Isolation and Identification of Submergence-induced Genes in Maize (Zea mays) Seedlings by Suppression Subtractive Hybridization

To investigate the expression profile of maize genes induced by submergence, a subtracted cDNA library of maize seedling roots was constructed using suppression subtractive hybridization (SSH). The cDNA of maize seedling roots treated with submergence (ST) was used as tester and what from untreated roots (UT) as driver. Products of the secondary PCR from the forward subtraction were cloned into T/A vector and transferred into Escherichia coli strain JM10B by electroporation. Four hundred and eight randomly chosen transformants carrying cDNA fragments were screened with PCR-Select Deferential Screening Kit. One hundred and eighty-four cDNA clones were identified as, submergence specifically induced or highly expressed. After sequencing and removing redundant cDNAs, we got 95 submergence-induced cDNA clones. Of the 95 cDNA clones, 68 contain the regions with 60%-90% identity to their homolog in GenBank, 21 are expected to be novel genes, only 6 correspond to the published maize sequences.

Formation of the entorhino-hippocampal pathway: a tracing study in vitro and in vivo

Objective The entorhino-hippocampal pathway is the major excitatory input from neurons of the entorhinal cortex on both ipsilateral and contralateral hippocampus/dentate gyrus. This fiber tract consists of the alvear path, the perforant path and a crossed commissural projection. In this study, the histogenesis and development of the various subsets of the entorhino-hippocampal projection have been investigated. Methods Dil, DiO and fast blue tracing as well as anti-calretinin immunocytochemistry were carried out with prenatal and postnatal rats at different ages. Results The alvear path and the commissural pathway started to develop as early as embryonic day （E） 16, while the first perforant afferents reached the stratum lacunosum-moleculare of the hippocampus at E 17 and the outer molecular layer of dentate gyrus at postnatal day （P） 2, respectively. Retrograde tracing with DiI identified entorhinal neurons in layer II to IV as the origin of entorhino-hippocampal pathway. Furthermore, anti-calretinin immunocytochemistry revealed transitory Cajal- Retzius （CR） cells in the stratum lacunosum-moleculare of the hippocampus from as early as E 16. DiI labeling of entorhinal cortex fibers and combined calretinin-immunocytochemistry showed a close association between CR cells and entorhinal afferents. Conclusion The subsets of entorhino-hippocampal pathway appear in the developmental hippocampus during El6 - P2. The temporal and spatial relationship between CR cell and perforant afferent suggests the role of this cell type as a guiding cue for entorhinal afferents at early cortical development.

Tribocorrosion behaviors of Ti-6Al-4V and Monel K500 alloys sliding against 316 stainless steel in artificial seawater

The tribocorrosion behaviors of Ti-6Al-4V and Monel K500 alloys sliding against 316 stainless steel were investigated using a ring-on-block test rig in both artificial seawater and distilled water. It is found that friction coefficients are in general larger in distilled water compared with seawater. The wear losses of Ti-6Al-4V and Monel K500 alloys are larger in seawater compared with distilled water. The mechanical action can destroy the passive film and increase the corrosion rate. The synergism effect between corrosion and wear occurs. The synergism action between corrosion and wear is related to the corrosion rate and with the increase of corrosion rate, the synergism becomes more important. 316 stainless steel suffers severe wear sliding against Monel K500 alloy compared with sliding against Ti-6Al-4V alloy in both distilled water and seawater.

