Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal brea...Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal breast tissue （NBT） using polymerase chain reaction （PCR）. Results The positive rates of HPV16 E6 , E7genes and CTSE were 60% （ 24/40 ） , 55% （ 22/40 ） and 67. 5% （ 27/40 ） respectively in BCs, whereas only 5% （1/20）, 5% （1/20） and 15% （3/20） in NBTs （ P 〈 O. 05）. There exited significant correlation between E6 gene and CTSE in BCs （ P 〈0. 05） , as well as E7 gene and CTSE. The infection of HPV16 E6, E7 and CTSE had no statistic relationship with pathological features. Conclusion There were HPV16 E6 , E7 genes and CTSE together in BCs and CTSE may play an important role in pathogenesis of BC.展开更多
Objective: The aim of the study was to discuss the diagnostic value of dynamic contrast enhanced-magnetic resonance imaging (DCE-MRI) in distinguishing malignant tumors of breast from benign lesions. Methods: A DCE-MR...Objective: The aim of the study was to discuss the diagnostic value of dynamic contrast enhanced-magnetic resonance imaging (DCE-MRI) in distinguishing malignant tumors of breast from benign lesions. Methods: A DCE-MRI study was performed on 18 benign lesions, 16 malignant tumors and 2 borderline lesions of breast (mean age 51.7 years). The seven items including shape, marginal feature, spreading signs, time-signal intensity curve, maximum slope of increase (MSI), signal enhancement ratio (SER) and signal enhanced extent (SEE) were compared between patients with benign lesions and those with malignant tumors. Results: Shape, marginal feature, spreading signs, time-signal intensity curve, SEE and SER between the benign and malignant groups of breast represented significantly difference except MSI. The 36 cases with breast lesion displayed sensitivity, specificity, accuracy for diagnosing malignant tumor of breast using the scoring method was 93.8%, 83.3% and 88.2%, respectively. Conclusion: DCE-MRI combined with morphology and dynamic enhanced feature of breast lesion has well efficacy to differentiate malignant tumor from benign lesions.展开更多
基金National Nature and Science Foundation, China (30500600)
文摘Objective To detect HPV16 E6, E7 genes and cell-type specific enhancer （CTSE） of long control region （LCR） in breast carcinoma （BC）. Methods HPV16 E6 ,E7 genes and CTSE were detected in 40 BCs and 20 normal breast tissue （NBT） using polymerase chain reaction （PCR）. Results The positive rates of HPV16 E6 , E7genes and CTSE were 60% （ 24/40 ） , 55% （ 22/40 ） and 67. 5% （ 27/40 ） respectively in BCs, whereas only 5% （1/20）, 5% （1/20） and 15% （3/20） in NBTs （ P 〈 O. 05）. There exited significant correlation between E6 gene and CTSE in BCs （ P 〈0. 05） , as well as E7 gene and CTSE. The infection of HPV16 E6, E7 and CTSE had no statistic relationship with pathological features. Conclusion There were HPV16 E6 , E7 genes and CTSE together in BCs and CTSE may play an important role in pathogenesis of BC.
基金Supported by a grant from the Sciences Foundation of West district, Beijing, China (No. 06027)
文摘Objective: The aim of the study was to discuss the diagnostic value of dynamic contrast enhanced-magnetic resonance imaging (DCE-MRI) in distinguishing malignant tumors of breast from benign lesions. Methods: A DCE-MRI study was performed on 18 benign lesions, 16 malignant tumors and 2 borderline lesions of breast (mean age 51.7 years). The seven items including shape, marginal feature, spreading signs, time-signal intensity curve, maximum slope of increase (MSI), signal enhancement ratio (SER) and signal enhanced extent (SEE) were compared between patients with benign lesions and those with malignant tumors. Results: Shape, marginal feature, spreading signs, time-signal intensity curve, SEE and SER between the benign and malignant groups of breast represented significantly difference except MSI. The 36 cases with breast lesion displayed sensitivity, specificity, accuracy for diagnosing malignant tumor of breast using the scoring method was 93.8%, 83.3% and 88.2%, respectively. Conclusion: DCE-MRI combined with morphology and dynamic enhanced feature of breast lesion has well efficacy to differentiate malignant tumor from benign lesions.
