目的:观察《武威汉代医简》之五劳七损方治疗产后痹的临床疗效。方法:将85例产后痹患者采用随机数字表法随机分为治疗组44例和对照组组40例,治疗组给予五劳七损方,1 d 1剂,分2次口服;对照组给予口服益肾蠲痹丸,8 g/次,3次/d。两组均以1...目的:观察《武威汉代医简》之五劳七损方治疗产后痹的临床疗效。方法:将85例产后痹患者采用随机数字表法随机分为治疗组44例和对照组组40例,治疗组给予五劳七损方,1 d 1剂,分2次口服;对照组给予口服益肾蠲痹丸,8 g/次,3次/d。两组均以1个月为1个疗程,连续治疗3个疗程后判定疗效。结果:治疗组临床控制17例,显效13例,有效12例,无效2例,有效率占95.46%;对照组临床控制9例,显效10例,有效12例,无效9例,有效率占77.50%。两组对比,差别有统计学意义(P<0.05)。结论:《武威汉代医简》之五劳七损方治疗产后痹,疗效确切。展开更多
Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustio...Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustion method and Rubrospinal Tract(RST)transection were combined to establish arat model of SCI with pathopattern of QDBS in TCM.Macroscopic representation,including the body weight,food intake and tongue scores,was recorded.Behavioristics was scored with spontaneous vertical exploration test.Hemorrheology was analyzed using hemorheological analyzer and morphology of spinal cord was observed.The feasibility of the model was confirmed on the basis of the reversion caused by administration of Bu Yang Huan Wu Decoction(BYHWD).Results The findings in the QDBS group were consistent with the symptoms of QDBS syndrome,including reduced body weight,food intake,and forelimb utilization rate and increased tongue scores.However,the corresponding findings in the BYHWD group were superior to those in the QDBS group after35d(P<0.05).In comparison with the control group,the QDBS group showed higher whole blood viscosity and hematocrit values(P<0.05)and a lower red blood cell(RBC)deformability index(P<0.05),while the corresponding findings in the BYHWD group were close to those in the control group.An eminence or induration appeared in the injured spinal cord,which was suspected to be vacuoles or a scar.Hematoxylin-eosin staining showed that the R/T ratio in the BYHWD group was higher than that in the QDBS group(P<0.05).Conclusion Swimming exhaustion method combined with RST transection operation can successfully establish a rat model of SCI with pathopattern of QDBS.展开更多
OBJECTIVE:To investigate the gastroprotective effects of Acanthopanax senticosus leaves(ASLs)extrusion on acute gastric mucosal lesion in rats induced by compound 48/80(C48/80).METHODS:Rats were divided into six group...OBJECTIVE:To investigate the gastroprotective effects of Acanthopanax senticosus leaves(ASLs)extrusion on acute gastric mucosal lesion in rats induced by compound 48/80(C48/80).METHODS:Rats were divided into six groups:normal;C48/80-induced gastric lesion control;gastric lesion positive control(famotidine 4 mg/kg);gastric lesion administered with two levels of extruded ASLs(ASLE,40 and 200 mg/kg);and gastric lesion treated with ASLs(ASL 200 mg/kg).Mucus secretion/damage was determined by immunohistological staining.Immunofluorescence and western blotting were performed to determine gastric mucosal Bax and Bcl-2 expression.Gastric mucosal oxidative-stress-related enzymes and malondialdehyde were determined.RESULTS:C48/80-induced mucus depletion and inflammation in the gastric mucosa were significantly attenuated by ASLs.The increased serum serotonin and histamine concentrations in C48/80-treated rats were also attenuated by ASLs.Gastric mucosal Bax protein expression was increased and Bcl-2 expression was decreased after C48/80 treatment,and ASLs ameliorated Bax and Bcl-2 expression.The extrusion process significantly augmented the effects of ASLs in a dosedependent manner.ASLEs at 200 mg/kg normalized mucus damage/secretion,C48/80-induced increases of mucosal myeloperoxidase activity(index of inflammation),xanthine oxidase,and malondialdehyde content(index of lipid peroxidation).The effects of ASLs on Bax and Bcl-2expression were also enhanced by extrusion.Furthermore,these effects of ASLEs at 200 mg/kg were similar to those of famotidine,a histamine H2-receptor antagonist commonly used to treat gastric ulcers.CONCLUSION:ASLEs prevented acute gastric mucosal lesion progression induced by C48/80,possibly by inducing mucus production,and reduced inflammation and oxidative stress in gastric mucosa through an anti-apoptotic mechanism.展开更多
