In this study,porous silica with high surface area was prepared through selective leaching of thermally activated chlorite in HCl solution.In the process,chlorite was activated by pre-calcining treatment,then activate...In this study,porous silica with high surface area was prepared through selective leaching of thermally activated chlorite in HCl solution.In the process,chlorite was activated by pre-calcining treatment,then activated components(MgO,Al_(2)O_(3),and Fe_(2)O_(3))were selectively leached by acid solution,resulting in the formation of nanopores in situ.The morphology,structure,surface area and pore-size distribution of the material were characterized by XRD,TG/DSC,^(27)Al MAS NMR,SEM,TEM and N2 adsorption−desorption isotherms.The highest specific surface area(SBET=333 m^(2)/g)was obtained by selectively leaching the 600℃ calcined chlorite from 3 mol/L HCl at 90℃ for 2 h.The pore sizes and specific surface areas can be controlled by calcination and leaching conditions.The ^(27)Al MAS NMR spectra of the samples revealed the relationship between structural transformation and the selective acid leaching properties of thermal-activated chlorite,demonstrating that AlVI transfers into AlV when chlorite changes into activated chlorite during thermal activation,and the coordinations of Al has a significant effect on acid solubility of chlorite.The as-prepared porous silica showed favorable adsorption abilities with capacity of 148.79 mg/g for methylene blue at pH of about 7 and temperature of 25℃,indicating its promising potential in adsorption application.展开更多
The side-glowing optical fibers (SOFs) were chosen as the conducting medium of endogenous light; and 20 mg·L-1 methylene blue was chosen as the target to be degraded. The SOF is made up of quartz core with a sili...The side-glowing optical fibers (SOFs) were chosen as the conducting medium of endogenous light; and 20 mg·L-1 methylene blue was chosen as the target to be degraded. The SOF is made up of quartz core with a silicon cladding, which can emit light through side surface more uniformly and transmit light for longer distance to avoid attenuation of light by liquid medium. The filament lamp was chosen as visible light source. Different reaction conditions, such as the presence of optical fiber or not, the quantity of SOF, light irradiation intensity were tested by measuring the methylene blue degradation of methylene blue. The results show that suitable reaction conditions were 1.167 g·L-1 Ag + /TiO 2 with 7% (by mass) of Ag + doped in TiO 2 , and 500 roots of SOF (30 cm length in solution). The photocatalytic degradation efficiency under 300W lamp irradiation for 8h was about 97%. And the photocatalytic degradation efficiency of methylene blue degradation was proportional to SOF quantity, light irradiation intensity and catalytic dosage within a certain range. Compared with general UV and visible light SOFs could save a huge amount of energy and cost, in the potential applications in dealing with organic pollutants on a large scale.展开更多
OBJECTIVE: To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro. METHODS: IEC-6 cells were seeded in 96-well microplates at various densit...OBJECTIVE: To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro. METHODS: IEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm. RESULTS: There was a positive relationship between cell number and OD value (r = 0.954, P 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3. CONCLUSION: Proliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.展开更多
To characterize the role of plating densities and alpha difluoromethylornithine (DFMO) on the proliferation of IEC 6 cells in vitro Methods IEC 6 cells were seeded in 96 well microplates at various densitie...To characterize the role of plating densities and alpha difluoromethylornithine (DFMO) on the proliferation of IEC 6 cells in vitro Methods IEC 6 cells were seeded in 96 well microplates at various densities in the pre sence or absence of DFMO Cells were counted and their proliferative capabilit y was monitored Days 1 to 7 with MTT assay at an optical density of 570?nm Results There was a positive relationship between cell number and OD value ( r =0 954 , P 【0 01) Higher plating densities (】0 5×10 4 cells/well) inhibited the growth of cells on Day 2 When the density reaches 4×10 4 cells/well, the O D value increased gradually and reached a peak on Day 5 After that, the OD va lue began to fall The growth of IEC 6 cells was limited at a low density (0 2×10 4 cells/well) on Day 4 DFMO caused a complete inhibition of proliferati on of IEC 6 cells on Days 1 to 3 Conclusion Proliferation of IEC 6 cells is related to plating density and incubation time It is inhibited by DFMO, but is reversible when the incubation time is prolon ged展开更多
