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HPLC法测定栽培亚大黄中3种成分的含量 被引量:5
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作者 耿家玲 沈勇 康绍建 《中国药师》 CAS 2011年第5期666-668,共3页
目的:建立HPLC法分离和测定亚大黄药材中虎杖苷、白藜芦醇、甲基虎杖苷的含量。方法:色谱柱:C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-甲醇-0.1%磷酸(16:8:76)梯度洗脱,检测波长:306 nm。结果:虎杖苷在0.009 1~0.454 0μg;白藜... 目的:建立HPLC法分离和测定亚大黄药材中虎杖苷、白藜芦醇、甲基虎杖苷的含量。方法:色谱柱:C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-甲醇-0.1%磷酸(16:8:76)梯度洗脱,检测波长:306 nm。结果:虎杖苷在0.009 1~0.454 0μg;白藜芦醇在0.000 6~0.030 4μg;甲基虎杖苷在0.047 4~2.370 0μg范围内与峰面积呈良好的线性关系,平均回收率分别为98.82%(RSD=1.2%),98.33%(RSD=2.1%),99.40%(RSD=1.5%)。结论:本方法可作为亚大黄药材质量控制手段之一。 展开更多
关键词 亚大黄 虎杖苷 白藜芦醇 甲基虎杖苷 HPLC法
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四川产亚大黄的鉴别及其大黄素、大黄酚的测定 被引量:4
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作者 张力 马静 +4 位作者 欧洋 张朝阳 李卒 王玲玲 王曙 《华西药学杂志》 CAS CSCD 2015年第3期322-324,共3页
目的鉴别四川产亚大黄,并测定其大黄素、大黄酚的含量。方法采用显微鉴别、TLC法对药材进行鉴别,用HPLC法测定大黄素、大黄酚的含量。结果 TLC法鉴别亚大黄的专属性强;建立了HPLC测定疏枝大黄中大黄素、大黄酚含量的方法。结论所用方法... 目的鉴别四川产亚大黄,并测定其大黄素、大黄酚的含量。方法采用显微鉴别、TLC法对药材进行鉴别,用HPLC法测定大黄素、大黄酚的含量。结果 TLC法鉴别亚大黄的专属性强;建立了HPLC测定疏枝大黄中大黄素、大黄酚含量的方法。结论所用方法操作简便、结果准确、重复性好,可用于四川产亚大黄的质量控制。 展开更多
关键词 亚大黄 质量控制 显微鉴别 薄层色谱法 高效液相色谱法
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基于UPLC-Q-TOF-MS/MS快速分析亚大黄中的抗补体活性成分 被引量:7
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作者 詹艳芝 吴蓓 +6 位作者 黄文平 徐旭 张晶 陈思思 冯育林 杨世林 温泉 《中国实验方剂学杂志》 CAS CSCD 北大核心 2018年第10期60-66,共7页
目的:通过UPLC-Q-TOF-MS/MS技术快速分析亚大黄中的抗补体活性成分群,为阐明亚大黄的抗补体活性药效物质基础提供参考。方法:对亚大黄95%醇提物进行萃取得到石油醚、乙酸乙酯、正丁醇3个部位,并测定3个部位经典途径的抗补体溶血活性(... 目的:通过UPLC-Q-TOF-MS/MS技术快速分析亚大黄中的抗补体活性成分群,为阐明亚大黄的抗补体活性药效物质基础提供参考。方法:对亚大黄95%醇提物进行萃取得到石油醚、乙酸乙酯、正丁醇3个部位,并测定3个部位经典途径的抗补体溶血活性(CH50);采用UPLC-Q-TOF-MS/MS对3个部位分别进行化学成分的表征,使用质谱分析软件中的目标化合物筛查法,通过保留时间、精确相对分子质量和二级质谱裂解碎片鉴定化学成分。结果:亚大黄正丁醇部位抗补体活性为0.032 g·L^-1,强于阳性药肝素钠;在优化的LC-MS条件下,结合Scifinder数据库、对照品和相关文献,鉴定了石油醚部位主要成分9个,主要是脂肪酸及甾醇类化合物;鉴定了乙酸乙酯部位主要成分13个,主要是游离蒽醌类和二苯乙烯类;鉴定了正丁醇部位主要成分25个,主要是蒽醌苷类和二苯乙烯苷类。结论:基于UPLC-Q-TOF-MS/MS技术,快捷、准确、较全面地分析鉴定亚大黄的正丁醇抗补体活性部位的化学成分主要为蒽醌苷和二苯乙烯苷类,为一步分离及开发亚大黄中苷类抗补体活性成分提供科学依据。 展开更多
关键词 亚大黄 化学成分 抗补体 超高液相色谱四级杆飞行时间质谱 清热解毒
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HPLC法测定藏药亚大黄中黄酮类成分的研究 被引量:1
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作者 张宁 常春 傅强 《高原医学杂志》 CAS 2013年第4期55-56,共2页
目的:建立藏药亚大黄的质量控制方法。方法:采用甲醇-0.4%磷酸溶液(4060)为流动相,检测波长257nm,柱温:35℃,HPLC法测定亚大黄中芦丁的含量。结果:芦丁的标准工作曲线为:Y=9 554 611.08X+43 219.32,R=0.999 2,芦丁在0.11μg^2.21μg... 目的:建立藏药亚大黄的质量控制方法。方法:采用甲醇-0.4%磷酸溶液(4060)为流动相,检测波长257nm,柱温:35℃,HPLC法测定亚大黄中芦丁的含量。结果:芦丁的标准工作曲线为:Y=9 554 611.08X+43 219.32,R=0.999 2,芦丁在0.11μg^2.21μg的范围内呈良好的线性关系,平均回收率为98.6%,RSD为2.1%(n=5)。结论:建立HPLC法研究亚大黄药材及其制剂的质量控制,更准确的控制药材的质量。 展开更多
关键词 亚大黄 HPLC 芦丁
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青藏高原大黄属植物在藏药中的应用 被引量:8
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作者 罗达尚 左振常 +1 位作者 夏光成 刘丽 《中草药》 CAS 1985年第8期31-33,共3页
本文主要介绍大黄属植物在藏药中的应用。青藏高原大黄属植物有28种,其中藏药所用21种。藏医根据大黄的质量,疗效的优次将它分为上、中、下三品,其临床范围相同,但在临床剂量和选用何种,则根据患者的病情而定。
关键词 亚大黄 小大 藏药 晶珠本草
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Acid Sulfate Soils in Australia: Characteristics,Problemsand Management 被引量:3
