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番茄乙烯形成酶基因的克隆及其反义基因的表达载体构建 被引量:1
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作者 李广存 毕玉平 +4 位作者 李杰文 单雷 王秀丽 路艳辉 徐平丽 《山东农业科学》 1999年第3期8-11,共4页
从成熟的番茄果实中提取总RNA,进行RT-PCR扩增合成乙烯形成酶cDNA,并将其克隆至载体Bluescript的EcoRV位点,经限制酶谱分析,构建亚克隆进行全序列测定和序列分析。将所克隆基因以反义基因的形式定向重... 从成熟的番茄果实中提取总RNA,进行RT-PCR扩增合成乙烯形成酶cDNA,并将其克隆至载体Bluescript的EcoRV位点,经限制酶谱分析,构建亚克隆进行全序列测定和序列分析。将所克隆基因以反义基因的形式定向重组到载体pSPROK上,同时将带有完整启动子和终止子的标记基因BAR基因引入pSPROK中,最终获得表达乙烯形成酶反义基因和BAR基因的植物表达载体pBAR-EFE。 展开更多
关键词 EFE基因 BAR基因 表达载体 番茄乙烯形成
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Formability of AA5052/polyethylene/AA5052 sandwich sheets 被引量:3
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作者 刘建光 薛卫 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第4期964-969,共6页
The formability of AA5052/polyethylene/AA5052 sandwich sheets was experimentally studied. Three kinds of AA5052/polyethylene/AA5052 sandwich specimens with different thicknesses of core materials were prepared by the ... The formability of AA5052/polyethylene/AA5052 sandwich sheets was experimentally studied. Three kinds of AA5052/polyethylene/AA5052 sandwich specimens with different thicknesses of core materials were prepared by the hot pressing adhesive method. Then, the uniaxial tensile tests were conducted to investigate the mechanical properties of AA5052/polyethylene/ AA5052 sandwich sheets, and the stretching tests were carried out to investigate the influences of polymer core thickness on the limit dome height of the sandwich sheet. The forming limit curves for three kinds of sandwich sheets were obtained. The experimental results show that the forming limit of the AA5052/polyethylene/AA5052 sandwich sheet is higher than that of the monolithic AA5052 sheet, and it increases with increasing the thickness of polyethylene core. 展开更多
关键词 AA5052 aluminum alloy sandwich sheets POLYETHYLENE FORMABILITY forming limit diagram
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怎样对西瓜进行人工催熟和增甜
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作者 吴国兴 《农村实用科技信息》 1995年第8期9-9,共1页
西瓜果实成熟的内源激素是乙烯利。在果实内乙烯利的增长受生长素的拮抗,生长素越多,乙烯形成越少。随着果实生长的停止和生长素的减少,乙烯利的含量逐渐增加,乙烯利形成的愈早,成熟过程完成越快。把未充分成熟的果实放在具有少量乙烯... 西瓜果实成熟的内源激素是乙烯利。在果实内乙烯利的增长受生长素的拮抗,生长素越多,乙烯形成越少。随着果实生长的停止和生长素的减少,乙烯利的含量逐渐增加,乙烯利形成的愈早,成熟过程完成越快。把未充分成熟的果实放在具有少量乙烯利的环境(1:20000)能刺激成熟过程。试验证明。 展开更多
关键词 乙烯 西瓜 生长素 果实成熟 内源激素 果实生长 成熟过程 增甜 乙烯形成 含糖量
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建议开展基因工程防治松材线虫病的研究
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作者 周学尚 《安徽林业科技》 1998年第5期24-24,共页
关键词 工程防治 线虫病 松材线虫 基因导入 基因工程 乙烯形成 木质部栓塞 森林病虫害 基因操作 酶基因
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Eutectic effect during mesophase formation in co-carbonization of ethylene tar pitch and polystyrene 被引量:2
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作者 Cheng Xianglin Song Shulei 《International Journal of Mining Science and Technology》 2012年第2期183-186,共4页
Ethylene tar pitch was co-carbonized with waste polystyrene to prepare mesophase pitch. The character- istics of mesophase pitches were examined using polarized light optical microscopy, apparent viscome- try, Fourier... Ethylene tar pitch was co-carbonized with waste polystyrene to prepare mesophase pitch. The character- istics of mesophase pitches were examined using polarized light optical microscopy, apparent viscome- try, Fourier transform infrared spectrometry, IH nuclear magnetic resonance spectrometry, and X-ray diffractometry. The properties of the mesophase pitch were greatly improved because of the eutectic effect. The soluble content increased from 5% to 56%, the mesophase itself increased from 32% to 100%, and the optical texture was changed from a coarse mosaic into a flow domain after the waste polystyrene was added to the ethylene tar pitch. The apparent viscosity showed that the mesophase pitch changed from thixotropic to Newtonian suggesting improved rheological behavior during co-carbonization)The increased number of alkyl groups, which are mainly methylene groups, altered the molecular structure of the mesophase pitch in a way that resulted in the eutectic effect. 展开更多
关键词 Eutectic effectEthylene tar pitchWaste polystyreneCo-carbonizationMesophase pitch
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The Research of Complex System Property between Thermosensitive Polymer and Surfactant
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作者 Chao Ma Gang Wan Tang Tang 《Journal of Energy and Power Engineering》 2015年第8期721-726,共6页
