Glutaminase is used industrially to enhance flavor and aroma and to enrich foodstuffs nutritionally. It also has potential for pharmaceutical application as anti-leukemia agent. The bacteria of Zymomonas mobilis has b...Glutaminase is used industrially to enhance flavor and aroma and to enrich foodstuffs nutritionally. It also has potential for pharmaceutical application as anti-leukemia agent. The bacteria of Zymomonas mobilis has been studied for ethanol production, however, there is no study regarding glutaminase production. The aim of the present study was to establish the influencing factors for the growth of Z mobilis and its relationship with glutaminase production using statistical tools. Analysis of variance in blocks was carried out in a complete block design and the Tukey test demonstrated the importance of the components of the culture medium, absence of agitation and fermentation time. Minimum culture medium was used in the optimization varying the glucose concentrations （10, 30, and 50 g/L）, glutamine （0, 0.5 and 1g/L） and culture time （18, 24 and 30 hours）. Maximum production was obtained at 8.86 U/L glutaminase. Optimized conditions were used in the growth kinetics, where typical exponential growth was observed. Glutaminase production was shown to be related to biomass production.展开更多
文摘Glutaminase is used industrially to enhance flavor and aroma and to enrich foodstuffs nutritionally. It also has potential for pharmaceutical application as anti-leukemia agent. The bacteria of Zymomonas mobilis has been studied for ethanol production, however, there is no study regarding glutaminase production. The aim of the present study was to establish the influencing factors for the growth of Z mobilis and its relationship with glutaminase production using statistical tools. Analysis of variance in blocks was carried out in a complete block design and the Tukey test demonstrated the importance of the components of the culture medium, absence of agitation and fermentation time. Minimum culture medium was used in the optimization varying the glucose concentrations （10, 30, and 50 g/L）, glutamine （0, 0.5 and 1g/L） and culture time （18, 24 and 30 hours）. Maximum production was obtained at 8.86 U/L glutaminase. Optimized conditions were used in the growth kinetics, where typical exponential growth was observed. Glutaminase production was shown to be related to biomass production.
