The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable perf...The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U·ml^-1 adsorbent, and two active peaks were eluted respectively at 0.15mol·L^-1 and 0.3mol·L^-1 （NH4）2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.展开更多
This paper devises the theory of universal residual intersection. The mainresult is that in an extension of R of the form S=R(X)=R mR , there is an s -residual intersection URI(s;I) of IS , it has th...This paper devises the theory of universal residual intersection. The mainresult is that in an extension of R of the form S=R(X)=R mR , there is an s -residual intersection URI(s;I) of IS , it has the properties that given any s -residual intersection J of I in R , URI(s;I) is essentially a deformation of J , and is the localization RI(s;I) mR of the generic s -residual intersection RI(s;I) of I .展开更多
基金the Natural Science Foundation of Ningbo （No.2005A610018）.ACKN0WLEDGEMENTS The authors would like to thank Mr. Qi Nan for taking part in this study, and for financial support by the Natural Science Foundation of Ningbo. Discussion with Dr. Lin Dong-Qiang has been helpful and is appreciated.
文摘The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U·ml^-1 adsorbent, and two active peaks were eluted respectively at 0.15mol·L^-1 and 0.3mol·L^-1 （NH4）2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.
文摘This paper devises the theory of universal residual intersection. The mainresult is that in an extension of R of the form S=R(X)=R mR , there is an s -residual intersection URI(s;I) of IS , it has the properties that given any s -residual intersection J of I in R , URI(s;I) is essentially a deformation of J , and is the localization RI(s;I) mR of the generic s -residual intersection RI(s;I) of I .
