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亚栖热菌产海藻糖合酶培养基的优化 被引量:2
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作者 陈晓云 张峻 +2 位作者 陈颖 齐欣 魏雪生 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第1期27-32,共6页
对亚栖热菌 CBS-01菌株发酵生产海藻糖合酶的培养基进行了优化.首先通过碳源、氮源实验,选出了适宜的碳、氮源,随后采用 Plackett-Burman 设计法对培养基中的组分进行了筛选,找出影响产酶的主要因素为蛋白胨和酵母膏,最后利用中心组合... 对亚栖热菌 CBS-01菌株发酵生产海藻糖合酶的培养基进行了优化.首先通过碳源、氮源实验,选出了适宜的碳、氮源,随后采用 Plackett-Burman 设计法对培养基中的组分进行了筛选,找出影响产酶的主要因素为蛋白胨和酵母膏,最后利用中心组合设计及响应面分析法求出了主要影响因素的最佳浓度,得到优化的发酵培养基配方(g/L):可溶性淀粉5,蛋白胨8.6,酵母膏1.725,NaNO_3 0.7,氨三乙酸0.5,Na_2HPO_4 0.1,MgSO_4·7H_2O 0.2,CaCl_2 0.1.10 L 罐发酵实验表明,CBS-01菌株海藻糖合酶的合成属生长关联型,利用优化的发酵培养基,CBS-01菌株的产酶水平由0.2 U/mL 提高到0.41 U/m L. 展开更多
关键词 亚栖热菌 海藻糖合酶 Plackett—Burman设计 响应面分析法
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红色亚栖热菌海藻糖合酶在大肠杆菌中的表达及其酶学性质分析 被引量:1
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作者 王文文 张峻 +5 位作者 王宇凡 朱玥明 张娟 刘艳超 邢来君 李明春 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第3期90-97,共8页
利用前期克隆得到的红色亚栖热菌CBS-01(Meiothermus ruber CBS-01)的海藻糖合酶基因(TreS),构建天然蛋白异源宿主表达载体并在大肠杆菌中获得较高的表达量.经纯化后测定该酶在pH 6.5,反应温度为50℃时活性最高.酶学性质的研究表明,Tre... 利用前期克隆得到的红色亚栖热菌CBS-01(Meiothermus ruber CBS-01)的海藻糖合酶基因(TreS),构建天然蛋白异源宿主表达载体并在大肠杆菌中获得较高的表达量.经纯化后测定该酶在pH 6.5,反应温度为50℃时活性最高.酶学性质的研究表明,TreS的最适底物为麦芽糖,其对麦芽糖的转化效率约是海藻糖的2.5倍;该酶在30~60℃、pH 3.5~9.0范围内都保留较高的酶活;2 mmol/L的一价阳离子Na^+、K^+对TreS有激活作用;低温有利于反应向海藻糖方向进行,并能减少葡萄糖副产物的生成;20℃时,几乎没有葡萄糖生成,麦芽糖至海藻糖的转化率可达65%.为工业化大规模生产海藻糖奠定了理论基础. 展开更多
关键词 红色亚栖热菌 海藻糖合酶(TreS) 表达 酶学性质
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亚栖热菌透性化细胞的耦合固定化研究 被引量:1
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作者 张峻 陈颖 +2 位作者 陈晓云 齐欣 魏雪生 《中国生物工程杂志》 CAS CSCD 北大核心 2007年第11期32-36,共5页
将海藻酸盐凝胶包埋法与交联法和聚电解质静电自组装覆膜法相耦合,对含有海藻糖合酶活性的亚栖热菌的透性化细胞进行了固定化研究。结果表明,利用重氮树脂和聚苯乙烯磺酸钠对海藻酸凝胶微球交替覆膜,可以显著提高凝胶微球在磷酸盐缓冲... 将海藻酸盐凝胶包埋法与交联法和聚电解质静电自组装覆膜法相耦合,对含有海藻糖合酶活性的亚栖热菌的透性化细胞进行了固定化研究。结果表明,利用重氮树脂和聚苯乙烯磺酸钠对海藻酸凝胶微球交替覆膜,可以显著提高凝胶微球在磷酸盐缓冲液中的稳定性,以碳二亚胺对固定化细胞进行交联处理则可以提高固定化细胞中海藻糖合酶的热稳定性。透性化细胞经包埋-交联-覆膜耦合固定化后,酶活回收率为32%,最适酶反应pH值由6.5左右升至7.0左右,最适反应温度未变,仍为60℃。所得固定化细胞间歇反应时,催化麦芽糖转化为海藻糖的转化率可达60%,重复使用4次(每次50℃、反应24h),酶活损失小于20%,转化率可保持在50%以上。 展开更多
关键词 亚栖热菌 海藻糖合酶 耦合固定化
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云南热泉亚栖热菌质粒与其部分表型关系的初步研究
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作者 唐嘉 陈朝银 +1 位作者 林先志 张涛 《云南化工》 CAS 2007年第2期71-74,共4页
从云南洱源、腾冲热泉分离到108株专性嗜热非芽孢菌,经形态、革兰氏染色及生理生化实验初步鉴定为亚栖热菌。通过碱裂解及试剂盒抽提质粒,琼脂糖凝胶电泳检测,13株菌含有质粒。用AO、SDS对RH17-H进行化学消除后对比消除前后色素和耐高... 从云南洱源、腾冲热泉分离到108株专性嗜热非芽孢菌,经形态、革兰氏染色及生理生化实验初步鉴定为亚栖热菌。通过碱裂解及试剂盒抽提质粒,琼脂糖凝胶电泳检测,13株菌含有质粒。用AO、SDS对RH17-H进行化学消除后对比消除前后色素和耐高温性变化,显示与质粒无决定性关系。 展开更多
关键词 亚栖热菌 质粒 消除
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红色亚栖热菌耐热木聚糖酶的克隆表达及生物信息学分析 被引量:1
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作者 俞洁琼 王洪成 +2 位作者 何正文 乐易林 邵蔚蓝 《江苏农业学报》 CSCD 北大核心 2015年第6期1242-1249,共8页
