全血L-乳酸生物传感器

全血L 乳酸生物传感器适用于快速方便地测量全血中L 乳酸的浓度。它包括印有一对碳电极的基片 ,由L 乳酸氧化酶和作为电子传递剂的铁氰化钾构成的反应层和塑料薄膜形成的反应腔。将血样 (2 μL)滴在反应腔中 ,然后在电极两端施加一个 30 0mV的恒电压 ,5 0s后读取电流值 ,即可得到对应的L 乳酸浓度。该传感器的线性范围为 0～ 15mol。保存 12 0d后 ,响应没有明显衰减。该传感器的测量结果和美国强生VITROS2 5 0SYSTEM全自动干化学分析仪的测量结果有良好的相关性 (r=0 980 9) 。

Prognostic values of serum LDH and β2-MG in patients with non-Hodgkin’s lymphoma

Objective： To evaluate the clinical significance of quantitative determination of serum lactic dehydrogenase （LDH） and beta 2-microgtobulin （β2-MG） in patients with non-Hodgkin＇s lymphoma （NHL）. Methods： Date of the 65 patients from 2003 to 2004 were retrospectively analyzed, relationship between LDH and β2-MG activities and tumor phase, effect of treat- ment as well as prognosis were studied. Results： The levels of LDH and β2-MG in the advanced stage, B group and bone marrow involvement were obviously higher than their comparison counterpart and the difference was significant （P 〈 0.05）. There was no significant difference between the high LDH group and normal LDH group in the two-year survival rate. But the difference in the four-year survival rate was significance. Conclusion： The level of LDH and β2-MG are valuable for clinical stage, prognosis and evaluation of therapeutic response in patients with NHL.