目的探讨二氯二苯基三氯乙烷(dichlorodiphenyltrichloroethane,DDT)诱导人结直肠癌增殖、侵袭与微小RNA-129(microRNA-129,miR-129)/细胞周期蛋白依赖性激酶-14(cyclin-dependent kinase-14,CDK-14)表达的关系。方法5×106个/m L...目的探讨二氯二苯基三氯乙烷(dichlorodiphenyltrichloroethane,DDT)诱导人结直肠癌增殖、侵袭与微小RNA-129(microRNA-129,miR-129)/细胞周期蛋白依赖性激酶-14(cyclin-dependent kinase-14,CDK-14)表达的关系。方法5×106个/m L密度的人结直肠癌细胞分别用终浓度为0、100、250和500 nmol/L的DDT处理72 h,运用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]法及甲基紫染色测定细胞活力的和细胞细胞集落,流式细胞仪测定细胞凋亡水平,Transwell小室(transwell chamber,Transwell)侵袭室及划痕试验测定细胞侵袭迁移水平,实时荧光逆转录(real-time fluorescent reverse transcription,RT-PCR)法及蛋白印迹法测定细胞miR-129、CDK-14、卷曲螺旋结构域-34(coiled-coil domain-34,CCDC34)、半乳糖激酶1(Galactokinase 1,GALK1)水平。结果100、250和500 nmol/L DDT组细胞增殖水平[(70.59±7.72、76.63±8.32、84.25±9.04 vs 62.58±6.83)%]、细胞集落[(99.95±9.58、215.63±19.85、398.96±35.26 vs 62.63±6.52)个]、侵袭数目[(76.25±9.58、154.62±19.51、298.96±38.57 vs 49.65±6.65)个]、迁移水平(75.96±14.58、119.69±20.59、165.63±32.68 vs 11.59±2.28)、CDK-14、CCDC34、GALK1 m RNA蛋白水平明显高于对照组(P<0.01),凋亡率、miR-129水平明显低于对照组(P<0.01);且500 nmol/L DDT组细胞增殖水平、细胞集落、侵袭数目、迁移水平、CDK-14 mRNA蛋白[(1.60±0.28、2.14±0.33、2.99±0.51 vs 0.92±0.16),(0.42±0.07、0.85±0.14、1.20±0.20 vs 0.21±0.03)]、CCDC34(0.48±0.08、0.87±0.15、1.18±0.18 vs 0.28±0.07)、GALK1(0.50±0.09、0.84±0.14、1.22±0.19 vs 0.30±0.08)蛋白水平均高于250 nmol/L DDT组,差异均有统计学意义(均P<0.01),凋亡率(1.70±0.31、1.00±0.16、0.60±0.11 vs 3.00±0.51)、miR-129(1.42±0.24、0.92±0.15、0.64±0.10 vs 1.78±0.28)水平均低于250 nmol/L DDT组,差异均有统计学意义(均P<0.01);250nmol/L DDT组细胞增殖水平、细胞集落、侵袭数目、迁移水平、CDK-14、CCDC34、GALK1 mRNA蛋白水平均高于100 nmol/L DDT组,差异均有统计学意义(均P<0.01),250 nmol/L DDT组细胞凋亡率、miR-129水平均低于100 nmol/L DDT组,差异均有统计学意义(均P<0.01);DDT浓度与细胞增殖水平、细胞集落、侵袭数目、迁移水平、凋亡率、miR-129水平、CDK-14、CCDC34、GALK1 mRNA蛋白水平有明显剂量-反应关系(P<0.01)。结论DDT下调miR-129表达靶向上调调控CDK-14以及CCDC34、GALK1的表达,从而促进结直肠癌细胞的增殖和侵袭,并抑制其凋亡。展开更多
Sixteen surface sediment samples were collected and analysed to evaluate the residues of organochlorine pesticides(OCPs) from intertidal flat in Jiangsu Province. Overall, 22 OCPs were detected with total concentratio...Sixteen surface sediment samples were collected and analysed to evaluate the residues of organochlorine pesticides(OCPs) from intertidal flat in Jiangsu Province. Overall, 22 OCPs were detected with total concentrations of OCPs ranging widely from 0.96 to 12.14 ng/g(dry wt). Total hexachlorocyclohexane(HCH) and total dichlorodiphenyltrichloroethane(DDT) levels varied from <0.01 to 0.67 ng/g and from 0.23 to 4.85 ng/g, respectively. DDTs were the predominant compounds. The dominance of β-HCH indicated a history of HCH pollution. According to the ratios of( p, p'-DDD+ p, p'-DDE)/ p, p '-DDT and o, p ′-DDT/ p, p ′-DDT, new input of DDTs did not occur in most sites, and the main sources were historical usage of technical DDTs. OCPs such as dieldrin, endrin, p, p ′-DDD, and p, p ′-DDT exceeded the effects range low, showing adverse biological effects that would occasionally occur at some sites of the study area.展开更多
