Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDN...Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDNSF and nestin. Methods The spinal cord contusion model of rat was established according to Allen＇s falling strike method. The expression of SDNSF was studied by RT-PCR and in situ hybridization （ISH）, and the expression of nestin was detected by immunochemistry. Results RT-PCR revealed that SDNSF mRNA was upregulated on day 4 after injury, peaked on day 8-12, and decreased to the sham operation level on day 16. ISH revealed that SDNSF mRNA was mainly expressed in the gray matter cells, probably neurons, of spinal cord. The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice. However, the distribution pattern of these new cells was different from that of SDNSF-positive cells. Conclusion （1） SDNSF is expressed in the gray matter of spinal cord. The expression of SDNSF mRNA in the spinal cord varies with injured time. （2） The nestin-positive cells proliferate accompanied with spinal cord injury repair, but do not secrete SDNSF.展开更多
Objective This study aimed to design and optimize the water extraction process for Chuantieling(喘贴灵,CTL)based on the concept of quality by design(QbD).Methods The single-factor experiments were used to select the b...Objective This study aimed to design and optimize the water extraction process for Chuantieling(喘贴灵,CTL)based on the concept of quality by design(QbD).Methods The single-factor experiments were used to select the best experimental points of CTL water extraction.On this basis,the transfer rate of ephedrine hydrochloride and sin-apine thiocyanate,and the yield of the extract were evaluated as the evaluation indicators.The liquid-solid ratio,extraction time,and pH value were selected as the main factors to carry out the Box-Behnken design(BBD).A mathematical model of the critical process parameters(CPPs)and critical quality attributes(CQAs)was established,the interaction between CQAs and CPPs was examined,and the CTL extraction process design space was established and optimized,which guaranteed the stability of the process.The high performance liquid chro-matography(HPLC)was used to analyze the main active compounds in the CTL extract.Results Through single-factor experiments,the best experimental parameters were found to be a liquid-solid ratio of 8∶1,extraction time of 90 min,pH value of 4,and extraction times of three.The experimental variance analysis results of the BBD showed that the P value of the re-gression model was less than 0.05,and the lack-of-fit value was greater than 0.01,indicating that the constructed model had good predictive ability.The operating space of the CPPs of the CTL water extraction process was combined with the actual production situation.In the pro-duction situation,the best extraction process was nine times of water addition,72 min of ex-traction time,and 4.5 of pH value.The HPLC results showed that the peak areas of ephedrine hydrochloride,sinapine thiocyanate,tetrahydropalmatine,methyl eugenol,cinnamaldehyde,and 6-gingerol in the CTL extract accounted for 0.94%,14.32%,0.78%,31.23%,0.34%,and 0.44%of the total peak area,respectively.Conclusion The water extraction process design space of CTL based on QbD was conducive to actual production operations,ensuring the stability of the process.展开更多
The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance r...The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance resources. Basing on the analysis of the characteristics and hazards of this population, the writer puts forward the suggestions to construct the urban vagrants＇ accurate rescue system with the relevant suggestions： perfecting the relief systern, fulfilling the basic functions of the relief management station, setting up an environment of sound laws and regulations. Take the temporary solution as the way and the permanent cure as the goal, not only achieve the resolve of the matter on surface, but also the matter of essence, enhance the overall viability of the urban wandering population.展开更多
Social forestry has emerged as a popular approach to achieving landscape justice by empowering local communities.However,the development and implementation of such programs often face challenges.This paper explores th...Social forestry has emerged as a popular approach to achieving landscape justice by empowering local communities.However,the development and implementation of such programs often face challenges.This paper explores the concept of landscape justice within the context of Indonesian social forestry in two ways.First,it juxtaposes the social forestry program with palm oil plantations to highlight the relationship between environmental initiatives and capital expansion,and the formation of green capitalism.By examining the historical development of social forestry,the paper argues that current political and legal frameworks have facilitated the depoliticization of previously radical,anti-capitalist,and anti-palm oil civil movements,despite notionally“empowering”local communities.Second,the paper interrogates the inclusivity of the social forestry program within local communities,noting that NGOs sometimes label local people as“cooperative”or“stubborn,”thus overlooking the pre-existing social tensions.The paper posits that more attention should be given to the social foundations underlying environmental projects and the new eco-social structure arising from environmental governance.展开更多
文摘Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDNSF and nestin. Methods The spinal cord contusion model of rat was established according to Allen＇s falling strike method. The expression of SDNSF was studied by RT-PCR and in situ hybridization （ISH）, and the expression of nestin was detected by immunochemistry. Results RT-PCR revealed that SDNSF mRNA was upregulated on day 4 after injury, peaked on day 8-12, and decreased to the sham operation level on day 16. ISH revealed that SDNSF mRNA was mainly expressed in the gray matter cells, probably neurons, of spinal cord. The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice. However, the distribution pattern of these new cells was different from that of SDNSF-positive cells. Conclusion （1） SDNSF is expressed in the gray matter of spinal cord. The expression of SDNSF mRNA in the spinal cord varies with injured time. （2） The nestin-positive cells proliferate accompanied with spinal cord injury repair, but do not secrete SDNSF.
