“WHO肿瘤分类”蓝皮书是病理医师广为熟知、被奉为组织病理诊断“金标准”的经典参考书,该系列丛书已更新至第五版。近期,与之对应的细胞学蓝皮书——“WHO细胞病理学报告系统(WHO cytopathology reporting systems,WHO-CRS)”也已开...“WHO肿瘤分类”蓝皮书是病理医师广为熟知、被奉为组织病理诊断“金标准”的经典参考书,该系列丛书已更新至第五版。近期,与之对应的细胞学蓝皮书——“WHO细胞病理学报告系统(WHO cytopathology reporting systems,WHO-CRS)”也已开始推出,由国际细胞学会(International Academy of Cytology,IAC)、国际癌症研究机构(International Agency for Research on Cancer,IARC)以及WHO组成的联合编辑委员会(IAC-IARC-WHO Joint Editorial Board)负责编撰出版。展开更多
[Objective] The aim of this study was to optimize conditions of exogenous gene mediated by liposome to transfect yak mammary epithelial cells in Vitro.[Method] Yak mammary epithelial cells were isolated and cultivated...[Objective] The aim of this study was to optimize conditions of exogenous gene mediated by liposome to transfect yak mammary epithelial cells in Vitro.[Method] Yak mammary epithelial cells were isolated and cultivated in Vitro by the methods of collagenase digestion and tissue adhesion.The expression of cytokeratin in yak mammary epithelial cell was detected by immunocytochemistry technique.With green fluorescence protein as the report gene,yak mammary epithelial cells were transfected with exogenous gene m...展开更多
Objective: To evaluate the accuracy of these two methods and focus on the analysis and management of the false-negative cases. Methods: Results of full field digital mammography (FFDM) and fine needle aspiration cytol...Objective: To evaluate the accuracy of these two methods and focus on the analysis and management of the false-negative cases. Methods: Results of full field digital mammography (FFDM) and fine needle aspiration cytology (FNAC) were obtained and analyzed from a consecutive of 102 women with palpable breast masses, results were correlated with the histopathological findings. Results: Of the 102 cases, malignancy was confirmed in 43 cases (42.16%) by final pathological examination, the sensitivity and specificity of cancer detection with FNA cytology was 90.7% (39/43) and 89.8% (53/59), re- spectively, the whole accuracy was 90.2% (92/102), with a positive predictive value of 86.7% (39/45) and a negative predictive value of 93.0% (53/57). FFDM gave a sensitivity of 88.4% (38/43), specificity of 83.1% (49/59), and whole accuracy 85.3% (87/102), the positive predictive value and negative predictive value was 79.2% (38/48) and 90.7% (49/54), respectively. All the FNAC-negative cancer cases were suggestive of malignancy by FFDM findings, however, the benign cases which present as equivocal finding by FNA cytology, could not be ruled out the presence of malignancy. Conclusion: FNAC and FFDM both are accurate, effective and economical diagnostic modalities, combined use of these two methods can reduced the misdiag- nosis rate of breast masses.展开更多
Objective: To analyze the clinicopathologic features of breast signet-ring cell carcinoma (SRCC) presenting with genital tract metastasis. Methods: A 54-year-old woman presented with metrorrhagia was described and the...Objective: To analyze the clinicopathologic features of breast signet-ring cell carcinoma (SRCC) presenting with genital tract metastasis. Methods: A 54-year-old woman presented with metrorrhagia was described and the immunostaining was performed. Results: Histologically, signet ring cells (SRCs) scattered in the stroma of endometrium and cervix, bilateral fallopian tubes, ovaries and left breast. Immunohistochemistry of these SRCs showed strong reactive for cytokertin 7, CEA, GCDFP-15, and weak reactive for CA125 and negative for cytokertin 20. All these information confirmed the breast origin of SRCC. Conclusion: Primary breast SRCC is extremely rare with metrorrhagia as its first symptom. Gynecologist and pathologist should be aware of this possibility when metrorrhagia is the only symptom. Immunohistochemistry can help differentiate the origin of SRCC.展开更多