Characteristics of Deposition of Inosine Monophosphate(IMP) and Intramuscular Fat(IMF) in Muscles of Jinghai Yellow Chicken and Its Crossbreeds

[Objective] This study aimed to investigate the characteristics of deposition of inosine monophosphate （IMP） and intramuscular fat （IMF） in muscles of Jinghai yellow chickens and its crossbred.[Method] The characteristics of IMP and IMF deposition of 112-day-old Jinghai yellow chickens （J×J） and its two different 70-day- old crossbreeds （J×B and B×B） were analyzed. The IMP content in breast muscle and leg muscle were determined by HPLC. [Result] The contents of IMP and cor- rected inosine monophosphate （IMPc） in breast muscle were significantly or ex- tremely significantly higher than that in leg muscle of the chickens in the three groups whether in male or female chickens （P〈0.05 or P〈0.01）. There were no sig- nificant difference in the contents of IMP and IMPc between hens and roosters （P〉 0.05）. The fresh degree of breast muscle and leg muscle was 96,11%-98.16% and 87.22%-93.07%, respectively. And the fresh degree of breast muscle was higher than that of leg muscle. In the three groups, the IMF content in leg muscle was significantly higher than that in breast muscle whether in male or female chickens （P〈0.05）. The contents of IMF in breast muscle and leg muscle were 0.36%-0.75% and 1.84%-2.38%, respectively. The iMP content in breast muscle of chickens in Bx J group was extremely significantly higher than that in breast muscle of chickens in JxJ group （P〈0.01）, but the contents of IMPc and iMF of breast muscle and leg muscle of the chickens in the three groups had no significant difference （P〉0.05）. [Conclusion] To sum up, the freshness and flavor significantly differ between the breast muscle and leg muscle of chickens, but show no significant difference among the three groups.

省政府办公厅关于转发省交通厅省安监局江苏海事局全省渡口渡船安全管理专项整治实施方案的通知

各市、县人民政府,省各委、办、厅、局,省各直属单位:省交通厅、省安监局、江苏海事局制定的《全省渡口渡船安全管理专项整治实施方案》已经省政府同意,现转发给你们,请认真贯彻执行。为规范渡口渡船安全管理,确保人民群众生命财产安全,根据交通部、国家安全生产监督管理总局《关于开展渡口渡船专项整治规范渡口渡船安全管理的意见》(交海发[2005]324号)和《关于印发渡口渡船安全管理专项整治实施方案的通知》(交海发[2005]412号)精神,结合我省实际。

“海丝”复兴

这是一条神奇的海路。它自秦汉之际兴起,在历史延伸中不断拓展为交通贸易的黄金路线。它自中国东南沿海,穿过南中国海,进入印度洋、波斯湾,远及东非、欧洲,构成四通八达的交通网络。＂舶交海中,不知其数＂,蔚成中国与外国商业贸易的大动脉。它就是海上丝绸之路。隋唐时期,运送的大宗货物多是丝绸,人们把它称作＂海上丝绸之路＂;宋元时期,瓷器渐成主要出口货物,它被叫作＂陶瓷之路＂;明代,

交通运输部 国家安全监管总局关于公布2013年度公路水运建设“平安工程”冠名项目的通知

交海发[2014]74号各省、自治区、直辖市、新疆生产建设兵团交通运输厅(局、委)、安全生产监督管理局,上海市、天津市交通运输和港口管理局,天津市市政公路管理局,长江航务管理局,长江口航道管理局:根据《交通运输部安全监管总局关于组织公路水运建设项目平安工程冠名工作的通知》(交质监发[2012]639号)有关要求,经项目申报、省级交通运输和安全监管部门的联合推荐、专家评审、媒体公示,以及交通运输部、国家安全监管总局有关部门联合审定,

浙江省人民政府办公厅关于印发浙江省船舶排放控制区实施方案的通知

浙政办发[2016]37号各市、县(市、区)人民政府,省政府直属各单位:《浙江省船舶排放控制区实施方案》已经省政府同意,现印发给你们,请认真贯彻实施。2016年3月30日浙江省船舶排放控制区实施方案为贯彻落实《中华人民共和国大气污染防治法》,改善环境空气质量,促进航运绿色发展,根据交通运输部《关于印发珠三角、长三角、环渤海(京津冀)水域船舶排放控制区实施方案的通知》(交海发[2015]177号)