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作者 C. LIN(School of Resource Science and Management, Southern Cross Universitg, Lismore, N.S. W. 248O (Australia)) 《Pedosphere》 SCIE CAS CSCD 1999年第4期289-298,共10页
Acid soils(ASS) are widely distributed in Australia. This has only been recognized recentlywhen intensive research on ASS has been done in this country. This paper reviews aspects concerning a)the distribution and aci... Acid soils(ASS) are widely distributed in Australia. This has only been recognized recentlywhen intensive research on ASS has been done in this country. This paper reviews aspects concerning a)the distribution and acid potential, b) controls on acidic status, and c) problems and management of ASSin Australia It is believed that the Australian experience may be useful for other countries where potentialproblems from ASS exist but insrfficient attention was paid to them. 展开更多
关键词 acid sulfate soils AUSTRALIA estuarine floodplain MANAGEMENT PYRITE
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Iron Monosulfide Distribution in Three Coastal Floodplain Acid Sulfate Soils, Eastern Australia 被引量:3
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作者 R. T. BUSH, L. A. SULLIVAN and C. LIN School of Resource Science and Management, Southern Cross University, Lismore, NSW 2480 (Australia) 《Pedosphere》 SCIE CAS CSCD 2000年第3期237-245,共9页
The distribution of iron monosulfide (quantified as acid volatile sulfur: SAV) was compared with geo- chemical properties that are known to affect its formation and accumulation in three coastal Holocene acid sulfate ... The distribution of iron monosulfide (quantified as acid volatile sulfur: SAV) was compared with geo- chemical properties that are known to affect its formation and accumulation in three coastal Holocene acid sulfate soils (ASS) at Tuckean Swamp, McLeods Creek and Bungawalbyn Swamp respectively. These properties included PH, reactive iron (FeR), pore-water sulfate (SO:42-) and organic carbon (OC). Iron monosulfide was concentrated at the oxic/anoxic boundary. The Tuckean Swamp and McLeods Creek sites are Holocene sediments, whereas the Bungawalbyn Swamp is a Holocene peat. The concentration of SAV averaged 0.2 g kg-l in a 0.5 m thick soil layer at the Tuckean Swamp, but was an order of magnitude lower in the oxic/anoxic transition layers at McLeods Creek and Bungawalbyn Swamp. The SAV mineral greigite (Fe3S4) was identified in the Tuckean Swamp by X-ray diffraction and scanning electron microscopy with quantitative energy dispersive X-ray analysis (SEM-EDX). Very small concentrations of greigite were also observed in the McLeods Creek, based on crystal morphology and elemental composition. The concentration of SAV was a small fraction of the total reduced sulfur, representing at most 3% of the Pyrite sulfur. However, the presence of this highly reactive sulfide mineral, distributed within pores where oxygen diffusion is most rapid, has important implications to the potential rate of acid production from these sediments. 展开更多
关键词 acid sulfate soils monosulfide PYRITE OXIDATION sulfid