Primary clear cell carcinoma of the breast is a rare tumor with different morphological characteristics compared to ordinary breast carcinomas. The clear cell morphology of the neoplastic population in these tumors ha...Primary clear cell carcinoma of the breast is a rare tumor with different morphological characteristics compared to ordinary breast carcinomas. The clear cell morphology of the neoplastic population in these tumors has been ascribed to the presence of intracellular lipid, mucin or glycogen, or to myoepithelaial, apocrine, or neuroendocrine differentiation. We describe 2 cases of glycogen-rich clear cell carcinoma of the breast as follows.展开更多
文摘“WHO肿瘤分类”蓝皮书是病理医师广为熟知、被奉为组织病理诊断“金标准”的经典参考书,该系列丛书已更新至第五版。近期,与之对应的细胞学蓝皮书——“WHO细胞病理学报告系统(WHO cytopathology reporting systems,WHO-CRS)”也已开始推出,由国际细胞学会(International Academy of Cytology,IAC)、国际癌症研究机构(International Agency for Research on Cancer,IARC)以及WHO组成的联合编辑委员会(IAC-IARC-WHO Joint Editorial Board)负责编撰出版。
基金Supported by National Natural Science Foundation(30771550)~~
文摘[Objective] The aim of this study was to optimize conditions of exogenous gene mediated by liposome to transfect yak mammary epithelial cells in Vitro.[Method] Yak mammary epithelial cells were isolated and cultivated in Vitro by the methods of collagenase digestion and tissue adhesion.The expression of cytokeratin in yak mammary epithelial cell was detected by immunocytochemistry technique.With green fluorescence protein as the report gene,yak mammary epithelial cells were transfected with exogenous gene m...
文摘Objective: To evaluate the accuracy of these two methods and focus on the analysis and management of the false-negative cases. Methods: Results of full field digital mammography (FFDM) and fine needle aspiration cytology (FNAC) were obtained and analyzed from a consecutive of 102 women with palpable breast masses, results were correlated with the histopathological findings. Results: Of the 102 cases, malignancy was confirmed in 43 cases (42.16%) by final pathological examination, the sensitivity and specificity of cancer detection with FNA cytology was 90.7% (39/43) and 89.8% (53/59), re- spectively, the whole accuracy was 90.2% (92/102), with a positive predictive value of 86.7% (39/45) and a negative predictive value of 93.0% (53/57). FFDM gave a sensitivity of 88.4% (38/43), specificity of 83.1% (49/59), and whole accuracy 85.3% (87/102), the positive predictive value and negative predictive value was 79.2% (38/48) and 90.7% (49/54), respectively. All the FNAC-negative cancer cases were suggestive of malignancy by FFDM findings, however, the benign cases which present as equivocal finding by FNA cytology, could not be ruled out the presence of malignancy. Conclusion: FNAC and FFDM both are accurate, effective and economical diagnostic modalities, combined use of these two methods can reduced the misdiag- nosis rate of breast masses.
文摘Objective: To analyze the clinicopathologic features of breast signet-ring cell carcinoma (SRCC) presenting with genital tract metastasis. Methods: A 54-year-old woman presented with metrorrhagia was described and the immunostaining was performed. Results: Histologically, signet ring cells (SRCs) scattered in the stroma of endometrium and cervix, bilateral fallopian tubes, ovaries and left breast. Immunohistochemistry of these SRCs showed strong reactive for cytokertin 7, CEA, GCDFP-15, and weak reactive for CA125 and negative for cytokertin 20. All these information confirmed the breast origin of SRCC. Conclusion: Primary breast SRCC is extremely rare with metrorrhagia as its first symptom. Gynecologist and pathologist should be aware of this possibility when metrorrhagia is the only symptom. Immunohistochemistry can help differentiate the origin of SRCC.
文摘Primary clear cell carcinoma of the breast is a rare tumor with different morphological characteristics compared to ordinary breast carcinomas. The clear cell morphology of the neoplastic population in these tumors has been ascribed to the presence of intracellular lipid, mucin or glycogen, or to myoepithelaial, apocrine, or neuroendocrine differentiation. We describe 2 cases of glycogen-rich clear cell carcinoma of the breast as follows.