The concentrations of 28 organochlorine pesticides(OCPs)in the urban air of Dalian,China were measured using semipermeable membrane devices(SPMDs)for the first time.Alpha-hexachlorocyclohexane(a-HCH),b-HCH,pentachloro...The concentrations of 28 organochlorine pesticides(OCPs)in the urban air of Dalian,China were measured using semipermeable membrane devices(SPMDs)for the first time.Alpha-hexachlorocyclohexane(a-HCH),b-HCH,pentachlorobenzene(PentCB),hexachlorobenzene(HexCB),4,40-dichlorodiphenyltrichloroethane(4,40-DDT),4,40-dichlorodiphenyldichloroethene,endosulfan-I and endosulfan-II were the predominant OCPs in the urban air of Dalian.The concentration of OCPs sequestrated by SPMDs(CSPMD)ranged from undetected to 56,772 pg SPMD-1and had a linear correlation with the reciprocal of the average ambient atmospheric temperature(1/T).The CSPMDof PentCB and HexCB had negative correlations with T,whereas the other detected OCPs had a positive correlation.The source of HCHs,DDTs,HexCB,PentCB,chlordane,and endosulfan in the air were also evaluated.The OCPs in the urban air of Dalian could be attributed to the seasonal usage of pesticides,combustion,volatilization of aged OCPs from soil,water,plants,and long-range atmospheric transport.展开更多
文摘目的探讨二氯二苯基三氯乙烷(dichlorodiphenyltrichloroethane,DDT)诱导人结直肠癌增殖、侵袭与微小RNA-129(microRNA-129,miR-129)/细胞周期蛋白依赖性激酶-14(cyclin-dependent kinase-14,CDK-14)表达的关系。方法5×106个/m L密度的人结直肠癌细胞分别用终浓度为0、100、250和500 nmol/L的DDT处理72 h,运用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]法及甲基紫染色测定细胞活力的和细胞细胞集落,流式细胞仪测定细胞凋亡水平,Transwell小室(transwell chamber,Transwell)侵袭室及划痕试验测定细胞侵袭迁移水平,实时荧光逆转录(real-time fluorescent reverse transcription,RT-PCR)法及蛋白印迹法测定细胞miR-129、CDK-14、卷曲螺旋结构域-34(coiled-coil domain-34,CCDC34)、半乳糖激酶1(Galactokinase 1,GALK1)水平。结果100、250和500 nmol/L DDT组细胞增殖水平[(70.59±7.72、76.63±8.32、84.25±9.04 vs 62.58±6.83)%]、细胞集落[(99.95±9.58、215.63±19.85、398.96±35.26 vs 62.63±6.52)个]、侵袭数目[(76.25±9.58、154.62±19.51、298.96±38.57 vs 49.65±6.65)个]、迁移水平(75.96±14.58、119.69±20.59、165.63±32.68 vs 11.59±2.28)、CDK-14、CCDC34、GALK1 m RNA蛋白水平明显高于对照组(P<0.01),凋亡率、miR-129水平明显低于对照组(P<0.01);且500 nmol/L DDT组细胞增殖水平、细胞集落、侵袭数目、迁移水平、CDK-14 mRNA蛋白[(1.60±0.28、2.14±0.33、2.99±0.51 vs 0.92±0.16),(0.42±0.07、0.85±0.14、1.20±0.20 vs 0.21±0.03)]、CCDC34(0.48±0.08、0.87±0.15、1.18±0.18 vs 0.28±0.07)、GALK1(0.50±0.09、0.84±0.14、1.22±0.19 vs 0.30±0.08)蛋白水平均高于250 nmol/L DDT组,差异均有统计学意义(均P<0.01),凋亡率(1.70±0.31、1.00±0.16、0.60±0.11 vs 3.00±0.51)、miR-129(1.42±0.24、0.92±0.15、0.64±0.10 vs 1.78±0.28)水平均低于250 nmol/L DDT组,差异均有统计学意义(均P<0.01);250nmol/L DDT组细胞增殖水平、细胞集落、侵袭数目、迁移水平、CDK-14、CCDC34、GALK1 mRNA蛋白水平均高于100 nmol/L DDT组,差异均有统计学意义(均P<0.01),250 nmol/L DDT组细胞凋亡率、miR-129水平均低于100 nmol/L DDT组,差异均有统计学意义(均P<0.01);DDT浓度与细胞增殖水平、细胞集落、侵袭数目、迁移水平、凋亡率、miR-129水平、CDK-14、CCDC34、GALK1 mRNA蛋白水平有明显剂量-反应关系(P<0.01)。结论DDT下调miR-129表达靶向上调调控CDK-14以及CCDC34、GALK1的表达,从而促进结直肠癌细胞的增殖和侵袭,并抑制其凋亡。