从自主分离的Meiothermus.rubber菌株的基因组中克隆到木聚糖酶基因,并进行了超量表达、重组酶的纯化和酶学性质研究。结果表明,重组木聚糖酶Mru是热稳定性酶,最适反应温度为65℃,能够有效降解木聚糖产生木糖和木二糖。生物信息学分析发... 从自主分离的Meiothermus.rubber菌株的基因组中克隆到木聚糖酶基因,并进行了超量表达、重组酶的纯化和酶学性质研究。结果表明,重组木聚糖酶Mru是热稳定性酶,最适反应温度为65℃,能够有效降解木聚糖产生木糖和木二糖。生物信息学分析发现,木聚糖酶Mru与数据库中来自亚栖热菌的木聚糖酶的同源性为60%~100%。在木聚糖酶Mru的结构模型中,GH10木聚糖酶的保守位点大多位于β折叠,α螺旋保守性很低。本研究揭示,木聚糖酶Mru代表一类新型热稳定性GH10木聚糖酶。 展开更多
关键词 红色亚栖热菌 木聚糖酶 生物信息学分析 高温酶
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传唐代书家释亚栖《论书》考辨
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作者 熊俊屹 《中国书画》 2021年第11期9-11,共3页
释亚栖,洛阳人,生卒年不详,是晚唐著名的僧人书法家。他擅长草书,与同为晚唐僧人书家的?光齐名,惜无作品传世。后代对他的评价褒贬不一,例如《宣和书谱》本传谓其"得张颠笔意",因而"对殿庭草书,两赐紫袍"^([1]),受... 释亚栖,洛阳人,生卒年不详,是晚唐著名的僧人书法家。他擅长草书,与同为晚唐僧人书家的?光齐名,惜无作品传世。后代对他的评价褒贬不一,例如《宣和书谱》本传谓其"得张颠笔意",因而"对殿庭草书,两赐紫袍"^([1]),受到了不小的恩宠。然而同书在李霄远条下又谈道:"至于字法之坏,则实由亚栖……俗子之所深喜,而未免夫知书者之所病也。"^([2])可谓贬斥至极。对此,北宋书家周越也有类似的看法,桑世昌《兰亭考》引用了《周子发帖》(即周越的法书)对亚栖的评价。 展开更多
关键词 洛阳人 兰亭考 张颠 周越 生卒年 草书 亚栖
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红色亚栖热菌TPS/TPP海藻糖合成途径中相关基因的克隆、表达及功能鉴定
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作者 朱玥明 唐亦辰 +4 位作者 徐恒毅 张娟 魏东盛 邢来君 李明春 《生物工程学报》 CAS CSCD 北大核心 2009年第3期399-405,共7页
通过构建红色亚栖热菌(Meiothermus ruberCBS-01)的基因组DNA文库,克隆得到该嗜热菌海藻糖合成途径中的磷酸海藻糖合成酶(TPS)和磷酸海藻糖磷酸酯酶(TPP)基因。以pET21a为表达载体,将磷酸海藻糖合成酶和磷酸海藻糖磷酸酯酶在大肠杆菌中... 通过构建红色亚栖热菌(Meiothermus ruberCBS-01)的基因组DNA文库,克隆得到该嗜热菌海藻糖合成途径中的磷酸海藻糖合成酶(TPS)和磷酸海藻糖磷酸酯酶(TPP)基因。以pET21a为表达载体,将磷酸海藻糖合成酶和磷酸海藻糖磷酸酯酶在大肠杆菌中进行表达并纯化,利用薄层层析的方法验证了这两个酶的活性。同时,本研究检测了红色亚栖热菌在各种环境压力下细胞内含物成分的变化情况,发现在高渗环境压力的诱导下,该菌会在胞内积累大量的6-磷酸海藻糖,而并非海藻糖,这为进一步研究TPS/TPP和TreS途径在细胞体内的作用奠定了基础。 展开更多
关键词 红色亚栖热菌 海藻糖 海藻糖合成途径 磷酸海藻糖合成酶(TPS) 磷酸海藻糖磷酸酯酶(TPP)
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晚唐草书僧亚栖书法研究三题
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作者 蒋全顺 《书法》 2022年第3期188-191,共4页
笔者通过对晚唐草书僧亚栖相关书法资料的收集、整理,试从亚栖的书法创作类型及相关书法活动、亚栖的书学思想、亚栖书法的接受三个方面加以研究,以期全面、立体的展现晚唐草书僧亚栖的书法世界。
关键词 书法 亚栖 考述 贬抑
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透性化细胞海藻糖合酶的制备及其性质研究 被引量:14
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作者 张峻 陈晓云 +2 位作者 齐欣 陈颖 魏雪生 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第5期93-96,共4页
研究了亚栖热菌CBS-01菌株细胞的渗透处理工艺,并对所得透性化细胞海藻糖合酶的性质进行了考察.结果表明:以2%的甲苯为渗透剂,对10%浓度的菌悬液,50℃渗透处理30~60 min即得透性化细胞海藻糖合酶.该透性化细胞酶的最适反应pH为6.2~7.2... 研究了亚栖热菌CBS-01菌株细胞的渗透处理工艺,并对所得透性化细胞海藻糖合酶的性质进行了考察.结果表明:以2%的甲苯为渗透剂,对10%浓度的菌悬液,50℃渗透处理30~60 min即得透性化细胞海藻糖合酶.该透性化细胞酶的最适反应pH为6.2~7.2,最适反应温度为50~65℃;50℃时,催化麦芽糖生成海藻糖的转化率为70%. 展开更多
关键词 亚栖热菌 透性化细胞 海藻糖合酶 性质
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海藻酸锶凝胶固定化含海藻糖合酶细胞的研究 被引量:4
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作者 张峻 陈晓云 +2 位作者 陈颖 齐欣 魏雪生 《食品科学》 EI CAS CSCD 北大核心 2008年第3期332-335,共4页
