The genetically modified high-oleic rapeseed （Brassica napus L.） line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar We...The genetically modified high-oleic rapeseed （Brassica napus L.） line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar Westar.The transformation was mediated by Agrobacterium.The flanking sequences to both the left and right borders of T-DNA insertion site were amplified by thermal asymmetric interlaced PCR （TAIL-PCR） from the genomic DNA of the transgenic rapeseed line W-4.The flanking sequences to the right border was 290 bp in length and the nucleotide composition was 31.27％ for G＋C content while 68.73％ for A＋T content.The flanking sequence to the left border was 365 bp in length and the G＋C content was 32.6％ and the A＋T content was 67.4％,indicating that the T-DNA was integrated in the A/T-rich region.Further more,sequence alignment analysis showed a deletion of 62 bp including the right border of pCNFIRnos and the integration of the whole left border except a change of G to A.That was to say,the integration of the T-DNA in the transgenic line W-4 not involved in the vector sequences.Based on both flanking sequences as well as the left and right borders of the T-DNA sequences,two pairs of specific primers TLF/TLR and TRF/TRR were designed.Using the primers the event-specific PCR detection method for transgenic rapeseed line W-4 was established.By the PCR,two fragments of 485 and 405 bp were amplified from the W-4 genomic DNA as expected,while no products were amplified from the genomic DNA of other transgenic rapeseed lines and non-transgenic rapeseed line.And by the PCR it is possible to detect the W-4 genomic DNA from a mixed sample of genomic DNA.The limit of the detection for the qualitative PCR assay was 0.1％.The method developed in this work is highly specific,sensitive and suitable for event-specific detection of the transgenic rapeseed line W-4.展开更多
<Abstract>This paper reports the seasonal feature of the relationship between ENSO and the stratospheric Polar Vortex Oscillation (PVO) variability in the Northern Hemisphere.It is shown that the lagged ENSO-PVO...<Abstract>This paper reports the seasonal feature of the relationship between ENSO and the stratospheric Polar Vortex Oscillation (PVO) variability in the Northern Hemisphere.It is shown that the lagged ENSO-PVO coupling relationship exhibits distinct seasonal feature,due to the strong seasonality of PVO and ENSO.Specifically,the PVO variability not only during winter,but also in autumn and spring months,is significantly correlated with ENSO anomalies leading by seasons;however,no significant effect of ENSO is found on the PVO variability in winter months of November and February.Although a significant ENSO effect is primarily observed when ENSO leads PVO by about one year,a significant correlation is also found between PVO in the following spring months (M +1 A +1) and ENSO anomalies in the previous autumn (A-1 S-1 O- 1 N -1) when ENSO anomalies lead by about 18 months.The significant correlation between PVO in various seasons and the corresponding ENSO anomalies leading by seasons could be explicitly verified in most of the individual years,confirming that the lagged ENSO effect can largely modulate the seasonal timescale variability of PVO.Moreover,the composite spatial patterns of the zonal-mean temperature anomalies further show that the ENSO effect on the PVO in various seasons is related to the interannual variability of the seasonal timescale PVO events.展开更多
Northeast China experiences severe atmospheric pollution, with an increasing occurrence of heavy haze episodes. However, the underlying forces driving haze formation during different seasons are poorly understood. In ...Northeast China experiences severe atmospheric pollution, with an increasing occurrence of heavy haze episodes. However, the underlying forces driving haze formation during different seasons are poorly understood. In this study, we explored the spatio-temporal characteristics and causes of haze events in Northeast China by combining a range of data sources(i.e., ground monitoring, satellite-based products, and meteorological products). It was found that the ‘Shenyang-Changchun-Harbin(SCH)'city belt was the most polluted area in the region on an annual scale. The spatial distribution of air quality index(AQI) values