期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
交联键合型聚丙烯酰胺毛细管电泳涂层柱的快速制备及评价 被引量:9
1
作者 康经武 陆豪杰 +1 位作者 梁冰 欧庆瑜 《分析化学》 SCIE EI CAS CSCD 北大核心 2000年第10期1189-1193,共5页
建立了一种简单快速制备交联键合聚丙烯酰胺涂层毛细管电泳柱的方法。在这一方法中,未经处理的毛细管柱直接用含有一定比例的丙烯酰胺、交联剂甲叉基双丙烯酰胺、硅烷化试剂乙烯基三乙氧基硅氧烷以及引发剂偶氮二异丁氰的丙酮溶液进行... 建立了一种简单快速制备交联键合聚丙烯酰胺涂层毛细管电泳柱的方法。在这一方法中,未经处理的毛细管柱直接用含有一定比例的丙烯酰胺、交联剂甲叉基双丙烯酰胺、硅烷化试剂乙烯基三乙氧基硅氧烷以及引发剂偶氮二异丁氰的丙酮溶液进行动态涂渍,再经热处理使单体的交联和聚合物在毛细管壁上的键合反应同时发生。所制柱能够有效地抑制电渗流和蛋白质在毛细管壁上的吸附。碱性蛋白在 pH 4.0的缓冲液中分离时,获得的平均分离柱效为 9.1×105理论塔板数/米。酸性蛋白在 pH 8.5的缓冲液中获得的分离柱效为 2.0×104理论塔板数/米。酸碱性蛋白质的迁移时间重现性较好,并且所制柱在 pH 2.5~ 8.5的范围内表现了较好的稳定性。 展开更多
关键词 交联键合涂层柱 毛细管电泳 蛋白质 分离
下载PDF
脱氢枞胺键合交联聚苯乙烯树脂的制法和应用 被引量:2
2
作者 何曼 陆进华 沈德渊 《化工时刊》 CAS 2002年第12期25-29,共5页
分3个步骤制备脱氢枞胺键合交联聚苯乙烯树脂:醚化反应、双键环氧化和脱氢枞胺与环氧基开环反应。为了提高树脂中功能基的转化率,针对每一步合成做了正交实验,得出了最佳工艺条件。采用红外光谱对每一步合成产物进行结构表征。并用脱氢... 分3个步骤制备脱氢枞胺键合交联聚苯乙烯树脂:醚化反应、双键环氧化和脱氢枞胺与环氧基开环反应。为了提高树脂中功能基的转化率,针对每一步合成做了正交实验,得出了最佳工艺条件。采用红外光谱对每一步合成产物进行结构表征。并用脱氢枞胺键合交联聚苯乙烯树脂进行对映体分离的初步应用。 展开更多
关键词 脱氢枞胺 交联 交联 聚苯乙烯树脂 手性固定相 醚化 环氧化
下载PDF
阿拉伯木聚糖对小麦面筋蛋白的作用机理研究 被引量:13
3
作者 李娟 王莉 +1 位作者 李晓瑄 陈正行 《粮食与饲料工业》 CAS 北大核心 2012年第1期39-41,共3页
以全麦面包在加工中遇到的问题为研究切入点,对阿拉伯木聚糖凝胶的形成、阿拉伯木聚糖与小麦面筋蛋白的相互作用等机理进行综述。由于阿拉伯木聚糖凝胶阻碍了小麦面筋蛋白网状结构的形成,使全麦面包的烘焙体积较白面包的体积偏小,这是... 以全麦面包在加工中遇到的问题为研究切入点,对阿拉伯木聚糖凝胶的形成、阿拉伯木聚糖与小麦面筋蛋白的相互作用等机理进行综述。由于阿拉伯木聚糖凝胶阻碍了小麦面筋蛋白网状结构的形成,使全麦面包的烘焙体积较白面包的体积偏小,这是导致全麦面包烘焙品质差的主要原因。 展开更多
关键词 阿拉伯木聚糖 小麦面筋蛋白 氧化胶凝 交联键合
下载PDF
COMPATIBILITY OF POLY (STYRENE-B-2-VINYL PYRIDINE)/IONOMER BLENDS BASED ON ION-LIGAND INTERACTION 被引量:1
4
作者 GUO Tianying HAO Guangjie +1 位作者 SONG Moudao ZHANG Banghua 《Chinese Journal of Reactive Polymers》 2002年第1期26-31,共6页
In this work, DSC and SEM studies indicate that ion-ligand interaction can be utilized to enhance the interaction of poly (styrene-block-2-vinyl pyridine)[P (S-b-2VP)] and polyethylene based ionomer (Surlyn). The comp... In this work, DSC and SEM studies indicate that ion-ligand interaction can be utilized to enhance the interaction of poly (styrene-block-2-vinyl pyridine)[P (S-b-2VP)] and polyethylene based ionomer (Surlyn). The compatibility for this blending system can be improved by this special interaction and 20/80 wt is the optimum blending composition with good compatibility. FTIR results further certify that strong interactions exist in the blending system. 展开更多
关键词 Diblock copolymer IONOMER BLENDING Compatibility
下载PDF
Surface-imprinted polymers in microfluidic devices 被引量:4
5
作者 SCHIRHAGL Romana ZARE Richard N. 《Science China Chemistry》 SCIE EI CAS 2012年第4期469-483,共15页
Molecularly imprinted polymers are generated by curing a cross-linked polymer in the presence of a template. During the curing process, noncovalent bonds form between the polymer and the template. The interaction site... Molecularly imprinted polymers are generated by curing a cross-linked polymer in the presence of a template. During the curing process, noncovalent bonds form between the polymer and the template. The interaction sites for the noncovalent bonds become "frozen" in the cross-linking polymer and maintain their shape even after the template is removed. The resulting cavities reproduce the size and shape of the template and can selectively reincorporate the template when a mixture containing it flows over the imprinted surface. In the last few decades the field of molecular imprinting has evolved from being able to selectively capture only small molecules to dealing with all kinds of samples. Molecularly imprinted polymers (MIPs) have been generated for analytes as diverse as metal ions, drug molecules, environmental pollutants, proteins and viruses to entire cells. We review here the relatively new field of surface imprinting, which creates imprints of large, biologically relevant templates. The traditional bulk imprinting, where a template is simply added to a prepolymer before curing, cannot be applied if the analyte is too large to diffuse from the cured polymer. Special methods must be used to generate binding sites only on a surface. Those techniques have solved crucial problems in separation science as well as chemical and biochemical sensing. The implementation of imprinted polymers into microfluidic chips has greatly improved the applicability of microfluidics. We present the latest advances and different approaches of surface imprinting and their applications for microfluidic devices. 展开更多
关键词 molecular imprinting microfluidic devices surface imprinting bioanalysis separation sensors
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部