基金Supported by the National Natural Science Foundation of China(Nos.40871216,41271466)
文摘Sixteen surface sediment samples were collected and analysed to evaluate the residues of organochlorine pesticides(OCPs) from intertidal flat in Jiangsu Province. Overall, 22 OCPs were detected with total concentrations of OCPs ranging widely from 0.96 to 12.14 ng/g(dry wt). Total hexachlorocyclohexane(HCH) and total dichlorodiphenyltrichloroethane(DDT) levels varied from <0.01 to 0.67 ng/g and from 0.23 to 4.85 ng/g, respectively. DDTs were the predominant compounds. The dominance of β-HCH indicated a history of HCH pollution. According to the ratios of( p, p'-DDD+ p, p'-DDE)/ p, p '-DDT and o, p ′-DDT/ p, p ′-DDT, new input of DDTs did not occur in most sites, and the main sources were historical usage of technical DDTs. OCPs such as dieldrin, endrin, p, p ′-DDD, and p, p ′-DDT exceeded the effects range low, showing adverse biological effects that would occasionally occur at some sites of the study area.
基金supported by the National Natural Science Foundation of China (20877011)the DAAD (German Academic Exchange Service) Scientific Research Visiting Scholarship and the National High Technology Research and Development Programs of China (2011AA060604)
文摘The concentrations of 28 organochlorine pesticides(OCPs)in the urban air of Dalian,China were measured using semipermeable membrane devices(SPMDs)for the first time.Alpha-hexachlorocyclohexane(a-HCH),b-HCH,pentachlorobenzene(PentCB),hexachlorobenzene(HexCB),4,40-dichlorodiphenyltrichloroethane(4,40-DDT),4,40-dichlorodiphenyldichloroethene,endosulfan-I and endosulfan-II were the predominant OCPs in the urban air of Dalian.The concentration of OCPs sequestrated by SPMDs(CSPMD)ranged from undetected to 56,772 pg SPMD-1and had a linear correlation with the reciprocal of the average ambient atmospheric temperature(1/T).The CSPMDof PentCB and HexCB had negative correlations with T,whereas the other detected OCPs had a positive correlation.The source of HCHs,DDTs,HexCB,PentCB,chlordane,and endosulfan in the air were also evaluated.The OCPs in the urban air of Dalian could be attributed to the seasonal usage of pesticides,combustion,volatilization of aged OCPs from soil,water,plants,and long-range atmospheric transport.