含有海藻糖合酶的亚栖热菌CBS-01菌株细胞经过透性化处理后,以海藻酸锶凝胶对透性化细胞进行了固定化,当加酶量为12U/g海藻酸钠时,所得固定化细胞的载酶量可达6U/g海藻酸钠。细胞固定化后,最适酶反应pH值由6.5升至7.0,最适酶反应温度未... 含有海藻糖合酶的亚栖热菌CBS-01菌株细胞经过透性化处理后,以海藻酸锶凝胶对透性化细胞进行了固定化,当加酶量为12U/g海藻酸钠时,所得固定化细胞的载酶量可达6U/g海藻酸钠。细胞固定化后,最适酶反应pH值由6.5升至7.0,最适酶反应温度未变,为60℃,但热稳定性有所提高,70℃保温60min时,固定化细胞的残存酶活为60%,而未固定化细胞的残存酶活约30%。间歇反应时,固定化细胞催化麦芽糖转化为海藻糖的转化率可达60%,重复使用10次,仍可保持约70%的酶活。 展开更多
关键词 亚栖热菌 海藻糖合酶 固定化 海藻酸盐
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产结晶纤维素酶的嗜热菌鉴定及其酶学性质 被引量:4
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作者 吴窈画 邵蔚蓝 《南京师大学报(自然科学版)》 CAS CSCD 北大核心 2007年第2期84-88,共5页
从云南腾冲热海温泉采集的样品中,分离出一株产结晶纤维素酶的嗜热菌TC-1.该菌株呈杆状,革兰氏阴性,生理生化特征分析和16S rDNA序列同源性比对表明它属于亚栖热菌属Meiothermus.结晶纤维素、木聚糖和葡萄糖可以诱导该菌产生结晶纤维素... 从云南腾冲热海温泉采集的样品中,分离出一株产结晶纤维素酶的嗜热菌TC-1.该菌株呈杆状,革兰氏阴性,生理生化特征分析和16S rDNA序列同源性比对表明它属于亚栖热菌属Meiothermus.结晶纤维素、木聚糖和葡萄糖可以诱导该菌产生结晶纤维素酶,其中结晶纤维素诱导的酶活力最高.对其产生的结晶纤维素酶进行了初步纯化与酶学性质研究,该酶的最适反应温度为70℃,最适pH为6.0,在50℃~70℃和pH5.5~6.5之间酶活力相对稳定. 展开更多
关键词 亚栖热菌 16S RDNA 结晶纤维素酶 酶活 酶纯化和性质
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Biometrics and wing molt in White-winged Black Tern (Chlidonias leucopterus) in north-west Australia
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作者 Peter J. FULLAGAR Michael J. DAWKINS Clive D. T. MINTON 《Chinese Birds》 CSCD 2013年第4期306-313,共8页
We analyze morphometrics from a sample of 276 White-winged Black Tern(Chlidonias leucopterus) caught in north-west Australia on 4 March 2011. An estimated 40000 terns were present — the largest concentration of this ... We analyze morphometrics from a sample of 276 White-winged Black Tern(Chlidonias leucopterus) caught in north-west Australia on 4 March 2011. An estimated 40000 terns were present — the largest concentration of this species yet reported from Australia. When comparing juveniles with adults, only wing length and body mass differed significantly; however, juveniles were still easily recognized by plumage and wing molt as late as March. There was little evidence of any dramatic weight gain in adults before their northward migration. No morphological characteristic distinguishing sex was found. We confirmed that terns caught in this study did not appear to differ morphologically from those of the western palearctic populations. We describe wing molt based on data from 354 individuals captured and banded in Australia over 28 years. Wing molt proceeds in much the same way as found in other small terns. 展开更多
关键词 White-winged Black Tern morphometrics wing molt AUSTRALIA feeding ROOSTING
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Demography and dynamics of mountain nyala Tragelaphus buxtoni in the Bale Mountains National Park,Ethiopia 被引量:1
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作者 Yosef MAMO Michelle A.PINARD Afework BEKELE 《Current Zoology》 SCIE CAS CSCD 北大核心 2010年第6期660-669,共10页