In this paper, the rigid structural thermosensitive polymer (made in lab) of NBS (N-butyl styrene), N, N-DEAM (diethyl acrylamide) and AM (acrylamide) was prepared. The influence of viscosity for copolymer sol... In this paper, the rigid structural thermosensitive polymer (made in lab) of NBS (N-butyl styrene), N, N-DEAM (diethyl acrylamide) and AM (acrylamide) was prepared. The influence of viscosity for copolymer solution under different reaction conditions such as temperatures and inorganic salt (monovalent salt and divalent salt) was analyzed. The experiment studies the combination of polymer situation and three different types of surfactants under certain conditions of the room temperature (25℃) and the formation temperature (76℃). At last, the influence of the surfactant kinds and concentration on the viscosity of the polymer solution are studied. The results show that: The copolymer solution, the apparent viscosity of which decreases with the increasement of temperature, but its viscosity has suddenly increased and thereafter dropped in the transition temperature. So the temperature sensitive effect ofcopolymer is very significantly. When the concentration of inorganic salt and surfactant can be controlled in certain extent, the copolymer solution, the effect increases with the increasement of the concentration, but the viscosity of which decreases with the increasement of shear rate. Shear rate can be controlled in certain extent, shearing stability properties of the copolymer solution are proved. 展开更多
关键词 Butyl styrene hydrophobic monomer solution properties thermosensitive polymer surfactant.
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国外农业新技术
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作者 兰军 《新农村》 1997年第10期25-25,共1页
1.新型除草剂最近,美国研究成功一种新型除草剂,这种除草剂喷洒到野草等植物表面,能使植物的合成通道发生亢进,从而导致野草迅速死亡,但对大麦、小麦和燕麦等庄稼都毫无影响。据报道,这种除草剂会有选择地破坏植物的细胞,杀死藜、芥和... 1.新型除草剂最近,美国研究成功一种新型除草剂,这种除草剂喷洒到野草等植物表面,能使植物的合成通道发生亢进,从而导致野草迅速死亡,但对大麦、小麦和燕麦等庄稼都毫无影响。据报道,这种除草剂会有选择地破坏植物的细胞,杀死藜、芥和红根苋之类的野草。2.生物工程番茄最近,美国培育出一种生物工程番茄,其主要特点是味道鲜美,长在植株上不变软、不破损,长期存放不腐烂。一般番茄在成熟时会形成一种催熟荷尔蒙—乙烯。为了防止腐烂,往往在番茄绿色、坚硬时,即乙烯形成前进行采摘。生物工程番茄利用基因重组技术,抑制乙烯的产生。 展开更多
关键词 生物工程 新型除草剂 番茄 基因重组技术 美国研究 机器人 植物表面 乙烯形成 主要特点 长期存放
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Short-term survival analysis of the all-polyethylene tibial component in total knee arthroplasty
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作者 沈彬 杨静 裴福兴 《Chinese Journal of Traumatology》 CAS 2005年第2期126-128,共3页
Objective: To report the clinical and radiological results of 24 total knee arthroplasty in which all-polyethylene tibial components were used. Methods: Between December 2000 and December 2002, 24 cemented total k... Objective: To report the clinical and radiological results of 24 total knee arthroplasty in which all-polyethylene tibial components were used. Methods: Between December 2000 and December 2002, 24 cemented total knee arthroplasty in 21 patients were performed using all-polyethylene tibial components. The mean age of the 21 patients (9 men and 12 women) at operation was 55 years, ranging 48-61 years. The mean preoperative hospital for special surgery (HSS)score was 40.2 (range, 36-43). The diagnoses were degenerative osteoarthritis in 15 patients, rheumatoid arthritis in 5 and traumatic arthritis in 1. All the operations were performed by the same surgeon group and there were unilateral operations in 18 patients and bilateral operations in 3. Results: Eighteen patients were followed up with a follow-up rate of 85.7%. The mean follow-up is 2.5 years (range, 1-3 years) and mean postoperative HSS scores was 87.5 (range, 83-89). Among them, 18 were excellent, 3 good, 3 poor and none was fair (the results of three lost patients were classified as poor). Of those reviewed, the prosthesis was all in situ and no revision occurred. Radiological assessment of these patients revealed 4 ( 16.67%) tibial components with radiolucent lines (mean width≤2 mm) distributed mainly in zone 1 and zone 4. None of these knees was symptomatic. Conclusions: The result of total knee arthroplasty using all-polyethylene tibial components is encouraging. The operative techniques are similar to those in arthroplasty using metal-backed tibial component. 展开更多
关键词 Arthroplsty replacement knee POLYETHYLENE Prostheses and implants FOLLOW-UP
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