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一种B型CpG ODN对乙肝疫苗的免疫佐剂作用 被引量:2
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作者 董博翰 王莉 +3 位作者 卫红飞 张培因 于永利 王丽颖 《免疫学杂志》 CAS CSCD 北大核心 2009年第1期52-55,共4页
目的研究一种本室设计的B型CpG ODN(BW006)作为乙肝疫苗佐剂的可行性。方法以琼脂糖凝胶电泳法对BW006的质量进行初步检定;H3-掺入法检测BW006对小鼠脾细胞的刺激作用;乙肝疫苗与BW006联合免疫Balb/c小鼠,并用ELISA法检测小鼠血清中的HB... 目的研究一种本室设计的B型CpG ODN(BW006)作为乙肝疫苗佐剂的可行性。方法以琼脂糖凝胶电泳法对BW006的质量进行初步检定;H3-掺入法检测BW006对小鼠脾细胞的刺激作用;乙肝疫苗与BW006联合免疫Balb/c小鼠,并用ELISA法检测小鼠血清中的HBsAb水平。结果BW006的纯度和完整性符合实验要求,并能显著的刺激小鼠脾细胞的增生,同乙肝疫苗联用后能够明显增强乙肝疫苗的免疫效果。结论BW006对乙肝疫苗有较强的免疫佐剂作用。 展开更多
关键词 CPG ODN 乙型肝炎肝疫苗 佐剂 HBSAG
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New therapeutic vaccination strategies for the treatment of chronic hepatitis B 被引量:9
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作者 Jia Liu Anna Kosinska +1 位作者 Mengji Lu Michael Roggendorf 《Virologica Sinica》 SCIE CAS CSCD 2014年第1期10-16,共7页
Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in o... Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in only about 30%patients and is associated with side effects.Most patients receiving nucleot(s)ide analogue treatment do not establish long-term,durable control of Infection and have rebounding viremia after cessation of therapy.Thus,novel therapy strategies are necessary to achieve the induction of potent and durable antiviral immune responses of the patients which can maintain long-term control of viral replication.Therapeutic vaccination of HBV carriers is a promising strategy for the control of hepatitis B.Here the authors review new therapeutic vaccination strategies to treat chronic hepatitis B which may be introduced for patient treatment in the future. 展开更多
关键词 hepatitis B virus woodchuck hepatitis virus therapeutic vaccination IMMUNOMODULATION programmed death-1
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Four-year follow up of hepatitis C patients vaccinated against hepatitis B virus
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作者 Slawomir Chlabicz Tadeusz Wojciech Lapinski +1 位作者 Anna Grzeszczuk Danuta Prokopowicz 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第12期1798-1801,共4页
AIM: Patients with chronic hepatitis C have been recommended to receive vaccinations against hepatitis B. Our study aimed at evaluating the hepatitis B immunogenicity and efficacy against hepatitis B virus infection 4... AIM: Patients with chronic hepatitis C have been recommended to receive vaccinations against hepatitis B. Our study aimed at evaluating the hepatitis B immunogenicity and efficacy against hepatitis B virus infection 4 years after primary immunization series in a group of patients with chronic hepatitis C.METHODS: We recruited 36 out of 48 hepatitis C virus (HCV) infected individuals who were vaccinated against hepatitis B virus (20 μg of recombinant HBsAg at 0-1-6mo schedule) in 1998. Here we measured anti-HBs titers and anti-HBc 4 years after delivery of the third dose of primary immunization series.RESULTS: After 4 years a total of 13/36 (36%) HCV infected patients had seroprotective titers of anti-HBs compared with 9/10 (90%) in the control group, (P<0.05).Similarly the mean concentration of anti-HBs found in hepatitis C patients was significantly lower than that found in healthy subjects (18.3 and 156.0 mIU/mL respectively (P<0.05). None of the HCV infected patients or controls became infected with HBV during the study period as confirmed by anti-HBc negativity.CONCLUSION: We demonstrated that 4 years after HBV immunizations' more than 60% of vaccinated HCV patients did not maintain seroprotective levels of anti-HBs, which might put them at risk of clinically significant breakthrough infections. Further follow-up studies are required to clarify whether memory B and T lymphocytes can provideprotection in chronic hepatitis C patients in the absence or inadequate titers of anti-HBs. 展开更多
关键词 Hepatitis B Hepatitic C vaccine Follw-up studies
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Hepatitis B virus S gene mutants in infants infected despite immunoprophylaxis 被引量:4
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作者 朱启镕 吕晴 +2 位作者 俞蕙 段恕诚 熊思东 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第4期16-18,102,共4页
Objective To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure Methods Using sera from 106 infants who were born to HBV carrier mothers a... Objective To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure Methods Using sera from 106 infants who were born to HBV carrier mothers and failed in HB immunoprophylaxis, HBV S gene was amplified by PCR, transferred to nylon membranes for Southern blots, and then hybridized with oligonucleotide probes Eleven of non hybridizing samples were used for DNA sequencing Results 93 4% (99/106) of the samples were HBV DNA positive, and 30 3% (30/99) failed to hybridize with at least one of the four probes DNA sequencing confirmed that 10 of the 11 samples had an S gene mutation with amino acid (aa) change The identified mutants included nucleotide (nt) 546T→A(aa131N→T), nt531T→C (aa126I→T), nt491A→C (aa113T→P), nt491T→A (aa113S→T), nt533C→A (aa127P→T), nt581T→A (aa143S→T), nt636A→T (aa161Y→F), and nt679A→C (aa175L→F) The sequence in one mother infant pair was completely the same, with mutations at aa131 and aa161 Conclusions The prevalence of HBV surface mutants is about 30% in the children failing in HB vaccination HBV mutants can infect infants by maternal infant transmission 展开更多
关键词 hepatitis B virus · S gene mutant · hepatitis B vaccine
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