A rational equation of state of the perturbation type with a repulsion and attraction term has been applied to reproduce critical curves of six different binary systems up to high temperatures and pressures. A square ...A rational equation of state of the perturbation type with a repulsion and attraction term has been applied to reproduce critical curves of six different binary systems up to high temperatures and pressures. A square well potential for intermolecular interaction is used. With pairwise combination rules for these potentials three adjustable parameters are needed. The experimental critical point and phase equilibrium data are compared with the values predicted using the equation of state. Good agreement is obtained for the analysis of the critical pressure composition data and molar volumes.展开更多
Aboveground vertical profiles of N2O concentrations were measured with in two natural coniferous-deciduous mixed forests of 1998 and 1999 in Changbai M ountain. Significant high N2O concentrations were found in six pr...Aboveground vertical profiles of N2O concentrations were measured with in two natural coniferous-deciduous mixed forests of 1998 and 1999 in Changbai M ountain. Significant high N2O concentrations were found in six profiles out of t welve profiles. The results showed that high concentrations were 3.03% to 64.9% higher than the "normal concentrations" in these six profiles. Differences betwe en the high concentrations and the "normal concentrations" were statistically si gnificant. The simultaneous occurrence of high concentrations at/nearby the cano py height and normal concentrations at the trunk space height indicated an efflu x of N2O from foliage to atmosphere. This study afforded evidence supporting tha t plant per se, besides forest soil, was an important source of atmospheric N2O in a forest ecosystem.展开更多
Identifying the driving forces that cause changes in forest ecosystem services related to water conservation is essential for the design of interventions that could enhance positive impacts as well as minimizing negat...Identifying the driving forces that cause changes in forest ecosystem services related to water conservation is essential for the design of interventions that could enhance positive impacts as well as minimizing negative impacts. In this study, we propose an assessment concept framework model for indirect-direct-ecosystem service (IN-DI-ESS) driving forces within this context and method for index construction that considers the selection of a robust and parsimonious variable set. Factor analysis was integrated into two-stage data envelopment analysis (TS-DEA) to determine the driving forces and their effects on water conservation services in forest ecosystems at the provincial scale in China. The results showed the following. 1) Ten indicators with factor scores more than 0.8 were selected as the minimum data set. Four indicators comprising population density, per capita gross domestic product, irrigation efficiency, and per capita food consumption were the indirect driving factors, and six indicators comprising precipitation, farmland into forestry or pasture, forest cover, habitat area, water footprint, and wood extraction were the direct driving forces. 2) Spearman's rank correlation test was performed to compare the overall effectiveness in two periods: stage 1 and stage 2. The calculated coefficients were 0.245, 0.136, and 0.579, respectively, whereas the tabulated value was 0.562. This indicates that the driving forces obviously differed in terms of their contribution to the overall effectiveness and they caused changes in water conservation services in different stages. In terms of the variations in different driving force effects in the years 2000 and 2010, the overall, stage 1, and stage 2 variances were 0.020, 0.065, and 0.079 in 2000, respectively, and 0.018, 0.063, and 0.071 in 2010. This also indicates that heterogeneous driving force effects were obvious in the process during the same period. Identifying the driving forces that affect service changes and evaluating their efficiency have significant policy implications for the management of forest ecosystem services. Advanced effectiveness measures for weak regions could be improved in an appropriate manner. In this study, we showed that factor analysis coupled with TS-DEA based on the IN-D1-ESS framework can increase the parsimony of driving force indicators, as well as interpreting the interactions among indirect and direct driving forces with forest ecosystem water conservation services, and reducing the uncertainty related to the internal consistency during data selection.展开更多
