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胞壁酰肽及其类似物的FABMS研究
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作者 傅桂香 俞璐 +1 位作者 张明珠 徐杰诚 《质谱学报》 EI CAS CSCD 1995年第3期22-26,共5页
本文报道12个合成的胞壁酰肽及其类似物的FABMS.所有化合物都出现质子化分子离子[M+H] ̄+和[M+Na] ̄+峰,碎片离子的形成主要是由MH ̄+脱水或脱保扩基,可以清晰地观察到序列离子,另外由分子离子失去完整的吡... 本文报道12个合成的胞壁酰肽及其类似物的FABMS.所有化合物都出现质子化分子离子[M+H] ̄+和[M+Na] ̄+峰,碎片离子的形成主要是由MH ̄+脱水或脱保扩基,可以清晰地观察到序列离子,另外由分子离子失去完整的吡喃糖可生成乳酸肽链离子。 展开更多
关键词 胞壁酰肽 乳酸肽链 质谱 FABMS
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A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example 被引量:3
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作者 WANG Fang CAO Li-ting HU Song-hua 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第8期549-554,共6页
The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins... The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances. 展开更多
关键词 MTT colorimetric assay NISIN Micrococcus luteus
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