A study on the activity of lactate dehydrogenase isoenzyme C4(LDH-C4) in spermatozoa and seminal plasma was performed in the following subjects: normal males were recruited as control, infertile men with or without no...A study on the activity of lactate dehydrogenase isoenzyme C4(LDH-C4) in spermatozoa and seminal plasma was performed in the following subjects: normal males were recruited as control, infertile men with or without normal semen quality were recruited as groups Inf. I and Inf. H, oligospermic patients were recruited as the 4th group. The ratios of LDH-C4 in seminal plasma to that in spermatozoa were 0.528(Pso) in the control, 0.88, 2.85 and 4.01 in Inf, I, H and oligospermic groups respectivel.v. All differences between the normal and the other groups were significant(P <0.005). The activity of LDH-C4 in spermatozoa might become an important biochemical index for estimation of semen quality and male fertility. A good correlation between LDH-C4 in spermatozoa was found (r = 0.9593).展开更多
The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation unde...The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation under well-defined experimental conditions. The experiments were conducted in a specialized microwave processing apparatus, with an exposure frequency of 18 GHz, and a temperature profile that was restricted to below 40℃ to avoid thermal degradation of the bacteria. The absorbed power was calculated to be 1,500 kW/m3 and the electric field was determined to be 300 Wm. Both values were theoretically confirmed using Computer Simulation Technology （CST） Microwave Studio 3D Electromagnetic Stimulation Software. Results showed that the activity of both enzymes was increased following MW radiation compared to negative controls and thermally treated samples subjected to similar temperature profiles. It is suggested that the increase in COX and LDH enzyme activity could not be explained by conventional heating alone, but was rather a result of micro-thermal effects that incorporated ＇undetectable＇ thermal mechanisms.展开更多
文摘A study on the activity of lactate dehydrogenase isoenzyme C4(LDH-C4) in spermatozoa and seminal plasma was performed in the following subjects: normal males were recruited as control, infertile men with or without normal semen quality were recruited as groups Inf. I and Inf. H, oligospermic patients were recruited as the 4th group. The ratios of LDH-C4 in seminal plasma to that in spermatozoa were 0.528(Pso) in the control, 0.88, 2.85 and 4.01 in Inf, I, H and oligospermic groups respectivel.v. All differences between the normal and the other groups were significant(P <0.005). The activity of LDH-C4 in spermatozoa might become an important biochemical index for estimation of semen quality and male fertility. A good correlation between LDH-C4 in spermatozoa was found (r = 0.9593).
文摘The catalytic activity of two common bacterial enzymes, lactate dehydrogenase （LDH） and cytochrome c oxidase （COX） from Escherichia coli was examined following bacterial exposure to microwave （MW） radiation under well-defined experimental conditions. The experiments were conducted in a specialized microwave processing apparatus, with an exposure frequency of 18 GHz, and a temperature profile that was restricted to below 40℃ to avoid thermal degradation of the bacteria. The absorbed power was calculated to be 1,500 kW/m3 and the electric field was determined to be 300 Wm. Both values were theoretically confirmed using Computer Simulation Technology （CST） Microwave Studio 3D Electromagnetic Stimulation Software. Results showed that the activity of both enzymes was increased following MW radiation compared to negative controls and thermally treated samples subjected to similar temperature profiles. It is suggested that the increase in COX and LDH enzyme activity could not be explained by conventional heating alone, but was rather a result of micro-thermal effects that incorporated ＇undetectable＇ thermal mechanisms.