Mostly based on assumptions derived from controlled-environment studies, predicted future atmospheric CO2 concentrations [CO2] are expected to have considerable impacts on carbon (C) turnover in agro-ecosystems. In ...Mostly based on assumptions derived from controlled-environment studies, predicted future atmospheric CO2 concentrations [CO2] are expected to have considerable impacts on carbon (C) turnover in agro-ecosystems. In order to allow the in situ examination of C-transformations in the plant-soil system of arable crop rotations under future [002], a free air carbon dioxide enrichment (FACE) experiment (550 μmol mol^-1 CO2) was started at Braunschweig, Germany in 1999. The crop rotation under investigation comprised winter barley, a cover crop (ryegrass), sugar beets and winter wheat. Assessments of CO2 effects included the determination of above- and belowground biomass production, measurements of canopy CO2- and H2O- fluxes, soil microbial biomass and in situ soil respiration. The results obtained during the 1st crop rotation cycle (3 years) showed that for the selected crops elevated [CO2] entailed significant positive effects (P 〈 0.05) on aboveground (6%-14% stimulation) and belowground biomass production (up to 90% stimulation), while canopy evapotranspiration was reduced. This resulted in increased soil water content. Also, depending on crop type and season, high CO2 stimulated in situ soil respiration (up to 30%), while soil microbial biomass did not show significant respouses to elevated [CO2] during the first rotation cycle.展开更多
It is critical to study how different forest management practices affect forest carbon sequestration under global climate change regime. Previous researches focused on the stand-level forest carbon sequestration with ...It is critical to study how different forest management practices affect forest carbon sequestration under global climate change regime. Previous researches focused on the stand-level forest carbon sequestration with rare investigation of forest carbon stocks influ- enced by forest management practices and climate change at regional scale. In this study, a general integrative approach was used to simulate spatial and temporal variations of woody biomass and harvested biomass of forest in China during the 21st century under dif- ferent scenarios of climate and CO2 concentration changes and management tasks by coupling Integrated Terrestrial Ecosystem Carbon budget (InTEC) model with Global Forest Model (G4M). The results showed that forest management practices have more predominant effects on forest stem stocking biomass than climate and CO2 concentration change. Meanwhile, the concurrent future changes in cli- mate and CO2 concentration will enhance the amounts of stem stocking biomass in forests of China by 12%-23% during 2001-2100 relative to that with climate change only. The task for maximizing stem stocking biomass will dramatically enhance the stem stocking biomass from 2001~100, while the task for maximum average increment will result in an increment of stem stocking biomass before 2050 then decline. The difference of woody biomass responding to forest management tasks was owing to the current age structure of forests in China. Meanwhile, the sensitivity of long-term woody biomass to management practices for different forest types (coniferous forest, mixed forest and deciduous forest) under changing climate and CO2 concentration was also analyzed. In addition, longer rotation length under future climate change and rising CO2 concentration scenario will dramatically increase the woody biomass of China during 2001~100. Therefore, our estimation indicated that taking the role of forest management in the carbon cycle into the consideration at regional or national level is very important to project the forest carbon sequestration under future climate change and rising atmospheric CO2 concentration.展开更多
Soil organic carbon(SOC)can act as a sink or source of atmospheric carbon dioxide;therefore,it is important to understand the amount and composition of SOC in terrestrial ecosystems,the spatial variation in SOC,and th...Soil organic carbon(SOC)can act as a sink or source of atmospheric carbon dioxide;therefore,it is important to understand the amount and composition of SOC in terrestrial ecosystems,the spatial variation in SOC,and the underlying mechanisms that stabilize SOC.In this study,density fractionation and acid hydrolysis were used to assess the spatial variation in SOC,the heavy fraction of organic carbon(HFOC),and the resistant organic carbon(ROC)in soils of the southern Hulun Buir region,northeastern China,and to identify the major factors that contribute to this variation.The results showed that as the contents of clay and silt particles(0–50μm)increased,both methylene blue(MB)adsorption by soil minerals and microaggregate contents increased in the 0–20 and 20–40 cm soil layers(P<0.05).Although varying with vegetation types,SOC,HFOC,and ROC contents increased significantly with the content of clay and silt particles, MB adsorption by soil minerals,and microaggregate content(P<0.05),suggesting that soil texture,the MB adsorption by soil minerals,and microaggregate abundance might be important factors influencing the spatial heterogeneity of carbon contents in soils of the southern Hulun Buir region.展开更多
