The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was...The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.展开更多
[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba lea...[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba leaves were done and the volatile components of hexane extractives of M.alba leaves with the strongest antibacterial activity were analyzed by gas chromatography-mass spectrometry（GC-MS）.[Result]The results of inhibitory effect of hexane extractives of M.alba leaves on Staphylococcus aureus and Escherichia coli showed that hexane extractives of M.alba leaves collected in June,July and August had antibacterial activity against S.aureus and had no antibacterial effect against E.coli.Furthermore,hexane extractives of M.alba leaves collected in June had the strongest antibacterial activity against S.aureus（inhibition diameter 10.95 mm）.The hexane extractives of M.alba leaves collected in June was analyzed by GC-MS,the main volatile components were tetradecane（16.76%）,dodecane（13.20%）,diisobutyl phthalate（10.26%）,decane（9.10%）,hexadecane（8.71%）,linolenyl alcohol（7.25%）,octadecane（5.88%）,eicosane（3.26%）,dibutyl phthalate（2.59%）.[Conclusion]Linolenyl alcohol was the potential antibacterial compound.展开更多
文摘The effects of several lipases on oleyl linolenate synthesis were investigated. The lipase having the highest esterification activity was screened by our laboratory and the yeast which produced this kind of lipase was Trichosporon , named as Y 11. Several factors which influenced oleyl linolenate synthesis were studied. The optimum temperature for the esterification was 30 ℃ and the reaction could be carried out in the temperature range of 4100 ℃, the optimum pH was 8.0, the optimum substrate concentration was 0.5 mol/L. The esterification rate could amount to 85% after 22 h and could further reach 95.2% if the water produced in the reaction was removed in time. Among 11 kinds of organic solvents compared, the esterification rate in n hexane was the highest and that in dimethylsulfoxide was the lowest, the former was ten times higher than the latter.
基金Supported by the Forestry Department of Heilongjiang Province Science and Technology Promotion Project(01043208003)the Project of Science and Technology Department of Heilongjiang Province(LC07C27)~~
文摘[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba leaves were done and the volatile components of hexane extractives of M.alba leaves with the strongest antibacterial activity were analyzed by gas chromatography-mass spectrometry（GC-MS）.[Result]The results of inhibitory effect of hexane extractives of M.alba leaves on Staphylococcus aureus and Escherichia coli showed that hexane extractives of M.alba leaves collected in June,July and August had antibacterial activity against S.aureus and had no antibacterial effect against E.coli.Furthermore,hexane extractives of M.alba leaves collected in June had the strongest antibacterial activity against S.aureus（inhibition diameter 10.95 mm）.The hexane extractives of M.alba leaves collected in June was analyzed by GC-MS,the main volatile components were tetradecane（16.76%）,dodecane（13.20%）,diisobutyl phthalate（10.26%）,decane（9.10%）,hexadecane（8.71%）,linolenyl alcohol（7.25%）,octadecane（5.88%）,eicosane（3.26%）,dibutyl phthalate（2.59%）.[Conclusion]Linolenyl alcohol was the potential antibacterial compound.