We studied the population dynamics of endangered mountain nyala Tragelaphus buxtoni between 2003-2005 in the Bale Mountains National Park. Line-transect sampling and total count methods were used to gather data on dem... We studied the population dynamics of endangered mountain nyala Tragelaphus buxtoni between 2003-2005 in the Bale Mountains National Park. Line-transect sampling and total count methods were used to gather data on demographics and movement patterns. The population's age-group composition was 58% adults, 25% sub-adults, 9% juveniles, 5% calves and 3% unidentified with a female-male sex ratio of 2:1. Population density was found to be significantly different between the two sub-populations (Dinsho Sanctuary and Gaysay/Adelay). A significant difference was found for age-group composition across the two sub-populations except adult females, sub-adult males and calves. The Dinsho sub-population was an isolated group. Separation and containment of the mountain nyala population could have negatively affected their ability to search for habitat requirements and mates from distant areas. The population varied between 830-908 individuals (95% CI), a reduction of 45% from earlier reports. However, the mean population density increased due to contraction of the species' habitat range. We observed a population decrease of 2%-5% per year over the course of our study. Many of the assessed demographic parameters did not significantly change over the three years. This suggests that the decrease in nyala population was not due to random variations in reproduction. Anthropogenic factors such as competition with livestock for forage, habitat encroachment and poaching by the local people might have been partly responsible for the depleted population in our study areas [Current Zoology 56 (6): 660q569, 2010]. 展开更多
关键词 Density DYNAMICS Group size Mountain nyala MOVEMENT Sex ratio
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Sustainable Ecosystem Management versus Economic Exploitation: A Case Study of Lake Naivasha Basin, Kenya
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作者 P.M. Njogu J.J. Kitetu J.M. Keriko 《Journal of Environmental Science and Engineering》 2010年第7期50-55,共6页
Human activities in the Lake Naivasha catchments pose serious ecological threats to sustainable freshwater ecosystem management. The future hangs on the balance of economic exploitation and sustainable watershed conse... Human activities in the Lake Naivasha catchments pose serious ecological threats to sustainable freshwater ecosystem management. The future hangs on the balance of economic exploitation and sustainable watershed conservation. Water abstraction volumes, embedded water/virtual