The measurement of CO2 and N2O effiux from forest soils is of great importance in evaluating the role of forests as sequestering agents of atmospheric CO2 and nitrogen. To quantify the effect of site on temperature de...The measurement of CO2 and N2O effiux from forest soils is of great importance in evaluating the role of forests as sequestering agents of atmospheric CO2 and nitrogen. To quantify the effect of site on temperature dependence of net C-mineralization and N2O-N emissions, three adjacent forest floors under beech, Norway spruce and mixed species stands were investigated at Soiling forest, Germany, by an incubation experiment for three months. The investigated net C-mineralization and N2O-N emissions from all forest floors exhibited an exponential increase with respect to temperature elevation. The temperature coefficient function (Q10 value), was fitted to flux rates to describe the temperature sensitivity of forest floors on temperature in the range of 1-20℃. Comparing the fitted curves for temperature sensitivity of the forest floors in relation to net carbon mineralization and nitrous oxide emission rates revealed a strong positive correlation across all sites. For the whole data set of all stands, a Q10 value of 1.73-2.10 for net C-mineralization and 2.81-3.58 for N2O-N emissions per measured unit was found to describe the temperature dependency of net C-mineralization and N20-N efflux at experimental site. The absence of clear differences between beech and spruce in mono and mixed species cultures on temperature dependencies of net C-mineralization and N2O-N emission rates indicated that the flux rates were not affected by species-specific differences of litter quality.展开更多
A projected skill is adopted by use of the differential evolution (DE) algorithm to calculate a conditional nonlinear optimal perturbation (CNOP). The CNOP is the maximal value of a constrained optimization problem wi...A projected skill is adopted by use of the differential evolution (DE) algorithm to calculate a conditional nonlinear optimal perturbation (CNOP). The CNOP is the maximal value of a constrained optimization problem with a constraint condition, such as a ball constraint. The success of the DE algorithm lies in its ability to handle a non-differentiable and nonlinear cost function. In this study, the DE algorithm and the traditional optimization algorithms used to obtain the CNOPs are compared by analyzing a theoretical grassland ecosystem model and a dynamic global vegetation model. This study shows that the CNOPs generated by the DE algorithm are similar to those by the sequential quadratic programming (SQP) algorithm and the spectral projected gradients (SPG2) algorithm. If the cost function is non-differentiable, the CNOPs could also be caught with the DE algorithm. The numerical results suggest the DE algorithm can be employed to calculate the CNOP, especially when the cost function is non-differentiable.展开更多
The regeneration of its cells is one of the two goals of the plant. The control systems try to allow to cells to optimize their state depending on the circumstances, sometimes in advance. The first of these systems is...The regeneration of its cells is one of the two goals of the plant. The control systems try to allow to cells to optimize their state depending on the circumstances, sometimes in advance. The first of these systems is located in the root. It controls the materialistic input for the regeneration and at the same time is the source of primary information about the state of this input and its development at time. Another element of the control is the system controlling the formation and the distribution of the energy. This control is made without making provision for the state of individual cells. The successfulness of individual cells is taking into account by the system connected with companion cells in the vascular bundle. The similar holds for the second system. It, on the basis of the tension on the boundary of the plant, controls the dynamics of the boundary and removes the tension. The second goal of the control, besides the regeneration, is the optimization of the existence of the plant as whole. For this sake new global criteria arise and are used throughout the control.展开更多
A new efficient adapting virtual intermediate instruction set,V-IIS,is designed and implemented towards the optimized dynamic binary translator (DBT) system.With the help of this powerful but previously little-studied...A new efficient adapting virtual intermediate instruction set,V-IIS,is designed and implemented towards the optimized dynamic binary translator (DBT) system.With the help of this powerful but previously little-studied component,DBTs can not only get rid of the dependence of machine(s),but also get better performance.From our systematical study and evaluation,experimental results demonstrate that if V-IIS is well designed,without affecting the other optimizing measures,this could make DBT's performance close to those who do not have intermediate instructions.This study is an important step towards the grand goal of high performance "multi-source" and "multi-target" dynamic binary translation.展开更多
Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution chara...Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.展开更多
文摘A rational equation of state of the perturbation type with a repulsion and attraction term has been applied to reproduce critical curves of six different binary systems up to high temperatures and pressures. A square well potential for intermolecular interaction is used. With pairwise combination rules for these potentials three adjustable parameters are needed. The experimental critical point and phase equilibrium data are compared with the values predicted using the equation of state. Good agreement is obtained for the analysis of the critical pressure composition data and molar volumes.