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藏边大黄的质量标准研究 被引量:4
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作者 王薛 王曙 叶本贵 《华西药学杂志》 CAS CSCD 2019年第4期370-374,共5页
目的建立藏边大黄的质量标准。方法采用原植物鉴别、性状鉴别、显微鉴别、理化鉴别四大经典鉴别法鉴别藏边大黄;按2015年版《中国药典》附录中的方法测定藏边大黄的干燥失重、总灰分和浸出物;采用HPLC法测定药材中指标性成分藏黄苷的含... 目的建立藏边大黄的质量标准。方法采用原植物鉴别、性状鉴别、显微鉴别、理化鉴别四大经典鉴别法鉴别藏边大黄;按2015年版《中国药典》附录中的方法测定藏边大黄的干燥失重、总灰分和浸出物;采用HPLC法测定药材中指标性成分藏黄苷的含量。结果四大鉴别均具显著特征,可作为藏边大黄药材的鉴别方法;并初步拟定了藏边大黄中水分的含量不得过15.0%,总灰分的含量不得过10.0%,浸出物的含量不得少于25.0%,藏黄苷A的含量不得少于3.0%。结论所用方法结果准确,重复性、稳定性均较好,可为藏边大黄药材质量标准的制定提供实验依据。 展开更多
关键词 藏边大 亚大黄 质量标准 原植物鉴别 性状鉴别 显微鉴别 理化鉴别 高效液相色谱法 苷A 含量测定
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Onset of Xiashu loess deposition in southern China by 0.9 Ma and its implications for regional aridification 被引量:13
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作者 Xusheng LI Zhiyong HAN +6 位作者 Huayu LU Yingyong CHEN Yang LI Xiaokang YUAN Yuwen ZHOU Mengyao JIANG Cunjuan LV 《Science China Earth Sciences》 SCIE EI CAS CSCD 2018年第3期256-269,共14页
The Xiashu loess is a typical Quaternary eolian deposit in southem China and represents an important terrestrial paleoclimate archive in this low-latitude monsoon region. However, the chronological framework of Xiashu... The Xiashu loess is a typical Quaternary eolian deposit in southem China and represents an important terrestrial paleoclimate archive in this low-latitude monsoon region. However, the chronological framework of Xiashu loess deposition has yet to be established. Determining the timing of the onset of Xiashu loess deposition will allow researchers to better understand late Quaternary aridification across the Asian continent, the evolution of the East Asian monsoon and regional environmental changes in subtropical regions. Therefore, in this study, a systematic chronological study of the Xiashu loess is conducted to answer this question. For the first time, magnetostratigraphic classification reveals that the Matuyama/Bnmhes (M/B) reversal is present in the Xiashu loess at two sites in Jiangsu Province, the Qingshan profile at Yizheng and the Dagang core in Zhenjiang. Based on the results of magnetostratigraphy and optically stimulated luminescence (OSL) dating, the age of the lower boundary of the Xiashu loess is estimated to be approximately 0.9 Ma. Consequently, this Xiashu loess deposit is the oldest reported to date and is comparable in age to the red soil deposit in Xuancheng, Anhui Province. The onset of Xiashu loess deposition by 0.9 Ma represents the further expansion of arid range in Asia in the late Quaternary in response to significant aridification and winter monsoon strengthening in this subtropical region. We suggest that these climate changes were primarily driven by global cooling and an increase in high-latitude ice volume in the Northern Hemisphere and that the initiation of Xiashu loess accumulation was a regional response of southern China to the 0.9 Ma global cooling event. 展开更多
关键词 Xiashu loess CHRONOLOGY Magnetic stratigraphy ARIDIFICATION East Asian monsoon
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