water and chemical analysis of wastewater discharges were assessed. Dissolved oxygen, temperature, Secchi disc depth, benthic organisms' population and pesticides used in the farms were determined. The study found that abstraction exceeds safe water yield, farms have also encroached on the riparian lands and connected to the lake through canals allowing effluents to flow directly to the lake. Benthic community populations are low at points where discharge canals enter the lake, indicating inflow of materials are toxic. Canal samples show high contaminant levels of actions for sustainable exploitation of the resource is essential. compared to other sites. Wise use of the resource and implementation The determination of ecological and water footprints are recommend. 展开更多
关键词 Lake Naivasha AQUATIC ECOSYSTEM PESTICIDES pollution CANALS EFFLUENTS
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Data Regarding Qualitative and Quantitative Composition of Benthic Populations from Musura Bay-Danube Delta, Romania
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作者 Manuela Diana Samargiu Daciana Sava +1 位作者 Dorel Ureche Gabriela Mihaela Pamschiv 《Journal of Environmental Science and Engineering(B)》 2015年第11期569-573,共5页
The present paper refers to some data regarding zoobenthic communities from one of northern lagoon of the Danube delta, known as Musura bay (ROSCI0065, coded as coastal lagoon * 1150). Faunal composition is influen... The present paper refers to some data regarding zoobenthic communities from one of northern lagoon of the Danube delta, known as Musura bay (ROSCI0065, coded as coastal lagoon * 1150). Faunal composition is influenced by the mixture of waters from the Danube and the marine coastal zone. According to monitored areas, there are dominant freshwater or marine invertebrates forming populations that live in the sediments. The aim of this paper is to offer a view of the endopsammic and endopelitic benthic communities from Musura bay, during two years of observations (2013 and 2014) and analyze the seasonal dynamics of invertebrates' populations. Based on quantitative samples collected from infralittoral zone, the paper presents the qualitative structure and a quantitative analyze (abundance, constancy--F%, dominance--D% and Wo%) of invertebrate populations from all taxa (belonging to Foraminifera, Nematoda, Gasteropoda, Oligochaeta, Polychaeta, Ostracoda, Copepoda, Cumacea, Amphipoda and Chironomidae etc.) found in the researched zones, in connection with variation of local ecological conditions. Some considerations regarding the role of invertebrates' populations as food resources for lagoon fishes are done, 展开更多
关键词 Black sea Danube delta Musura bay zoobenthos.