交通运输部关于加强水上污染应急工作的指导意见

交海发[2010]366号各省、自治区、直辖市、新疆生产建设兵团交通运输厅(局、委),天津市、上海市交通运输和港口管理局,各直属海事局:近期,国内外连续发生水上溢油和化学品污染事故,对水域生态环境造成了严重损害,特别是墨西哥湾"4·20"

Technology for Enzymolysis of Jellyfish Brain Protein by Bromelain

[Objective] This study aimed to investigate the optimal technology for en- zymolysis of jeUyfish brain protein by bromefain. [Method] Effects of enzymolysis temperature, the amount of added enzyme, solid/liquid ratio, enzymolysis time and pH on the enzymolysis of total protein were investigated through single factor ex- periments, and some technical parameters were optimized by orthogonal experi- ments. [Result] The results revealed that enzymolysis temperature of 55℃, the amount of added enzyme of 4 000 U/g, solid/liquid ratio of 1：4, the amount of added enzyme time for 5 h and pH 7 were the optimal combination of parameters for the enzymolysis; the average degree of hydrolysis obtained from the verification tests was 36.9%, indicating that the optimized results of the orthogonal experiment were credible. [Conclusion] This study provides the optimized conditions reliable theo- retical foundation and technical support for the exploitation and utilization of jellyfish resources as medicines or functional food in China.

Yield trait improvement of bay scallops following complete diallel crosses between different scallop stocks

This study describes the complete diaUel hybridization between newly introduced bay scallop stock （W） from Canada and local commercial stock （D） grown under laboratory conditions, in China. Larval survival and growth during all life stages （larvae, spat, and adult） were compared among hybrid （DW, WD） and purebred （DD, WW） populations. Significant heterosis was detected for survival during the larval stage （〉 1% of the mid-parent values）. The mean heterosis （Hm） varied in growth throughout the life span. More than 50% of the Hm values were positive and negative in the DW and WD groups, respectively. The influence of maternal effects and mating types （intrapopulation vs. interpopulation crosses） on growth for all life stages was not consistent. Larval survival did not differ significantly （P〉0.05） with maternal effect or mating type. In the harvest stage, shell length （SL）, shell height （SH）, shell width （SW）, and total weight （TW） were larger in the hybrid compared with the inbred groups. Positive Hm values were observed in SL （1.5%）, SW （5.8%）, and TW （12.3%）, and were more significant in the DW groups （6.1%, 4.5%, 6.8%, and 27.2%）. These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.

Influence of Reclamation Works on the Marine Environment in a Semi-Enclosed Bay

The flow and seawater exchange rates have been predicted using a two-dimensional numerical model and a Lagrangian method for a semi-enclosed shallow bay where reclaiming and dredging works are scheduled. The wind effect on the flow and material transport has been emphasized, and a thirty-year mean value of wind has been considered in the numerical simulation. As a whole, even after the reclaiming and dredging are conducted, the flow pattern looks similar to the original state. However, velocity variations up to 20% to 100% appear in the vicinity of the construction area. In the case of summcr wind forcing, the seawater exchange rate increases from 71.6% to 82.9% after the reclaiming and dredging, as indicated by a particle-tracking method. On the contrary, in the case of winter wind forcing, thc seawater cxchange rate appears to be 97.2% under natural conditions but decrcases slightly to 93.2% aftcr the rcclaiming and dredging. Thus, the wind forcing plays an important role in controlling the seawater exchangc rates. The seawater cxchange rate is further improved by 15% if the dredging is simultaneously carried out with the reclaiming. This suggests that the dredging can be an effective means to mitigate the variation of flow.

