目的探讨细菌性阴道病(BV)与外阴阴道假丝酵母菌病(VVC)患者阴道乳杆菌的功能状态及治疗后阴道乳杆菌的重建情况,为BV与VVC的乳杆菌补充治疗提供依据。方法选取BV与VVC患者及正常对照组女性各30例,采用前后对照、标准对照的方法,通过检...目的探讨细菌性阴道病(BV)与外阴阴道假丝酵母菌病(VVC)患者阴道乳杆菌的功能状态及治疗后阴道乳杆菌的重建情况,为BV与VVC的乳杆菌补充治疗提供依据。方法选取BV与VVC患者及正常对照组女性各30例,采用前后对照、标准对照的方法,通过检测患者治疗前后阴道乳杆菌功能(定植密度、乳杆菌分级、产酸能力、产H;O;能力、体外增殖能力)研究该阴道炎症状态下阴道乳杆菌功能的变化和重建情况。结果BV患者阴道乳杆菌定植密度为(5.87±1.83)个/HP,阴道乳杆菌分级正常比例仅为6.67%,H;O;阳性率53.33%,24 h pH值为5.45,24 h菌液浊度为23.7 MCF。BV患者治愈后阴道乳杆菌定植密度为(45.28±15.05)个/HP,乳杆菌分级正常比例为54.55%,H;O;阳性率81.82%,24 h pH值为4.49,24 h菌液浊度为46.6 MCF。VVC患者阴道乳杆菌定植密度为(48.53±10.59)个/HP,乳杆菌分级正常比例为73.33%,H;O;阳性率为80.00%,24 h pH值为4.41,24 h菌液浊度为52.2 MCF。VVC患者治愈后阴道乳杆菌定植密度为(47.95±10.28)个/HP,乳杆菌分级正常比例为90.48%,H;O;阳性率85.71%,24 h pH值为4.33,24 h菌液浊度为51.3 MCF。结论BV患者阴道乳杆菌功能减退,治愈后未能完全重建,需帮助恢复阴道乳杆菌功能状态。VVC患者阴道乳杆菌功能基本正常。展开更多
AIM:To investigate the mechanisms of Lactobacillus plantarum(L.plantarum)action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into ...AIM:To investigate the mechanisms of Lactobacillus plantarum(L.plantarum)action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into groups of sham-operation,bile duct ligation(BDL),BDL +L.plantarum,BDL+internal biliary drainage(IBD),and BDL+IBD+L.plantarum.Ten days after L.plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C(PKC)activity measurement.The distribution and expression of the PKC and tight junction(TJ)proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS:L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase(BDL vs BDL+L.plantarum,0.295±0.007 vs 0.349±0.003,P<0.05;BDL+IBD vs BDL+IBD+L.plantarum,0.407±0.046 vs 0.465±0.135,P<0.05),and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice(BDL vs BDL+L.plantarum,0.266±0.118 vs 0.326±0.009,P<0.05).The protective effect of L.plantarum was more prominent after internal biliary drainage(BDL+IBD vs BDL +IBD+L.plantarum,0.415±0.105 vs 0.494±0.145,P<0.05).CONCLUSION:L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.展开更多
文摘目的探讨细菌性阴道病(BV)与外阴阴道假丝酵母菌病(VVC)患者阴道乳杆菌的功能状态及治疗后阴道乳杆菌的重建情况,为BV与VVC的乳杆菌补充治疗提供依据。方法选取BV与VVC患者及正常对照组女性各30例,采用前后对照、标准对照的方法,通过检测患者治疗前后阴道乳杆菌功能(定植密度、乳杆菌分级、产酸能力、产H;O;能力、体外增殖能力)研究该阴道炎症状态下阴道乳杆菌功能的变化和重建情况。结果BV患者阴道乳杆菌定植密度为(5.87±1.83)个/HP,阴道乳杆菌分级正常比例仅为6.67%,H;O;阳性率53.33%,24 h pH值为5.45,24 h菌液浊度为23.7 MCF。BV患者治愈后阴道乳杆菌定植密度为(45.28±15.05)个/HP,乳杆菌分级正常比例为54.55%,H;O;阳性率81.82%,24 h pH值为4.49,24 h菌液浊度为46.6 MCF。VVC患者阴道乳杆菌定植密度为(48.53±10.59)个/HP,乳杆菌分级正常比例为73.33%,H;O;阳性率为80.00%,24 h pH值为4.41,24 h菌液浊度为52.2 MCF。VVC患者治愈后阴道乳杆菌定植密度为(47.95±10.28)个/HP,乳杆菌分级正常比例为90.48%,H;O;阳性率85.71%,24 h pH值为4.33,24 h菌液浊度为51.3 MCF。结论BV患者阴道乳杆菌功能减退,治愈后未能完全重建,需帮助恢复阴道乳杆菌功能状态。VVC患者阴道乳杆菌功能基本正常。
基金Supported by The National Natural Science Foundation of China,No.30471687Chinese Ministry of Science and Technology,No.2008CB517403
文摘AIM:To investigate the mechanisms of Lactobacillus plantarum(L.plantarum)action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into groups of sham-operation,bile duct ligation(BDL),BDL +L.plantarum,BDL+internal biliary drainage(IBD),and BDL+IBD+L.plantarum.Ten days after L.plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C(PKC)activity measurement.The distribution and expression of the PKC and tight junction(TJ)proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS:L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase(BDL vs BDL+L.plantarum,0.295±0.007 vs 0.349±0.003,P<0.05;BDL+IBD vs BDL+IBD+L.plantarum,0.407±0.046 vs 0.465±0.135,P<0.05),and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice(BDL vs BDL+L.plantarum,0.266±0.118 vs 0.326±0.009,P<0.05).The protective effect of L.plantarum was more prominent after internal biliary drainage(BDL+IBD vs BDL +IBD+L.plantarum,0.415±0.105 vs 0.494±0.145,P<0.05).CONCLUSION:L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.
