A study was conducted to evaluate the effect of GA3, moist-chilling and temperature on seed germination ofAbies pindrow and Picea smithiana from five different provenances. Seeds were soaked in GA3 （10 mg·L^-1）...A study was conducted to evaluate the effect of GA3, moist-chilling and temperature on seed germination ofAbies pindrow and Picea smithiana from five different provenances. Seeds were soaked in GA3 （10 mg·L^-1） for 24 h, then chilled at 3-5℃ for 15 days. Four temperature regimes viz. 10℃, 15℃, 20℃ and 25℃ were used for stimulating seed germination. Results showed that soaking and chilling significantly increased germination percentage. The germination percentage was highest at 10℃. Overall results showed that soaking seeds in GA3 （10 mg·L^-1） for 24 h, moist chilling for 15 days, and germinating at 10℃ produced an effective germination in both the species studied. The statistical analysis of the data proclaimed significant effect of treatment, temperature, provenance and treatment with temperature interactions on seed germination.展开更多
The aim of this study was to determine the volatile composition of essential oil of four Picea Mill. species （Piceapungens Engelm., Picea mariana （Mill.） Britton, Picea glauca （Moench） Voss., Picea rubens Sarg.）...The aim of this study was to determine the volatile composition of essential oil of four Picea Mill. species （Piceapungens Engelm., Picea mariana （Mill.） Britton, Picea glauca （Moench） Voss., Picea rubens Sarg.） needles. The volatile components extracted from these four species needles were analyzed by using headspace solid phase microextraction （HS-SPME）/gas chromatography-mass spectrum （GC-MS） and 31, 34, 27 and 24 compounds were identified representing 91.77%, 92.70% 92.38% and 94.06% of the total oil, respectively. The major constituents were found to be bornylacetate （29.40%）, camphor （26.43%）, 13-myrcene （7.47%） and camphene （7.01%） in P. pungens; camphene （22.03%）, bornylacetate （21.64%）, α-pinene （16.62%） and borneol （7.79%） in P. mariana; bornylacetate （31.25%）, limonene （17.27%）, α-pinene （15.85%）; camphene （13.65%） in P. glauca and borneol （12.38%）, α-pinene （10.36%）, germacrene D （9.86%） and δ-cadinene （8.25%） in P. rubens. This study sought to detecte new phytochemical data on the Picea genus to help chemotaxonomy and usable of studied species.展开更多
Aims Black spruce(Picea mariana[Mill.]B.S.P.)and white spruce(Picea glauca[Moench]Voss.)are congeneric species.Both are moderately shade tolerant and widely distributed across North American boreal forests.Methods To ...Aims Black spruce(Picea mariana[Mill.]B.S.P.)and white spruce(Picea glauca[Moench]Voss.)are congeneric species.Both are moderately shade tolerant and widely distributed across North American boreal forests.Methods To understand light effects on their ecophysiological responses to elevated CO_(2),1-year-old seedlings were exposed to 360µmol mol−1 and 720µmol mol−1 CO_(2)at three light conditions(100%,50%and 30%of full light in the greenhouse).Foliar gas exchanges were measured in the mid-and late-growing season.Important Findings Elevated CO_(2)increased net photosynthesis(Pn)and photosynthetic water use efficiency,but it reduced stomatal conductance and transpiration.The stimulation of photosynthesis by elevated CO_(2)was greatest at 50%light and smallest at 100%.Photosynthesis,maximum carboxylation rate(Vcmax)and light-saturated rate of electron transport(Jmax)all decreased with decreasing light.Elevated CO_(2)significantly reduced Vcmax across all light treatments and both species in mid-growing season.However,the effect of elevated CO_(2)became insignificant at 30%light later in the growing season,with the response being greater in black spruce than in white spruce.Elevated CO_(2)also reduced Jmax in white spruce in both measurements while the effect became insignificant at 30%light later in the growing season.However,the effect on black spruce varied with time.Elevated CO_(2)reduced Jmax in black spruce in mid-growing season in all light treatments and the effect became insignificant at 30%light later in the growing season,while it increased Jmax later in the season at 100%and 50%light.These results suggest that both species benefited from elevated CO_(2),and that the responses varied with light supply,such that the response was primarily physiological at 100%and 50%light,while it was primarily morphological at 30%light.展开更多
文摘A study was conducted to evaluate the effect of GA3, moist-chilling and temperature on seed germination ofAbies pindrow and Picea smithiana from five different provenances. Seeds were soaked in GA3 （10 mg·L^-1） for 24 h, then chilled at 3-5℃ for 15 days. Four temperature regimes viz. 10℃, 15℃, 20℃ and 25℃ were used for stimulating seed germination. Results showed that soaking and chilling significantly increased germination percentage. The germination percentage was highest at 10℃. Overall results showed that soaking seeds in GA3 （10 mg·L^-1） for 24 h, moist chilling for 15 days, and germinating at 10℃ produced an effective germination in both the species studied. The statistical analysis of the data proclaimed significant effect of treatment, temperature, provenance and treatment with temperature interactions on seed germination.
