血红素铁作为一种铁卟啉类化合物,在医药行业具有很高的药用价值。如作为生物态补铁剂,能克服传统补血剂不能被机体直接吸收和吸收率低的缺点。但其中的 Fe 只有以二价的形式存在才能被人体直接吸收。本文对血红素铁的纯化和制备过程中...血红素铁作为一种铁卟啉类化合物,在医药行业具有很高的药用价值。如作为生物态补铁剂,能克服传统补血剂不能被机体直接吸收和吸收率低的缺点。但其中的 Fe 只有以二价的形式存在才能被人体直接吸收。本文对血红素铁的纯化和制备过程中亚铁的保护进行探讨。展开更多
The efficiency of a new cryoprotectant,GP,for the preservation of Acidithiobacillus ferrooxidans（A.ferrooxidans） strain DC in liquid nitrogen was investigated.The optimal concentration of this new cryoprotectant for...The efficiency of a new cryoprotectant,GP,for the preservation of Acidithiobacillus ferrooxidans（A.ferrooxidans） strain DC in liquid nitrogen was investigated.The optimal concentration of this new cryoprotectant for the maximal viable cell recovery and the highest ferrous ion oxidation activity was determined.The results show that 30%（volume fraction） GP is optimal for the cryopreservation with 84.4% of cells surviving,completely oxidizing ferrous ions within 120 h,and growing to a final density of 5.8×107 cell/mL after 6 d in the culture.Furthermore,the optimal residual GP concentration for viable cell recovery after culture of thawed cells in 9K medium for 6 d is 0.6%（volume fraction）.At this concentration,strain DC completely oxidizes ferrous ions within 108 h and grows to a final cell density of 6.8×107 mL-1.Thus,GP is a simple,effective cryoprotectant for the preservation of A.ferrooxidans strain DC in liquid nitrogen.展开更多
基金Project(2005DKA21208) supported by the R&D Infrastructure and Facility Development Program from the Ministry of Science and Technology of ChinaProject(2010CB630901) supported by the National Basic Research Program of China
文摘The efficiency of a new cryoprotectant,GP,for the preservation of Acidithiobacillus ferrooxidans（A.ferrooxidans） strain DC in liquid nitrogen was investigated.The optimal concentration of this new cryoprotectant for the maximal viable cell recovery and the highest ferrous ion oxidation activity was determined.The results show that 30%（volume fraction） GP is optimal for the cryopreservation with 84.4% of cells surviving,completely oxidizing ferrous ions within 120 h,and growing to a final density of 5.8×107 cell/mL after 6 d in the culture.Furthermore,the optimal residual GP concentration for viable cell recovery after culture of thawed cells in 9K medium for 6 d is 0.6%（volume fraction）.At this concentration,strain DC completely oxidizes ferrous ions within 108 h and grows to a final cell density of 6.8×107 mL-1.Thus,GP is a simple,effective cryoprotectant for the preservation of A.ferrooxidans strain DC in liquid nitrogen.