had a clear seasonality, with the worst pollution occurring in winter, an approximately oval-shaped polluted area around western Jilin Province in spring, and the best air quality occurring in summer and most of the autumn. The three periods that typically experienced intense haze events were Period I from mid-October to mid-November(i.e., late autumn and early winter), Period II from late-December to February(i.e., the coldest time in winter), and Period III from April to mid-May(i.e., spring). During Period I, strong PM_(2.5) emissions from seasonal crop residue burning and coal burning for winter heating were the dominant reasons for the occurrence of extreme haze events(AQI > 300). Period II had frequent heavy haze events(200 < AQI < 300) in the coldest months of January and February, which were due to high PM_(2.5) emissions from coal burning and vehicle fuel consumption, a lower atmospheric boundary layer, and stagnant atmospheric conditions. Haze events in Period III, with high PM_(10) concentrations, were primarily caused by the regional transportation of windblown dust from degraded grassland in central Inner Mongolia and bare soil in western Jilin Province. Local agricultural tilling could also release PM_(10) and enhance the levels of windblown dust from tilled soil. Better control of coal burning, fuel consumption, and crop residue burning in winter and autumn is urgently needed to address the haze problem in Northeast China.展开更多
The literature on disasters mainly focuses on natural disasters and looks at their coverage by the media. The paper argues that armed conflicts are also disasters and that Western relief agencies intervening in natura...The literature on disasters mainly focuses on natural disasters and looks at their coverage by the media. The paper argues that armed conflicts are also disasters and that Western relief agencies intervening in natural disasters and conflict are also proactive communicators. Taking a different approach from much of the available research, it explores agencies＇ communication work in these two types of crises and analyses the differences, challenges and dilemmas that they face in communicating their humanitarianism. It outlines the distinguished inherent characteristics of natural disasters and conflicts, and examines their impact on the understanding of the crises, the perception of affected populations, and the implementation of agencies＇ actions. It also investigates how, in turn, all these features influence agencies＇ communication practices that in natural disasters emergencies and conflict-related crises perform a different role and show different parameters. In this sense, the paper distinguishes between ＂humanitarian communication＂ to refer to the former, and ＂humanitarian advocacy＂ to refer to the latter. The paper briefly explores the operational challenges engendered by the contested interpretations of humanitarian advocacy, shows that the use of the parameters of humanitarian communication in conflict-related crises to overcome these problems caused additional concern, and presents two forms of humanitarian advocacy engendering less challenges and dilemmas.展开更多
In this paper, the characterization of single event multiple cell upsets(MCUs) in a custom SRAM is performed in a 65 nm triple-well CMOS technology, and O(linear energy transfer(LET) = 3.1 Me V cm2/mg), Ti(LET = 22.2 ...In this paper, the characterization of single event multiple cell upsets(MCUs) in a custom SRAM is performed in a 65 nm triple-well CMOS technology, and O(linear energy transfer(LET) = 3.1 Me V cm2/mg), Ti(LET = 22.2 Me V cm2/mg) and Ge(LET = 37.4 Me V cm2/mg) particles are employed. The experimental results show that the percentage of MCU events in total upset events is 71.11%, 83.47% and 85.53% at O, Ti and Ge exposures. Moreover, due to the vertical well isolation layout, 100%(O), 100%(Ti) and 98.11%(Ge) MCU cluster just present at one or two adjacent columns, but there are still 4 cell upsets in one MCU cluster appearing on the same word wire. The characterization indicates that MCUs have become the main source of soft errors in SRAM, and even though combining the storage array interleaving distance(ID) scheme with the error detection and correction(EDAC) technique, the MCUs cannot be completely eliminated, new radiation hardened by design techniques still need to be further studied.展开更多