文摘The aim of this study was to determine the volatile composition of essential oil of four Picea Mill. species （Piceapungens Engelm., Picea mariana （Mill.） Britton, Picea glauca （Moench） Voss., Picea rubens Sarg.） needles. The volatile components extracted from these four species needles were analyzed by using headspace solid phase microextraction （HS-SPME）/gas chromatography-mass spectrum （GC-MS） and 31, 34, 27 and 24 compounds were identified representing 91.77%, 92.70% 92.38% and 94.06% of the total oil, respectively. The major constituents were found to be bornylacetate （29.40%）, camphor （26.43%）, 13-myrcene （7.47%） and camphene （7.01%） in P. pungens; camphene （22.03%）, bornylacetate （21.64%）, α-pinene （16.62%） and borneol （7.79%） in P. mariana; bornylacetate （31.25%）, limonene （17.27%）, α-pinene （15.85%）; camphene （13.65%） in P. glauca and borneol （12.38%）, α-pinene （10.36%）, germacrene D （9.86%） and δ-cadinene （8.25%） in P. rubens. This study sought to detecte new phytochemical data on the Picea genus to help chemotaxonomy and usable of studied species.
基金by Natural Sciences and Engineering Research Council of Canada Discovery grants to Q.L.Dang(Project No.203198-2013-RGPIN)Lakehead University Graduate Assistantships to J.Marfo.
文摘Aims Black spruce(Picea mariana[Mill.]B.S.P.)and white spruce(Picea glauca[Moench]Voss.)are congeneric species.Both are moderately shade tolerant and widely distributed across North American boreal forests.Methods To understand light effects on their ecophysiological responses to elevated CO_(2),1-year-old seedlings were exposed to 360µmol mol−1 and 720µmol mol−1 CO_(2)at three light conditions(100%,50%and 30%of full light in the greenhouse).Foliar gas exchanges were measured in the mid-and late-growing season.Important Findings Elevated CO_(2)increased net photosynthesis(Pn)and photosynthetic water use efficiency,but it reduced stomatal conductance and transpiration.The stimulation of photosynthesis by elevated CO_(2)was greatest at 50%light and smallest at 100%.Photosynthesis,maximum carboxylation rate(Vcmax)and light-saturated rate of electron transport(Jmax)all decreased with decreasing light.Elevated CO_(2)significantly reduced Vcmax across all light treatments and both species in mid-growing season.However,the effect of elevated CO_(2)became insignificant at 30%light later in the growing season,with the response being greater in black spruce than in white spruce.Elevated CO_(2)also reduced Jmax in white spruce in both measurements while the effect became insignificant at 30%light later in the growing season.However,the effect on black spruce varied with time.Elevated CO_(2)reduced Jmax in black spruce in mid-growing season in all light treatments and the effect became insignificant at 30%light later in the growing season,while it increased Jmax later in the season at 100%and 50%light.These results suggest that both species benefited from elevated CO_(2),and that the responses varied with light supply,such that the response was primarily physiological at 100%and 50%light,while it was primarily morphological at 30%light.
