井穴点刺出血对局灶性脑缺血大鼠脑梗塞体积影响的基础研究

目的 :观察井穴点刺出血对局灶性脑缺血大鼠脑梗塞体积的影响。方法 :将 4 2只大鼠随机分成4组 (正常对照组、模型组、井穴治疗组、假手术组 ) ,并用线栓法制成局灶性脑缺血永久性栓塞模型 ,通过TTC染色测定脑梗死体积。结论 :急性脑梗塞早期采用井穴点刺出血可明显降低脑梗死体积 ,其疗效肯定 (P <0 .0 1) ,而早期采用体针治疗作用不明显。

电针结合井穴点刺放血治疗腕管综合征临床疗效观察

目的:观察电针结合井穴点刺放血治疗腕管综合征(CTS)的临床疗效。方法:选取本院2013年6月至2016年9月收治的96例轻中度腕管综合征患者进行研究,将所选患者按照随机数字表法分别分配到观察组与对照组,每组各48例,观察组患者给予电针结合井穴点刺放血治疗,对照组患者给予电针治疗。回顾性分析所选患者的临床资料,对比分析两组患者治疗后的临床疗效及复发率。结果:治疗后,观察组总有效率为95.83%,对照组总有效率为77.08%,两组进行对比,观察组明显高于对照组(P<0.05)。治疗6个月后,观察组与对照组患者进行复发率的比较,观察组明显低于对照组,差异有统计学意义(P<0.05);治疗12个月后,观察组与对照组患者进行复发率的比较,观察组明显低于对照组,差异有统计学意义(P<0.05)。结论:电针结合井穴点刺放血治疗CTS患者,既能调节经络功能,促进组织代谢,消除炎症,改善感觉障碍,操作简单,恢复神经感觉功能疗效好,值得临床推广。

针刺对大鼠脑缺血超早期脑细胞内外游离钙离子浓度的影响

目的:观察手十二井穴点刺放血法对大鼠脑缺血超早期脑细胞内游离C a2+浓度(〔C a2+〕i)和细胞外游离C a2+浓度(〔C a2+〕o)的干预作用,探讨其在中风急救中的作用机制。方法:30只W istar大鼠随机分为假手术组、模型组和治疗组。应用针型C a2+选择电极观测大鼠脑缺血即刻至20 m in期间每分钟脑缺血区皮质细胞〔C a2+〕o的变化。应用Fura 2/AM荧光探针技术观测脑缺血后20 m in脑缺血区皮质神经元突触体内胞浆〔C a2+〕i。结果:与假手术组比较,模型组大鼠造成脑缺血后,缺血区皮质细胞〔C a2+〕o从第5 m in开始出现明显降低(P<0.05),到20 m in为止其下降幅度与时间呈正相关(P<0.01),而缺血区皮质神经元突触体内胞浆〔C a2+〕i显著增高(P<0.01)。与模型组比较,治疗组大鼠脑缺血后缺血区皮质细胞〔C a2+〕o从第8 m in开始其下降幅度明显减小(P均<0.05),到第18 m in此趋势更加显著(P均<0.01),第20 m in缺血区皮质神经元突触体内胞浆〔C a2+〕i显著降低(P<0.01)。结论:脑缺血超早期应用手十二井穴点刺放血法进行干预可快速起效,起到调节缺血区脑细胞内外游离C a2+浓度,有效抑制神经元内钙超载,保护脑细胞功能的作用。

Muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting in the treatment of shoulder-hand syndrome after stroke

Objective To observe the effect of muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting in improving nail fold microcirculation in the patients with shoulder-hand syndrome(SHS)after stroke,and the effects on hemorrheology,calcitonin gene-related peptide(CGRP)and serum substance P(SP).Methods A total of 72 patients were randomized into an observation group and a control group by the random number table method,with 36 cases in each group.The control group was treated with physical rehabilitation training,and the observation group was treated with additional muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting treatment.The treatment course lasted for 4 weeks.After treatment,the clinical efficacy of the two groups was compared.The changes in shoulder-hand syndrome scale(SHSS),simplified Fugl-Meyer assessment-upper extremity(FMA-UE),visual analog scale(VAS),activities of daily living(ADL),traditional Chinese medicine(TCM)syndrome score,nail fold microcirculation hemorheology indictors[whole blood viscosity(high-shear,low-shear),hematocrit,erythrocyte sedimentation rate(ESR)],CGRP and SP levels were observed.Results The total effective rate in the observation group was 86.1%,higher than 63.9%in the control group(P<0.05).The overall curative effect in the observation group was better than that in the control group(P<0.05).After treatment,the scores of pain sensation,edema,external turn and rotation of the arm in SHSS,and the total score were significantly decreased in both groups(all P<0.05),and each score in the observation group was lower than that in the control group(all P<0.05).After treatment,the scores of VAS and TCM syndrome in both groups decreased significantly(all P<0.05),and the scores of FMA-UE and ADL increased significantly(all P<0.05).The scores of VAS and TCM syndrome in the observation group were lower than those in the control group(both P<0.05),and the scores of FMA-UE and ADL were higher than those in the control group(both P<0.05).After treatment,the whole blood viscosity(high-shear and low-shear)and hematocrit in both groups decreased obviously(all P<0.05),and ESR increased obviously(both P<0.05),and the whole blood viscosity(high-shear and low-shear)and hematocrit in the observation group were lower than those in the control group(all P<0.05),and ESR was higher than that in the control group(P<0.05).After treatment,the peritubular state,loop shape,blood flow and total score of nail fold microcirculation in both groups decreased significantly(all P<0.05),and each score in the observation group was lower than that in the control group(all P<0.05).After treatment,SP in both groups decreased obviously(both P<0.05),CGRP increased obviously(both P<0.05),and SP in the observation group was lower than that in the control group(P<0.05),CGRP was higher than that in the control group(P<0.05).Conclusion Compared with conventional physical rehabilitation training,muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting treatment can significantly reduce the clinical symptoms of SHS,promote the recovery of physical functions,improve the nail fold microcirculation and hemorrheology indictors,and regulate the serum cytokine levels such as CGRP and SP.