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分段DNA shuffling:一种大分子海藻糖合酶有效的定向进化方法 被引量:2
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作者 刘艳超 王宇凡 +4 位作者 钱柯帆 张峻 肖辰鹏 邢来君 李明春 《微生物学通报》 CAS CSCD 北大核心 2013年第2期362-372,共11页
【目的】红色亚栖热菌(Meiothermus ruber)海藻糖合酶(Trehalose synthase,M-TreS)将麦芽糖转化生成海藻糖只需一步反应,且具有很好的热稳定性及pH耐受性,是潜在的工业生产海藻糖的酶源。为了提高该酶的性能,有必要对其进行定向进化。... 【目的】红色亚栖热菌(Meiothermus ruber)海藻糖合酶(Trehalose synthase,M-TreS)将麦芽糖转化生成海藻糖只需一步反应,且具有很好的热稳定性及pH耐受性,是潜在的工业生产海藻糖的酶源。为了提高该酶的性能,有必要对其进行定向进化。【方法】M-TreS基因(M-treS)大小为2 889 bp。该蛋白质分子本身具有很大的进化空间,但是却不宜进行全长基因Shuffling。分段DNA shuffling是为大分子蛋白质(基因≥2 000 bp)的进化而设计的一种方法。该方法分为三步:(1)用两对引物分别扩增目的基因的上游片段和下游片段;(2)上下游片段各自进行Shuffling;(3)利用重叠延伸PCR连接上下游突变群,建立完整基因的突变文库。【结果】结合易错PCR,通过该方法经一轮进化获得一株酶活力是野生型1.6倍、催化效率是野生型2倍的突变株。序列分析表明,该突变株共有6个位点发生了氨基酸的替代,其中一个来自易错突变,2个来自同源重组,3个为随机突变。【结论】分段DNA shuffling是进化大分子蛋白质的有效方法。 展开更多
关键词 红色亚栖热菌 海藻糖合酶 定向进化 易错PCR 分段DNA SHUFFLING
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宋人书论小札(三) 笔实:黄庭坚的审美理想
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作者 周勋君 《东方艺术》 2016年第12期116-123,共8页
一 黄庭坚大约是历史上第一位在使用文字时不避讳"书家"一词的士人。 在他之前,只有三个人在自己的著录中使用过这一概念:唐贞元年间的韩方明、晚唐的亚栖,以及北宋中期的朱长文。并且,三人使用这一概念统共四次。([1])这其中,... 一 黄庭坚大约是历史上第一位在使用文字时不避讳"书家"一词的士人。 在他之前,只有三个人在自己的著录中使用过这一概念:唐贞元年间的韩方明、晚唐的亚栖,以及北宋中期的朱长文。并且,三人使用这一概念统共四次。([1])这其中,韩方明生平事迹不详,亚栖为一介僧人,唯有朱长文中过进士,却几乎终身隐居不仕, 展开更多
关键词 朱长文 韩方明 亚栖 书论 北宋中期 米芾 贞元 二王 生平事迹 颜鲁公
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SOME BIOLOGICAL ASPECTS AND A UNIQUE HABITAT CHOICE OF DAMSELFLY INDOCNEMIS ORANG FOERSTER(ODONATA:PLATYCNEMIDIDAE)FROM MALAYSIA 被引量:2
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作者 Y.Norma-Rashid 《Entomologia Sinica》 CSCD 2003年第2期115-120,共6页
The hemimetabolous preliminary studies on the life cycle of Indocnemis orang is here reported for the first time, being completed in 6 instars for a minimum duration of 69 days. It can be classified as a stenotopic s... The hemimetabolous preliminary studies on the life cycle of Indocnemis orang is here reported for the first time, being completed in 6 instars for a minimum duration of 69 days. It can be classified as a stenotopic species being sensitive to biotope requirements but having tolerance towards fluctuating water qualities during its developmental process. The need for caution in the interpretation of bioindicator species for habitat assessment and biomonitoring is here both indicated and discussed. 展开更多
关键词 DRAGONFLIES LIFE-HISTORY habitat MALAYSIA MORPHOMETRICS larvae
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Spatially biased dispersal of acorns by a scatter-hoarding corvid may accelerate passive restoration of oak habitat on California's largest island
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作者 Mario B. PESENDORFER T. Scott SILLETT Scott A. MORRISON 《Current Zoology》 SCIE CAS CSCD 2017年第4期363-367,共5页
Scatter hoarding by corvids (crows, jays, magpies, and nutcrackers) provides seed dispersal for many large-seeded plants, including oaks and pines. When hoarding seeds, corvids often choose nonrandom locations throu... Scatter hoarding by corvids (crows, jays, magpies, and nutcrackers) provides seed dispersal for many large-seeded plants, including oaks and pines. When hoarding seeds, corvids often choose nonrandom locations throughout the landscape, resulting in differential survival of seeds. In the context of habitat restoration, such disproportional storing of seeds in areas suitable for germin- ation and establishment can accelerate expansion and recovery of