目的探讨母乳能否与乙型肝炎表面抗原（hepatitis B surface antigen，HBsAg）结合以及结合特性。方法采集5例HBsAg和乙型肝炎表面抗体（hepatitis B surface antibody，抗-HBs）均阴性产妇产后1～2个月的母乳。分别用母乳全乳、乳脂和...目的探讨母乳能否与乙型肝炎表面抗原（hepatitis B surface antigen，HBsAg）结合以及结合特性。方法采集5例HBsAg和乙型肝炎表面抗体（hepatitis B surface antibody，抗-HBs）均阴性产妇产后1～2个月的母乳。分别用母乳全乳、乳脂和乳蛋白与酵母表达的高纯度HBsAg孵育，以牛奶和羊奶作为对照，酶联免疫吸附（enzyme—linked immunosorbent assay，ELISA）法检测各成分与HBsAg的结合能力；化学发光微粒子免疫分析法检测各种乳汁对HBsAg与抗HBs结合的抑制率；温度敏感试验[包括煮沸1min、巴氏消毒（65℃、30min）及不加热处理1检测母乳中与HBsAg结合成分的热稳定性。采用单因素方差分析和SNK法进行统计学分析。结果本研究选择0．11ag／ml的HBsAg为适当工作浓度进行试验。5例母乳均能明显与HBsAg结合（A450，1．306±0．300），而牛奶及羊奶与HBsAg的结合能力不明显（分别为2．157±0．150和2．232±0．093），差异有统计学意义（F=34．303，P〈0．01）。定量试验显示，母乳抑制HBsAg与抗HBs结合的抑制率为（74．26±17．26）％，高于PBS的（0．00±5．50）％，差异有统计学意义（F=57．806，P〈0．01）。乳脂、脱脂乳及全乳均能与HBsAg结合（A450分别为0．877±0．486、0．513±0．069和0．376±0．146，F=44．475，P〈0．01）。经煮沸1min或巴氏消毒处理后，母乳全乳与HBsAg的结合能力均无明显变化；而脱脂乳结合能力有所降低（F=16．598，P〈0．01）；母乳全乳和脱脂乳均能明显抑制HBsAg与抗-HBs的结合（F值分别为278．341和269．408，P值均〈0．01）。结论母乳具有与HBsAg结合的特性，其活性成分主要存在于乳蛋白，并具有热稳定性。母乳蛋白具有与HBV结合的能力。展开更多
Objective To investigate the marker protein of human breast carcinoma from nuclear matrix proteins (NMPs). Methods NMPs were injected subcutaneously into rabbit to get antiserum, which was used to detect the NMPs spec...Objective To investigate the marker protein of human breast carcinoma from nuclear matrix proteins (NMPs). Methods NMPs were injected subcutaneously into rabbit to get antiserum, which was used to detect the NMPs specificity for breast carcinoma. Results There was an apparent positive band (100 kD) in the NMPs of breast carcinoma, which did not exist in normal breast and other tumors that were detected.Conclusion One or one group of 100 kD NMPs were found to be related to human breast carcinoma, which may be involved in the carcinogenesis and development of human breast carcinoma and valuable for breast carcinoma diagnosis.展开更多
文摘目的探讨母乳能否与乙型肝炎表面抗原（hepatitis B surface antigen，HBsAg）结合以及结合特性。方法采集5例HBsAg和乙型肝炎表面抗体（hepatitis B surface antibody，抗-HBs）均阴性产妇产后1～2个月的母乳。分别用母乳全乳、乳脂和乳蛋白与酵母表达的高纯度HBsAg孵育，以牛奶和羊奶作为对照，酶联免疫吸附（enzyme—linked immunosorbent assay，ELISA）法检测各成分与HBsAg的结合能力；化学发光微粒子免疫分析法检测各种乳汁对HBsAg与抗HBs结合的抑制率；温度敏感试验[包括煮沸1min、巴氏消毒（65℃、30min）及不加热处理1检测母乳中与HBsAg结合成分的热稳定性。采用单因素方差分析和SNK法进行统计学分析。结果本研究选择0．11ag／ml的HBsAg为适当工作浓度进行试验。5例母乳均能明显与HBsAg结合（A450，1．306±0．300），而牛奶及羊奶与HBsAg的结合能力不明显（分别为2．157±0．150和2．232±0．093），差异有统计学意义（F=34．303，P〈0．01）。定量试验显示，母乳抑制HBsAg与抗HBs结合的抑制率为（74．26±17．26）％，高于PBS的（0．00±5．50）％，差异有统计学意义（F=57．806，P〈0．01）。乳脂、脱脂乳及全乳均能与HBsAg结合（A450分别为0．877±0．486、0．513±0．069和0．376±0．146，F=44．475，P〈0．01）。经煮沸1min或巴氏消毒处理后，母乳全乳与HBsAg的结合能力均无明显变化；而脱脂乳结合能力有所降低（F=16．598，P〈0．01）；母乳全乳和脱脂乳均能明显抑制HBsAg与抗-HBs的结合（F值分别为278．341和269．408，P值均〈0．01）。结论母乳具有与HBsAg结合的特性，其活性成分主要存在于乳蛋白，并具有热稳定性。母乳蛋白具有与HBV结合的能力。
基金Supported by the National Natural Science Foundation of China (No.30500600)
文摘Objective To investigate the marker protein of human breast carcinoma from nuclear matrix proteins (NMPs). Methods NMPs were injected subcutaneously into rabbit to get antiserum, which was used to detect the NMPs specificity for breast carcinoma. Results There was an apparent positive band (100 kD) in the NMPs of breast carcinoma, which did not exist in normal breast and other tumors that were detected.Conclusion One or one group of 100 kD NMPs were found to be related to human breast carcinoma, which may be involved in the carcinogenesis and development of human breast carcinoma and valuable for breast carcinoma diagnosis.