Distribution of Dissolved Inorganic Carbon (DIC) and Its Related Parameters in Seawater of the North Yellow Sea and off the Qingdao Coast in October, 2007

Data on the distribution of dissolved inorganic carbon (DIC) were obtained from two cruises in the North Yellow Sea (NYS) and off the Qingdao Coast (QC) in October, 2007. Carbonate parameters were calculated. The concentrations of DIC are from 1.896-2.229 mmolL-1 in the NYS and from 1.939-2.032 mmolL-1 off the QC. In the southwest of the NYS, DIC in the upper layers decreases from the north of the SP (Shandong Peninsula) shelf to the center of the NYS; whereas in the lower layers DIC increases from the north of the SP shelf to the center of the NYS and South Yellow Sea. In the northeast of the NYS, DIC in all layers in- creases from the YR (Yalu River) estuary to the centre of the NYS. The distribution of DIC in NYS can be used as an indicator of Yellow Sea Cold Water Mass (YSCWM). Air-sea CO2 fluxes were calculated using three models and the results suggest that both the NYS and the QC waters are potential sources of atmospheric CO2 in October.

Enhanced CT and CT virtual endoscopy in diagnosis of heterotopic pancreas

AIM:To improve the diagnosis of heterotopic pancreas by the use of contrastenhanced computed tomography(CT)imaging and CT virtual endoscopy(CTVE).METHODS:A total of six patients with heterotopic pancreas,as confirmed by clinical pathology and immunohistochemistry in the Sixth Affiliated People's Hospital of Shanghai Jiao Tong University,Shanghai,China,were included.Nonenhanced CT and enhanced CT scanning were performed,and the resulting images were reviewed and analyzed using threedimensional postprocessing software,including CTVE.RESULTS:Four males and two females were enrolled.Several heterotopic pancreas sites were involved;three occurred in the stomach,including the gastric antrum(n =2)and lesser curvature(n=1),and two were in the duodenal bulb.Only one case of heterotopic pancreas lesion occurred in the mesentery.Four cases had a solid yet soft tissue density that had a homogeneous pattern when viewed by enhanced CT.Additionally,their CT values were similar to that of the pancreas.The ducts of the heterotopic pancreas tissue,one of the characteristic CT features of heterotopic pancreas tissue,were detected in the CT images of two patients.CTVE images showed normal mucosa around the tissue,which is also an important indicator of a heterotopic pancreas.However,none of the CTVE images showed the typical signs of central dimpling or umbilication.CONCLUSION:CT,enhanced CT and CTVE techniques provide useful information about the location,growth pattern,vascularity,and condition of the gastrointestinal wall around heterotopic pancreatic tissue.

Characteristics of water exchange in the Luzon Strait during September 2006

The Luzon Strait is the only deep channel that connects the South China Sea(SCS) with the Pacific.The transport through the Luzon Strait is an important process influencing the circulation,heat and water budgets of the SCS.Early observations have suggested that water enters the SCS in winter but water inflow or outflow in summer is quite controversial.On the basis of hydrographic measurements from CTD along 120° E in the Luzon Strait during the period from September 18 to 20 in 2006,the characteristics of temperature,salinity and density distributions are analyzed.The velocity and volume transport through the Luzon Strait are calculated using the method of dynamic calculation.The major observed results show that water exchanges are mainly from the Pacific to the South China Sea in the upper layer,and the flow is relatively weak and eastward in the deeper layer.The net volume transport of the Luzon Strait during the observation period is westward,amounts to about 3.25 Sv.This result is consistent with historical observations.

Numerical Study of Viscous Wave-Making Resistance of Ship Navigation in Still Water

The prediction of a ship＇s resistance especially the viscous wave-making resistance is an important issue in CFD applications. In this paper, the resistances of six ships from hull 1 to hull 6 with different hull forms advancing in still water are numerically studied using the solver naoe-FOAM-SJTU, which was developed based on the open source code package OpenFOAM. Different components of the resistances are computed and compared while considering two speed conditions （12 kn and 16 kn）. The resistance of hull 3 is the smallest while that of hull 5 is the largest at the same speed. The results show hull 3 is a good reference for the design of similar ships, which can provide some valuable guidelines for hull form optimization.