基金Hundred Scientists" Project of Ch inese Academy of Sciences.
文摘Aboveground vertical profiles of N2O concentrations were measured with in two natural coniferous-deciduous mixed forests of 1998 and 1999 in Changbai M ountain. Significant high N2O concentrations were found in six profiles out of t welve profiles. The results showed that high concentrations were 3.03% to 64.9% higher than the "normal concentrations" in these six profiles. Differences betwe en the high concentrations and the "normal concentrations" were statistically si gnificant. The simultaneous occurrence of high concentrations at/nearby the cano py height and normal concentrations at the trunk space height indicated an efflu x of N2O from foliage to atmosphere. This study afforded evidence supporting tha t plant per se, besides forest soil, was an important source of atmospheric N2O in a forest ecosystem.
基金Under the auspices of Science and Technology Service Network Initiative Project of the Chinese Academy of Sciences(No.KFJ-EW-STS-002)
文摘Identifying the driving forces that cause changes in forest ecosystem services related to water conservation is essential for the design of interventions that could enhance positive impacts as well as minimizing negative impacts. In this study, we propose an assessment concept framework model for indirect-direct-ecosystem service (IN-DI-ESS) driving forces within this context and method for index construction that considers the selection of a robust and parsimonious variable set. Factor analysis was integrated into two-stage data envelopment analysis (TS-DEA) to determine the driving forces and their effects on water conservation services in forest ecosystems at the provincial scale in China. The results showed the following. 1) Ten indicators with factor scores more than 0.8 were selected as the minimum data set. Four indicators comprising population density, per capita gross domestic product, irrigation efficiency, and per capita food consumption were the indirect driving factors, and six indicators comprising precipitation, farmland into forestry or pasture, forest cover, habitat area, water footprint, and wood extraction were the direct driving forces. 2) Spearman's rank correlation test was performed to compare the overall effectiveness in two periods: stage 1 and stage 2. The calculated coefficients were 0.245, 0.136, and 0.579, respectively, whereas the tabulated value was 0.562. This indicates that the driving forces obviously differed in terms of their contribution to the overall effectiveness and they caused changes in water conservation services in different stages. In terms of the variations in different driving force effects in the years 2000 and 2010, the overall, stage 1, and stage 2 variances were 0.020, 0.065, and 0.079 in 2000, respectively, and 0.018, 0.063, and 0.071 in 2010. This also indicates that heterogeneous driving force effects were obvious in the process during the same period. Identifying the driving forces that affect service changes and evaluating their efficiency have significant policy implications for the management of forest ecosystem services. Advanced effectiveness measures for weak regions could be improved in an appropriate manner. In this study, we showed that factor analysis coupled with TS-DEA based on the IN-D1-ESS framework can increase the parsimony of driving force indicators, as well as interpreting the interactions among indirect and direct driving forces with forest ecosystem water conservation services, and reducing the uncertainty related to the internal consistency during data selection.
