The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was...The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.展开更多
目的:用气相色谱内标法测定蛹油α-亚麻酸乙酯制剂中α-亚麻酸乙酯的含量。方法:采用气相色谱法,DEGS为固定相,涂布浓度为10％,载体为Chromosorb W AW DMCS80-100目的气相柱。结果:平均回收率为97.87％,RSD为1.52％。结论:该法操作简便...目的:用气相色谱内标法测定蛹油α-亚麻酸乙酯制剂中α-亚麻酸乙酯的含量。方法:采用气相色谱法,DEGS为固定相,涂布浓度为10％,载体为Chromosorb W AW DMCS80-100目的气相柱。结果:平均回收率为97.87％,RSD为1.52％。结论:该法操作简便、准确、重现性、稳定性好,可作为蛹油α-亚麻酸乙酯的含量测定方法。展开更多
文摘The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.
文摘目的:用气相色谱内标法测定蛹油α-亚麻酸乙酯制剂中α-亚麻酸乙酯的含量。方法:采用气相色谱法,DEGS为固定相,涂布浓度为10％,载体为Chromosorb W AW DMCS80-100目的气相柱。结果:平均回收率为97.87％,RSD为1.52％。结论:该法操作简便、准确、重现性、稳定性好,可作为蛹油α-亚麻酸乙酯的含量测定方法。
