目的观察雌激素(E2)对乳腺癌MCF7细胞中E-钙粘蛋白基因(CDH)的调控。方法使用Western blot、Real Time PCR分别在蛋白和RNA水平上观察雌激素如何影响E-钙粘蛋白。使用双荧光素酶报告系统检测雌激素如何影响E-钙粘蛋白启动子活性。结果...目的观察雌激素(E2)对乳腺癌MCF7细胞中E-钙粘蛋白基因(CDH)的调控。方法使用Western blot、Real Time PCR分别在蛋白和RNA水平上观察雌激素如何影响E-钙粘蛋白。使用双荧光素酶报告系统检测雌激素如何影响E-钙粘蛋白启动子活性。结果雌激素下调乳腺癌MCF7细胞中E-钙粘蛋白的蛋白和RNA水平,并且能够下调E-钙粘蛋白的启动子活性。结论雌激素下调乳腺癌细胞中E-钙粘蛋白基因,为进一步探讨乳腺癌发病机制提供有力证据。展开更多
Bee venom （BV） was used from long time ago in the medical field as treatment of chronic joint affections. In the recent decades, the screening process of new sources of antimicrobials discovers its high advantageous...Bee venom （BV） was used from long time ago in the medical field as treatment of chronic joint affections. In the recent decades, the screening process of new sources of antimicrobials discovers its high advantageous characteristics for combating various types of microbes, as well as trials to discover its anti-cancer medicinal fields. Lumpy skin disease virus （LSDV） causes disease in cattle of economic importance, and this work aimed to find treatment as well as alternative inactivant for LSDV. The use of bee venom as antiviral was experimented in this work and exhibited satisfied inhibitory effects on LSDV, meanwhile, the antigenic properties was still intact. The viability of virus was tested in tissue culture cells lines and in embryonated chicken eggs. According to doses and time of exposure, the cell lines of Hep-2 （human larynx carcinoma） and MCF7 （breast carcinoma cell line） were treated with different concentrations of BV and examined after 24 h post-inoculation. The Hep-2 and MCF7 cell lines were treated with various concentrations of BV in descending doses as follow： 25, 20, 15, 10, 5 and 0.5 ug/mL of BV. Then bee venom pathological effects on Hep-2 cells and MCF7 cells were observed, such as apoptosis, retarded growths and cytolysis. The results indicate the possibilities of using bee venom as anti-neoplastic and antiviral.展开更多
文摘目的观察雌激素(E2)对乳腺癌MCF7细胞中E-钙粘蛋白基因(CDH)的调控。方法使用Western blot、Real Time PCR分别在蛋白和RNA水平上观察雌激素如何影响E-钙粘蛋白。使用双荧光素酶报告系统检测雌激素如何影响E-钙粘蛋白启动子活性。结果雌激素下调乳腺癌MCF7细胞中E-钙粘蛋白的蛋白和RNA水平,并且能够下调E-钙粘蛋白的启动子活性。结论雌激素下调乳腺癌细胞中E-钙粘蛋白基因,为进一步探讨乳腺癌发病机制提供有力证据。
文摘Bee venom （BV） was used from long time ago in the medical field as treatment of chronic joint affections. In the recent decades, the screening process of new sources of antimicrobials discovers its high advantageous characteristics for combating various types of microbes, as well as trials to discover its anti-cancer medicinal fields. Lumpy skin disease virus （LSDV） causes disease in cattle of economic importance, and this work aimed to find treatment as well as alternative inactivant for LSDV. The use of bee venom as antiviral was experimented in this work and exhibited satisfied inhibitory effects on LSDV, meanwhile, the antigenic properties was still intact. The viability of virus was tested in tissue culture cells lines and in embryonated chicken eggs. According to doses and time of exposure, the cell lines of Hep-2 （human larynx carcinoma） and MCF7 （breast carcinoma cell line） were treated with different concentrations of BV and examined after 24 h post-inoculation. The Hep-2 and MCF7 cell lines were treated with various concentrations of BV in descending doses as follow： 25, 20, 15, 10, 5 and 0.5 ug/mL of BV. Then bee venom pathological effects on Hep-2 cells and MCF7 cells were observed, such as apoptosis, retarded growths and cytolysis. The results indicate the possibilities of using bee venom as anti-neoplastic and antiviral.