Water exchange and circulation structure near the Luzon Strait in early summer

Using hydrographic data covering large areas of ocean for the period from June 21 to July 5 in 2009, we studied the circulation structure in the Luzon Strait area, examined the routes of water exchange between the South China Sea （SCS） and the Philippine Sea, and estimated the volume transport through Luzon Strait. We found that the Kuroshio axis follows a e-shaped path slightly east of 121°E in the upper layer. With an increase in depth, the Kuroshio axis became gradually farther from the island of Luzon. To study the water exchange between the Philippine Sea and the SCS, identification of inflows and outflows is necessary. We first identified which flows contributed to the water exchange through Luzon Strait, which differs from the approach taken in previous studies. We determined that the obvious water exchange is in the section of 121°E. The westward inflow from the Philippine Sea into the SCS is 6.39 Sv in volume, and mainly in the 100-500 m layer at 19.5°-20°N （accounting for 4.40 Sv）, while the outflow from the SCS into the Philippine Sea is concentrated in the upper 100 m at 19°-20°N and upper 400 m at 21°-21.5°N, and below 240 m at 19°-19.5°N, accounting for 1.07, 3.02 and 3.43 Sv in volume transport, respectively.

Localization and the Chinese Overseas： Acculturation, Assimilation, Hybridization, Creolization, and Identification

Based on materials on the localized Chinese overseas, including the Melaka Babas, who are mostly Malay-speaking Chinese, this article reflects on the use of such terms as acculturation and assimilation, as well as hybridization and creolization, in relation to highly localized Chinese. All these concepts are seen as different ways of describing cultural formation in transcultural context. In particular, the relevance of using creolization to refer to the kind of creative process of cultural formation beyond its original usage in the Caribbean is discussed. This results in the identification of fragmented creolization as in the case of the Caribbean and a rooted creolization as in the case of the Babas. The author shall first discuss the issues of assimilation and integration, followed by hybridization and creolization. This is followed by the discussion on localization of Chinese overseas and identity. The concluding section provides some remarks on the concepts reviewed, and three main categories of acculturated Chinese are identified, namely, Chinese who are linguistically assimilated but still observe major Chinese traditions, Chinese who are so acculturated to the mainstream society that they hardly practice Chinese traditions, and Chinese who are both highly localized and highly mixed “racially”.

Benthic nutrient recycling in shallow coastal waters of the Bohai Sea

Sediment-water fluxes of N and P species in the Bohai Sea were investigated in Septem-ber-October 1998 and April-May 1999. The benthic fluxes of nutrient species were determined by incubating sediment core samples with bottom seawater bubbled with air or nitrogen. -2NO,+4NH, dissolved organic nitrogen (DON) and phosphorus (DOP), total dissolved nitrogen (TDN) and phosphorus (TDP), and -34PO showed a net exchange flux from seawater to sediment, while -3NO, dissolved inorganic nitrogen (DIN) and -23SiO were released from sediment to seawater in the Bohai Sea. Sediment-water nutrient exchange increases DIN and reduces the phosphorus load in the Bohai Sea. The release of silicate from sediment to overlying seawater reduces potential silicate limitation of primary production resulted from decrease of riverine discharge. The exchange flux of nutrients showed no obvious seasonal variation. The present study showed that the concentrations and composition of nutrients in the water column were affected by suspended sediment, and that not all the exchangeable phosphate in sediment could be released via sediment resuspension.

Research on the coefficient of sound absorption in turbid water

China’s coastal waters are turbid and the properties of the seabed are complex. This negatively impacts the performance of underwater detection equipment. The properties of sound absorption in turbid water are not well understood. In this paper, the coefficient of sound absorption in turbid water was measured by the reverberation technique. All work was done in a reverberation barrel made of seamless aluminum. First, pure water was poured into the reverberation barrel and its reverberation time measured. Next, various concentrations of turbid water were poured into the barrel and their reverberation time measured. After all data had been gathered, the coefficient of sound absorption in turbid water of different concentrations was calculated. From this we determined a law of sound absorption in turbid water as summarized in the paper.