基金Project supported by the German Ministry of Consumer Protection, Food and Agriculture (BMVEL) and the German Science Foundation (DFG) (No.WE 1839/1-1)
文摘Mostly based on assumptions derived from controlled-environment studies, predicted future atmospheric CO2 concentrations [CO2] are expected to have considerable impacts on carbon (C) turnover in agro-ecosystems. In order to allow the in situ examination of C-transformations in the plant-soil system of arable crop rotations under future [002], a free air carbon dioxide enrichment (FACE) experiment (550 μmol mol^-1 CO2) was started at Braunschweig, Germany in 1999. The crop rotation under investigation comprised winter barley, a cover crop (ryegrass), sugar beets and winter wheat. Assessments of CO2 effects included the determination of above- and belowground biomass production, measurements of canopy CO2- and H2O- fluxes, soil microbial biomass and in situ soil respiration. The results obtained during the 1st crop rotation cycle (3 years) showed that for the selected crops elevated [CO2] entailed significant positive effects (P 〈 0.05) on aboveground (6%-14% stimulation) and belowground biomass production (up to 90% stimulation), while canopy evapotranspiration was reduced. This resulted in increased soil water content. Also, depending on crop type and season, high CO2 stimulated in situ soil respiration (up to 30%), while soil microbial biomass did not show significant respouses to elevated [CO2] during the first rotation cycle.
基金Under the auspices of International Science and Technology Cooperation Project(No.2010DFA22480)Major State Basic Research Development Program of China(No.2010CB833503)
文摘It is critical to study how different forest management practices affect forest carbon sequestration under global climate change regime. Previous researches focused on the stand-level forest carbon sequestration with rare investigation of forest carbon stocks influ- enced by forest management practices and climate change at regional scale. In this study, a general integrative approach was used to simulate spatial and temporal variations of woody biomass and harvested biomass of forest in China during the 21st century under dif- ferent scenarios of climate and CO2 concentration changes and management tasks by coupling Integrated Terrestrial Ecosystem Carbon budget (InTEC) model with Global Forest Model (G4M). The results showed that forest management practices have more predominant effects on forest stem stocking biomass than climate and CO2 concentration change. Meanwhile, the concurrent future changes in cli- mate and CO2 concentration will enhance the amounts of stem stocking biomass in forests of China by 12%-23% during 2001-2100 relative to that with climate change only. The task for maximizing stem stocking biomass will dramatically enhance the stem stocking biomass from 2001~100, while the task for maximum average increment will result in an increment of stem stocking biomass before 2050 then decline. The difference of woody biomass responding to forest management tasks was owing to the current age structure of forests in China. Meanwhile, the sensitivity of long-term woody biomass to management practices for different forest types (coniferous forest, mixed forest and deciduous forest) under changing climate and CO2 concentration was also analyzed. In addition, longer rotation length under future climate change and rising CO2 concentration scenario will dramatically increase the woody biomass of China during 2001~100. Therefore, our estimation indicated that taking the role of forest management in the carbon cycle into the consideration at regional or national level is very important to project the forest carbon sequestration under future climate change and rising atmospheric CO2 concentration.
基金Project supported by the National Natural Science Foundation of China(Nos.40321101 and 40071036)the Major State Basic Research Development Program of China(973 Program)(No.2002CB412503)
文摘Soil organic carbon(SOC)can act as a sink or source of atmospheric carbon dioxide;therefore,it is important to understand the amount and composition of SOC in terrestrial ecosystems,the spatial variation in SOC,and the underlying mechanisms that stabilize SOC.In this study,density fractionation and acid hydrolysis were used to assess the spatial variation in SOC,the heavy fraction of organic carbon(HFOC),and the resistant organic carbon(ROC)in soils of the southern Hulun Buir region,northeastern China,and to identify the major factors that contribute to this variation.The results showed that as the contents of clay and silt particles(0–50μm)increased,both methylene blue(MB)adsorption by soil minerals and microaggregate contents increased in the 0–20 and 20–40 cm soil layers(P<0.05).Although varying with vegetation types,SOC,HFOC,and ROC contents increased significantly with the content of clay and silt particles, MB adsorption by soil minerals,and microaggregate content(P<0.05),suggesting that soil texture,the MB adsorption by soil minerals,and microaggregate abundance might be important factors influencing the spatial heterogeneity of carbon contents in soils of the southern Hulun Buir region.