基金Project(51772153)supported by the National Natural Science Foundation of China。
文摘In this study,porous silica with high surface area was prepared through selective leaching of thermally activated chlorite in HCl solution.In the process,chlorite was activated by pre-calcining treatment,then activated components(MgO,Al_(2)O_(3),and Fe_(2)O_(3))were selectively leached by acid solution,resulting in the formation of nanopores in situ.The morphology,structure,surface area and pore-size distribution of the material were characterized by XRD,TG/DSC,^(27)Al MAS NMR,SEM,TEM and N2 adsorption−desorption isotherms.The highest specific surface area(SBET=333 m^(2)/g)was obtained by selectively leaching the 600℃ calcined chlorite from 3 mol/L HCl at 90℃ for 2 h.The pore sizes and specific surface areas can be controlled by calcination and leaching conditions.The ^(27)Al MAS NMR spectra of the samples revealed the relationship between structural transformation and the selective acid leaching properties of thermal-activated chlorite,demonstrating that AlVI transfers into AlV when chlorite changes into activated chlorite during thermal activation,and the coordinations of Al has a significant effect on acid solubility of chlorite.The as-prepared porous silica showed favorable adsorption abilities with capacity of 148.79 mg/g for methylene blue at pH of about 7 and temperature of 25℃,indicating its promising potential in adsorption application.
文摘The side-glowing optical fibers (SOFs) were chosen as the conducting medium of endogenous light; and 20 mg·L-1 methylene blue was chosen as the target to be degraded. The SOF is made up of quartz core with a silicon cladding, which can emit light through side surface more uniformly and transmit light for longer distance to avoid attenuation of light by liquid medium. The filament lamp was chosen as visible light source. Different reaction conditions, such as the presence of optical fiber or not, the quantity of SOF, light irradiation intensity were tested by measuring the methylene blue degradation of methylene blue. The results show that suitable reaction conditions were 1.167 g·L-1 Ag + /TiO 2 with 7% (by mass) of Ag + doped in TiO 2 , and 500 roots of SOF (30 cm length in solution). The photocatalytic degradation efficiency under 300W lamp irradiation for 8h was about 97%. And the photocatalytic degradation efficiency of methylene blue degradation was proportional to SOF quantity, light irradiation intensity and catalytic dosage within a certain range. Compared with general UV and visible light SOFs could save a huge amount of energy and cost, in the potential applications in dealing with organic pollutants on a large scale.
文摘OBJECTIVE: To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro. METHODS: IEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm. RESULTS: There was a positive relationship between cell number and OD value (r = 0.954, P 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3. CONCLUSION: Proliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.
基金theNationalEmphasisDevelopingProgramofBasicResearch (973 ) (No G19990 5 44 )theNationalNaturalscienceFoundationofChina (No 3 9970 90 6)
文摘To characterize the role of plating densities and alpha difluoromethylornithine (DFMO) on the proliferation of IEC 6 cells in vitro Methods IEC 6 cells were seeded in 96 well microplates at various densities in the pre sence or absence of DFMO Cells were counted and their proliferative capabilit y was monitored Days 1 to 7 with MTT assay at an optical density of 570?nm Results There was a positive relationship between cell number and OD value ( r =0 954 , P 【0 01) Higher plating densities (】0 5×10 4 cells/well) inhibited the growth of cells on Day 2 When the density reaches 4×10 4 cells/well, the O D value increased gradually and reached a peak on Day 5 After that, the OD va lue began to fall The growth of IEC 6 cells was limited at a low density (0 2×10 4 cells/well) on Day 4 DFMO caused a complete inhibition of proliferati on of IEC 6 cells on Days 1 to 3 Conclusion Proliferation of IEC 6 cells is related to plating density and incubation time It is inhibited by DFMO, but is reversible when the incubation time is prolon ged