large-seeded tree populations and their associated ecosystems. Here, we investigate the spatial preferences of island scrub jays Aphelocoma insularis during scatter hoarding of acorns (Quercus spp.) on Santa Cruz Island. We use a large behavioral data set on the birds' behavior in combination with seedling surveys and spatial analysis to determine whether 1) island scrub jays disproportionally cache seeds in specific habitat types, and 2) whether the preferred habitat type is suitable for oak regeneration. Our results show that the jays nonrandomly cache acorns across the landscape; they use chaparral and coastal sage scrub disproportionally while avoiding open and grassy areas. The areas used most often for caching were also the areas with the highest oak seedling densities. We discuss the potential role of these findings for the recovery of Santa Cruz Island's oak habitat since the 1980s. 展开更多
关键词 Aphelocoma insularis directional dispersal habitat restoration Quercus spp. scatter hoarding seed dispersal.
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Assessing habitat quality of the mountain nyala Tragelaphus buxtoni in the Bale Mountains, Ethiopia
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作者 Paul H. EVANGELISTA John NORMAN III +1 位作者 Paul SWARTZINKI3,Paul SWARTZINKI3,Paul SWARTZINKI Nicholas E. YOUNG 《Current Zoology》 SCIE CAS CSCD 2012年第4期525-535,共11页
Populations of the endangered mountain nyala Tragelaphus buxtoni are significantly threatened by the loss of critical habitat. Population estimates are tentative, and information on the species' distribution and avai... Populations of the endangered mountain nyala Tragelaphus buxtoni are significantly threatened by the loss of critical habitat. Population estimates are tentative, and information on the species' distribution and available habitat is required for for-mulating immediate management and conservation strategies. To support management decisions and conservation priorities, we integrated information from a number of small-scale observational studies, interviews and reports from multiple sources to define habitat parameters and create a habitat quality model for mountain nyala in the Bale Mountains. For our analysis, we used the FunConn model, an expertise-based model that considers spatial relationships (i.e., patch size, distance) between the species and vegetation type, topography and disturbance to create a habitat quality surface. The habitat quality model showed that approxi- mately 18,610 km^2 (82.7% of our study area) is unsuitable or poor habitat for the mountain nyala, while 2,857 km^2 (12.7%) and 1,026 km^2 (4.6%) was ranked as good or optimal habitat, respectively. Our results not only reflected human induced habitat deg-radation, but also revealed an extensive area of intact habitat on the remote slopes of the Bale Mountain's southern and southeast- ern escarpments. This study provides an example of the roles that expert knowledge can still play in modem geospatial modeling of wildlife habitat. New geospatial tools, such as the FunConn model, are readily available to wildlife managers and allow them to perform spatial analyses with minimal software, data and training requirements. This approach may be especially useful for species that are obscure to science or when field surveys are not practical . 展开更多
关键词 Expert knowledge FunConn model Habitat mapping Land cover Mountain nyala Wildlife conservation
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