文摘The measurement of CO2 and N2O effiux from forest soils is of great importance in evaluating the role of forests as sequestering agents of atmospheric CO2 and nitrogen. To quantify the effect of site on temperature dependence of net C-mineralization and N2O-N emissions, three adjacent forest floors under beech, Norway spruce and mixed species stands were investigated at Soiling forest, Germany, by an incubation experiment for three months. The investigated net C-mineralization and N2O-N emissions from all forest floors exhibited an exponential increase with respect to temperature elevation. The temperature coefficient function (Q10 value), was fitted to flux rates to describe the temperature sensitivity of forest floors on temperature in the range of 1-20℃. Comparing the fitted curves for temperature sensitivity of the forest floors in relation to net carbon mineralization and nitrous oxide emission rates revealed a strong positive correlation across all sites. For the whole data set of all stands, a Q10 value of 1.73-2.10 for net C-mineralization and 2.81-3.58 for N2O-N emissions per measured unit was found to describe the temperature dependency of net C-mineralization and N20-N efflux at experimental site. The absence of clear differences between beech and spruce in mono and mixed species cultures on temperature dependencies of net C-mineralization and N2O-N emission rates indicated that the flux rates were not affected by species-specific differences of litter quality.
基金provided by grants from the National Basic Research Program of China (Grant No. 2006CB400503)LASG Free Exploration Fund+1 种基金LASG State Key Laboratory Special Fundthe KZCX3-SW-230 of the Chinese Academy of Sciences
文摘A projected skill is adopted by use of the differential evolution (DE) algorithm to calculate a conditional nonlinear optimal perturbation (CNOP). The CNOP is the maximal value of a constrained optimization problem with a constraint condition, such as a ball constraint. The success of the DE algorithm lies in its ability to handle a non-differentiable and nonlinear cost function. In this study, the DE algorithm and the traditional optimization algorithms used to obtain the CNOPs are compared by analyzing a theoretical grassland ecosystem model and a dynamic global vegetation model. This study shows that the CNOPs generated by the DE algorithm are similar to those by the sequential quadratic programming (SQP) algorithm and the spectral projected gradients (SPG2) algorithm. If the cost function is non-differentiable, the CNOPs could also be caught with the DE algorithm. The numerical results suggest the DE algorithm can be employed to calculate the CNOP, especially when the cost function is non-differentiable.
文摘The regeneration of its cells is one of the two goals of the plant. The control systems try to allow to cells to optimize their state depending on the circumstances, sometimes in advance. The first of these systems is located in the root. It controls the materialistic input for the regeneration and at the same time is the source of primary information about the state of this input and its development at time. Another element of the control is the system controlling the formation and the distribution of the energy. This control is made without making provision for the state of individual cells. The successfulness of individual cells is taking into account by the system connected with companion cells in the vascular bundle. The similar holds for the second system. It, on the basis of the tension on the boundary of the plant, controls the dynamics of the boundary and removes the tension. The second goal of the control, besides the regeneration, is the optimization of the existence of the plant as whole. For this sake new global criteria arise and are used throughout the control.
基金Projects(12R21414600)supported by Shanghai Municipal Science and Technology Commission,China
文摘A new efficient adapting virtual intermediate instruction set,V-IIS,is designed and implemented towards the optimized dynamic binary translator (DBT) system.With the help of this powerful but previously little-studied component,DBTs can not only get rid of the dependence of machine(s),but also get better performance.From our systematical study and evaluation,experimental results demonstrate that if V-IIS is well designed,without affecting the other optimizing measures,this could make DBT's performance close to those who do not have intermediate instructions.This study is an important step towards the grand goal of high performance "multi-source" and "multi-target" dynamic binary translation.
基金Under the auspices of Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-223)National Natural Science Foundation of China (No. 40803023)+1 种基金Key Program of Natural Science Foundation of Shandong Province(No. ZR2010DZ001)Talents Foundation of Chinese Academy of Sciences (No. AJ0809BX-036)
文摘Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.