基金Hunan University of Traditional Chinese Medicine Graduate Training Quality Engineering Project(2019CX57)the First-class Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine (201803)。
文摘Objective This study aimed to design and optimize the water extraction process for Chuantieling(喘贴灵,CTL)based on the concept of quality by design(QbD).Methods The single-factor experiments were used to select the best experimental points of CTL water extraction.On this basis,the transfer rate of ephedrine hydrochloride and sin-apine thiocyanate,and the yield of the extract were evaluated as the evaluation indicators.The liquid-solid ratio,extraction time,and pH value were selected as the main factors to carry out the Box-Behnken design(BBD).A mathematical model of the critical process parameters(CPPs)and critical quality attributes(CQAs)was established,the interaction between CQAs and CPPs was examined,and the CTL extraction process design space was established and optimized,which guaranteed the stability of the process.The high performance liquid chro-matography(HPLC)was used to analyze the main active compounds in the CTL extract.Results Through single-factor experiments,the best experimental parameters were found to be a liquid-solid ratio of 8∶1,extraction time of 90 min,pH value of 4,and extraction times of three.The experimental variance analysis results of the BBD showed that the P value of the re-gression model was less than 0.05,and the lack-of-fit value was greater than 0.01,indicating that the constructed model had good predictive ability.The operating space of the CPPs of the CTL water extraction process was combined with the actual production situation.In the pro-duction situation,the best extraction process was nine times of water addition,72 min of ex-traction time,and 4.5 of pH value.The HPLC results showed that the peak areas of ephedrine hydrochloride,sinapine thiocyanate,tetrahydropalmatine,methyl eugenol,cinnamaldehyde,and 6-gingerol in the CTL extract accounted for 0.94%,14.32%,0.78%,31.23%,0.34%,and 0.44%of the total peak area,respectively.Conclusion The water extraction process design space of CTL based on QbD was conducive to actual production operations,ensuring the stability of the process.
文摘The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance resources. Basing on the analysis of the characteristics and hazards of this population, the writer puts forward the suggestions to construct the urban vagrants＇ accurate rescue system with the relevant suggestions： perfecting the relief systern, fulfilling the basic functions of the relief management station, setting up an environment of sound laws and regulations. Take the temporary solution as the way and the permanent cure as the goal, not only achieve the resolve of the matter on surface, but also the matter of essence, enhance the overall viability of the urban wandering population.
文摘Social forestry has emerged as a popular approach to achieving landscape justice by empowering local communities.However,the development and implementation of such programs often face challenges.This paper explores the concept of landscape justice within the context of Indonesian social forestry in two ways.First,it juxtaposes the social forestry program with palm oil plantations to highlight the relationship between environmental initiatives and capital expansion,and the formation of green capitalism.By examining the historical development of social forestry,the paper argues that current political and legal frameworks have facilitated the depoliticization of previously radical,anti-capitalist,and anti-palm oil civil movements,despite notionally“empowering”local communities.Second,the paper interrogates the inclusivity of the social forestry program within local communities,noting that NGOs sometimes label local people as“cooperative”or“stubborn,”thus overlooking the pre-existing social tensions.The paper posits that more attention should be given to the social foundations underlying environmental projects and the new eco-social structure arising from environmental governance.
