To determine the effects of HOE 694, a new and potent Na＋- H＋ exchanger blocker, on transient inward current (Iti) and Na＋- Ca2＋ exchange during hypoxia- reoxygenation in guinea pig cardiomyocytes. Methods. Cardio...To determine the effects of HOE 694, a new and potent Na＋- H＋ exchanger blocker, on transient inward current (Iti) and Na＋- Ca2＋ exchange during hypoxia- reoxygenation in guinea pig cardiomyocytes. Methods. Cardiomyocytes were isolated from adult guinea pig ventricle. Experiment was performed in an experimental chamber that allowed the cells to be exposed to a sufficiently low O2 pressure. The cells were subjected to hypoxia and reoxygenation. The ionic currents were studied with patch clamp technique. Results. In the absence of HOE 694, hypoxia- reoxygenation induced Iti in 12 of 15 experiments; but in cardiomyocytes pretreated with HOE 694 (10～ 50μ mol/L), the incidence of Iti observed during reoxygenation was reduced to 5 of 11 experiments and 3 of 10 experiments, P Conclusions. Blockade of the Na＋- H＋ exchange by HOE 694 could reduce Ca2＋ overload upon hypoxia- reoxygenation, and inhibition of Na＋- H＋ exchange may also indirectly decrease Na＋- Ca2＋ exchange activity during hypoxia.展开更多
Aimed at the shortcoming that the loss of low-frequency information of alternating current millimeter-wave radiometer signal, relevance vector machine (RVM) algorithm is used to compensate the lost component in discre...Aimed at the shortcoming that the loss of low-frequency information of alternating current millimeter-wave radiometer signal, relevance vector machine (RVM) algorithm is used to compensate the lost component in discrete cosine transform (DCT) domain, and through inverse discrete cosine transform (IDCT) we can receive the compensated signal. RVM exploits Bayesian learning framework, which has dramatically fewer kernel functions than comparative support vector machine. So that accurate prediction models can be acquired. Experimental results also show that this method can obtain good compensation effect.展开更多
基金This work was supported by Leading Specialty Funding of Shanghai, Grant No.94- III- 001.
文摘To determine the effects of HOE 694, a new and potent Na＋- H＋ exchanger blocker, on transient inward current (Iti) and Na＋- Ca2＋ exchange during hypoxia- reoxygenation in guinea pig cardiomyocytes. Methods. Cardiomyocytes were isolated from adult guinea pig ventricle. Experiment was performed in an experimental chamber that allowed the cells to be exposed to a sufficiently low O2 pressure. The cells were subjected to hypoxia and reoxygenation. The ionic currents were studied with patch clamp technique. Results. In the absence of HOE 694, hypoxia- reoxygenation induced Iti in 12 of 15 experiments; but in cardiomyocytes pretreated with HOE 694 (10～ 50μ mol/L), the incidence of Iti observed during reoxygenation was reduced to 5 of 11 experiments and 3 of 10 experiments, P Conclusions. Blockade of the Na＋- H＋ exchange by HOE 694 could reduce Ca2＋ overload upon hypoxia- reoxygenation, and inhibition of Na＋- H＋ exchange may also indirectly decrease Na＋- Ca2＋ exchange activity during hypoxia.
基金National Defence Foundation under Grant No.9140A05070107BQ0204
文摘Aimed at the shortcoming that the loss of low-frequency information of alternating current millimeter-wave radiometer signal, relevance vector machine (RVM) algorithm is used to compensate the lost component in discrete cosine transform (DCT) domain, and through inverse discrete cosine transform (IDCT) we can receive the compensated signal. RVM exploits Bayesian learning framework, which has dramatically fewer kernel functions than comparative support vector machine. So that accurate prediction models can be acquired. Experimental results also show that this method can obtain good compensation effect.