Nanopore has been developed to be a powerful,single-molecule analytical tool for sensing ions,small organic molecules and biomacromolecules such as proteins and DNAs.Generally,the identity of the analyte can be reveal...Nanopore has been developed to be a powerful,single-molecule analytical tool for sensing ions,small organic molecules and biomacromolecules such as proteins and DNAs.Generally,the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events.In some cases,generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level.However,we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through?-hemolysin nanopore.In this report,we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events.This finding might be useful in other secondary structure-related nanopore sensing activities.展开更多
基金Supported by Key Agricultural Technology Research and Development Program of Jiangsu Province(BE2009304)Fund for National Rapeseed Research System(CARS13)~~
文摘The genetically modified high-oleic rapeseed （Brassica napus L.） line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar Westar.The transformation was mediated by Agrobacterium.The flanking sequences to both the left and right borders of T-DNA insertion site were amplified by thermal asymmetric interlaced PCR （TAIL-PCR） from the genomic DNA of the transgenic rapeseed line W-4.The flanking sequences to the right border was 290 bp in length and the nucleotide composition was 31.27％ for G＋C content while 68.73％ for A＋T content.The flanking sequence to the left border was 365 bp in length and the G＋C content was 32.6％ and the A＋T content was 67.4％,indicating that the T-DNA was integrated in the A/T-rich region.Further more,sequence alignment analysis showed a deletion of 62 bp including the right border of pCNFIRnos and the integration of the whole left border except a change of G to A.That was to say,the integration of the T-DNA in the transgenic line W-4 not involved in the vector sequences.Based on both flanking sequences as well as the left and right borders of the T-DNA sequences,two pairs of specific primers TLF/TLR and TRF/TRR were designed.Using the primers the event-specific PCR detection method for transgenic rapeseed line W-4 was established.By the PCR,two fragments of 485 and 405 bp were amplified from the W-4 genomic DNA as expected,while no products were amplified from the genomic DNA of other transgenic rapeseed lines and non-transgenic rapeseed line.And by the PCR it is possible to detect the W-4 genomic DNA from a mixed sample of genomic DNA.The limit of the detection for the qualitative PCR assay was 0.1％.The method developed in this work is highly specific,sensitive and suitable for event-specific detection of the transgenic rapeseed line W-4.
基金supported by the National Basic Research Program of China under Grants 2010CB428603and2010CB950400100 Talents Program of the Chinese Academy of Sciences under Grant KZCX2-YW-BR-14
文摘<Abstract>This paper reports the seasonal feature of the relationship between ENSO and the stratospheric Polar Vortex Oscillation (PVO) variability in the Northern Hemisphere.It is shown that the lagged ENSO-PVO coupling relationship exhibits distinct seasonal feature,due to the strong seasonality of PVO and ENSO.Specifically,the PVO variability not only during winter,but also in autumn and spring months,is significantly correlated with ENSO anomalies leading by seasons;however,no significant effect of ENSO is found on the PVO variability in winter months of November and February.Although a significant ENSO effect is primarily observed when ENSO leads PVO by about one year,a significant correlation is also found between PVO in the following spring months (M +1 A +1) and ENSO anomalies in the previous autumn (A-1 S-1 O- 1 N -1) when ENSO anomalies lead by about 18 months.The significant correlation between PVO in various seasons and the corresponding ENSO anomalies leading by seasons could be explicitly verified in most of the individual years,confirming that the lagged ENSO effect can largely modulate the seasonal timescale variability of PVO.Moreover,the composite spatial patterns of the zonal-mean temperature anomalies further show that the ENSO effect on the PVO in various seasons is related to the interannual variability of the seasonal timescale PVO events.
