis B(P =.015) ,liver cirrhosis(P =.013) ,and HCC(P =.002) .Viral loads in patients infected with genotype mixtures were significantly higher in comparison to patients with a single genotype(P =.019) .Genotype mixtures were also associated with increased in vitro HBV replication.In conclusion,infection with mixtures of HBV genotypes is frequent in Asia,Africa,and Europe.Differences in the replication-phenotype of single genotypes compared to genotype-mixtures suggest that co-infection with different HBV-genotypes is associated with altered pathogenesis and clinical outcome.
文摘Background/Aims:We analyzed the characteristics of HIV infected patients who died from liver disease, focusing on hepatitis virus co-infection. Methods:One-hundred and eighty-five French hospital departments involved in HIV/AIDS management prospectively notified all deaths occurring in 2000. Patients whose hepatitis C (HCV) and hepatitis B (HBV) serostatus was known were classified as being infected by HCV alone, HBV alone (HBsAg positive), both HCV and HBV, or neither HCV nor HBV. Results:Among 822 HIV infected patients, 29%were infected by HCV alone, 8%by HBV alone, and 4%by both HCV and HBV. The most frequent causes of death were liver disease (31%of cases) and AIDS (29%) among HIV-HCV co-infected patients, and AIDS (38%) and liver disease (22%) among HIV-HBV co-infected patients. Liver disease was a more frequent cause of death among patients co-infected by both HCV and HBV (44%of cases). Hepatocellular carcinoma was present in 15%of patients who died from liver disease, and was associated with HBV co-infection. Nearly half the patients who died from liver disease had more than 200 CD4/mm3. Conclusions:Liver disease is now a leading cause of death among HIV-HCV co-infected patients and is becoming an important cause of death among HIV-HBV co-infected patients. The risk of death from liver disease is highest in patients co-infected by both HCV and HBV.