文摘目的:观察《武威汉代医简》之五劳七损方治疗产后痹的临床疗效。方法:将85例产后痹患者采用随机数字表法随机分为治疗组44例和对照组组40例,治疗组给予五劳七损方,1 d 1剂,分2次口服;对照组给予口服益肾蠲痹丸,8 g/次,3次/d。两组均以1个月为1个疗程,连续治疗3个疗程后判定疗效。结果:治疗组临床控制17例,显效13例,有效12例,无效2例,有效率占95.46%;对照组临床控制9例,显效10例,有效12例,无效9例,有效率占77.50%。两组对比,差别有统计学意义(P<0.05)。结论:《武威汉代医简》之五劳七损方治疗产后痹,疗效确切。
基金funding support from the National Natural Science Foundation of China (No. 81302899, 81373551 and 81603512)the Key Science and Research Program of Hunan Department of Science and Technology (No. 2012TF-1005)
文摘Objective To establish a rat model of Spinal Cord Injury(SCI)with the pathopattern of Qi-deficiency and Blood-stasis(QDBS)in Traditional Chinese Medicine(TCM),and then assess its feasibility.Methods Swimming exhaustion method and Rubrospinal Tract(RST)transection were combined to establish arat model of SCI with pathopattern of QDBS in TCM.Macroscopic representation,including the body weight,food intake and tongue scores,was recorded.Behavioristics was scored with spontaneous vertical exploration test.Hemorrheology was analyzed using hemorheological analyzer and morphology of spinal cord was observed.The feasibility of the model was confirmed on the basis of the reversion caused by administration of Bu Yang Huan Wu Decoction(BYHWD).Results The findings in the QDBS group were consistent with the symptoms of QDBS syndrome,including reduced body weight,food intake,and forelimb utilization rate and increased tongue scores.However,the corresponding findings in the BYHWD group were superior to those in the QDBS group after35d(P<0.05).In comparison with the control group,the QDBS group showed higher whole blood viscosity and hematocrit values(P<0.05)and a lower red blood cell(RBC)deformability index(P<0.05),while the corresponding findings in the BYHWD group were close to those in the control group.An eminence or induration appeared in the injured spinal cord,which was suspected to be vacuoles or a scar.Hematoxylin-eosin staining showed that the R/T ratio in the BYHWD group was higher than that in the QDBS group(P<0.05).Conclusion Swimming exhaustion method combined with RST transection operation can successfully establish a rat model of SCI with pathopattern of QDBS.
基金Supported by Cooperative Research Program for Agriculture Science and Technology Development and Rural Development Administration,Republic of Korea(Investigation of Functional Nutrients from Agricultural Food and Development of Integrated Information,No.PJ011644)
文摘OBJECTIVE:To investigate the gastroprotective effects of Acanthopanax senticosus leaves(ASLs)extrusion on acute gastric mucosal lesion in rats induced by compound 48/80(C48/80).METHODS:Rats were divided into six groups:normal;C48/80-induced gastric lesion control;gastric lesion positive control(famotidine 4 mg/kg);gastric lesion administered with two levels of extruded ASLs(ASLE,40 and 200 mg/kg);and gastric lesion treated with ASLs(ASL 200 mg/kg).Mucus secretion/damage was determined by immunohistological staining.Immunofluorescence and western blotting were performed to determine gastric mucosal Bax and Bcl-2 expression.Gastric mucosal oxidative-stress-related enzymes and malondialdehyde were determined.RESULTS:C48/80-induced mucus depletion and inflammation in the gastric mucosa were significantly attenuated by ASLs.The increased serum serotonin and histamine concentrations in C48/80-treated rats were also attenuated by ASLs.Gastric mucosal Bax protein expression was increased and Bcl-2 expression was decreased after C48/80 treatment,and ASLs ameliorated Bax and Bcl-2 expression.The extrusion process significantly augmented the effects of ASLs in a dosedependent manner.ASLEs at 200 mg/kg normalized mucus damage/secretion,C48/80-induced increases of mucosal myeloperoxidase activity(index of inflammation),xanthine oxidase,and malondialdehyde content(index of lipid peroxidation).The effects of ASLs on Bax and Bcl-2expression were also enhanced by extrusion.Furthermore,these effects of ASLEs at 200 mg/kg were similar to those of famotidine,a histamine H2-receptor antagonist commonly used to treat gastric ulcers.CONCLUSION:ASLEs prevented acute gastric mucosal lesion progression induced by C48/80,possibly by inducing mucus production,and reduced inflammation and oxidative stress in gastric mucosa through an anti-apoptotic mechanism.