基金Under the auspices of National Key R&D Program of China(No.2017YFC0212303,2017YFC0212304,2017YFC0212301)Key Research Program of Frontier Sciences,Chinese Academy of Sciences(No.QYZDB-SSW-DQC045)+1 种基金Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2017275)National Natural Science Foundation of China(No.41775116,41771071,41575129)
文摘Northeast China experiences severe atmospheric pollution, with an increasing occurrence of heavy haze episodes. However, the underlying forces driving haze formation during different seasons are poorly understood. In this study, we explored the spatio-temporal characteristics and causes of haze events in Northeast China by combining a range of data sources(i.e., ground monitoring, satellite-based products, and meteorological products). It was found that the ‘Shenyang-Changchun-Harbin(SCH)'city belt was the most polluted area in the region on an annual scale. The spatial distribution of air quality index(AQI) values had a clear seasonality, with the worst pollution occurring in winter, an approximately oval-shaped polluted area around western Jilin Province in spring, and the best air quality occurring in summer and most of the autumn. The three periods that typically experienced intense haze events were Period I from mid-October to mid-November(i.e., late autumn and early winter), Period II from late-December to February(i.e., the coldest time in winter), and Period III from April to mid-May(i.e., spring). During Period I, strong PM_(2.5) emissions from seasonal crop residue burning and coal burning for winter heating were the dominant reasons for the occurrence of extreme haze events(AQI > 300). Period II had frequent heavy haze events(200 < AQI < 300) in the coldest months of January and February, which were due to high PM_(2.5) emissions from coal burning and vehicle fuel consumption, a lower atmospheric boundary layer, and stagnant atmospheric conditions. Haze events in Period III, with high PM_(10) concentrations, were primarily caused by the regional transportation of windblown dust from degraded grassland in central Inner Mongolia and bare soil in western Jilin Province. Local agricultural tilling could also release PM_(10) and enhance the levels of windblown dust from tilled soil. Better control of coal burning, fuel consumption, and crop residue burning in winter and autumn is urgently needed to address the haze problem in Northeast China.
文摘The literature on disasters mainly focuses on natural disasters and looks at their coverage by the media. The paper argues that armed conflicts are also disasters and that Western relief agencies intervening in natural disasters and conflict are also proactive communicators. Taking a different approach from much of the available research, it explores agencies＇ communication work in these two types of crises and analyses the differences, challenges and dilemmas that they face in communicating their humanitarianism. It outlines the distinguished inherent characteristics of natural disasters and conflicts, and examines their impact on the understanding of the crises, the perception of affected populations, and the implementation of agencies＇ actions. It also investigates how, in turn, all these features influence agencies＇ communication practices that in natural disasters emergencies and conflict-related crises perform a different role and show different parameters. In this sense, the paper distinguishes between ＂humanitarian communication＂ to refer to the former, and ＂humanitarian advocacy＂ to refer to the latter. The paper briefly explores the operational challenges engendered by the contested interpretations of humanitarian advocacy, shows that the use of the parameters of humanitarian communication in conflict-related crises to overcome these problems caused additional concern, and presents two forms of humanitarian advocacy engendering less challenges and dilemmas.
基金supported by the National Natural Science Foundation of China(Grant No.61504169)the Preliminary Research Program of National University of Defense Technology of China(Grant No.0100066314001)
文摘In this paper, the characterization of single event multiple cell upsets(MCUs) in a custom SRAM is performed in a 65 nm triple-well CMOS technology, and O(linear energy transfer(LET) = 3.1 Me V cm2/mg), Ti(LET = 22.2 Me V cm2/mg) and Ge(LET = 37.4 Me V cm2/mg) particles are employed. The experimental results show that the percentage of MCU events in total upset events is 71.11%, 83.47% and 85.53% at O, Ti and Ge exposures. Moreover, due to the vertical well isolation layout, 100%(O), 100%(Ti) and 98.11%(Ge) MCU cluster just present at one or two adjacent columns, but there are still 4 cell upsets in one MCU cluster appearing on the same word wire. The characterization indicates that MCUs have become the main source of soft errors in SRAM, and even though combining the storage array interleaving distance(ID) scheme with the error detection and correction(EDAC) technique, the MCUs cannot be completely eliminated, new radiation hardened by design techniques still need to be further studied.
基金the National Basic Research Program of China (2013CB932800)the National Natural Science Foundation of China (21175135, 21375130, 21205119, 21475132)the CAS Hundred Talents Program
文摘Nanopore has been developed to be a powerful,single-molecule analytical tool for sensing ions,small organic molecules and biomacromolecules such as proteins and DNAs.Generally,the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events.In some cases,generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level.However,we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through?-hemolysin nanopore.In this report,we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events.This finding might be useful in other secondary structure-related nanopore sensing activities.
