The conidial tolerance of Metarhizium anisopliae var.anisopliae isolate ARSEF 2575 to UV-B irradiation is greatly influenced by growth-environment alterations.In this review,we report high variability in conidial UV-B...The conidial tolerance of Metarhizium anisopliae var.anisopliae isolate ARSEF 2575 to UV-B irradiation is greatly influenced by growth-environment alterations.In this review,we report high variability in conidial UV-B tolerance in response to altered culture conditions.Conidia produced on insect cadavers[Zophobas morio(Coleoptera)or Galleria mellonella(Lepidoptera)] had low tolerance to UV-B radiation;and conidia produced on potato dextrose agar supplemented with yeast extract(PDAY)had medium UV-B tolerance;whereas conidia produced on a minimal medium without any carbon source(MM),on MM with a non-preferred carbon source such as lactose(=MML),on PDAY plus 1 M NaCl or KCl,or PDBY with high alkalinity had the highest UV-B tolerances.All of the above conditions that induced high UV-B tolerance,however,also greatly reduced conidial production.Comparisons between stress tolerance and conidial production,particularly with conidia produced under osmotic and nutritive stress,point out that the benefits of producing very tolerant conidia have the enormous cost of low conidial production.Growth under visible light also greatly improved conidial UV-B tolerance,but light did not negatively influence conidial production.Therefore,culture on rich media under light is proposed as the most promising approach to producing conidia with improved UV-B tolerance for biological control of pest insects in agriculture.展开更多
As a well known DNA photolesion product, the special UV induced pyrimidine dimmer called spore photoproduct （SP）, has aroused strong research interests. The SP formation was reported solely between two adjacent thym...As a well known DNA photolesion product, the special UV induced pyrimidine dimmer called spore photoproduct （SP）, has aroused strong research interests. The SP formation was reported solely between two adjacent thymidine residues. It remains unclear in pervious experimental observations why there is an absence of the cytosine-derived SP-like photoprod- uct formation at the cytosine containing DNA strand, although the cytosine residue holds great similarity to thymine in terms of molecular structure. From a theoretical perspec- tive, we have explored this issue in this work by means of density functional theory at the B3LYP/6-311＋＋G（d,p） //B3LYP/6-31G（d,p） level for the DNA dinucleotide fragment, cy- tosine phosphate thymine （CpT）. Key factors blocking the formation of the SP-like product between two adjacent cytosine and thymidine residues are revealed. Instead of undergoing photochemical SP reaction, a photophysical deactivation pathway back to the ground state turns out to be favorable for the CpT dinucleotide fragment.展开更多
Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved t...Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved to be an excellent variety because of its strong resistance and high spore powder yield.Different cultivation materials were chosen and combined to form 3 cultivation formulations.The results showed that Formulation(3),in which basswood was soaked in nutrient solution for 24 h,presented fast mycelial growth and high spore powder output,and therefore was proved to be a high-yield formulation of Ganoderma lucidum spore powder.展开更多
Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC...Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC26854）, and an outstanding pathogenic activity against the green cabbage aphid, Brevicorine brassiacae （L.）. The isolates were grown on thirteen different liquid media, which had been used to produce other entomopathogenic Hyphomycetes. Experiments were carried out at 27 -4- 1 ℃ with a 12：12 photoperiod using shake flasks. The production of spores was quantified during a seven days period, and the effects of the media were evaluated by determining spore concentration and morphology. Submerged conidia yields were higher with ATCC26854 than with V3 in all thirteen media, while higher concentrations （5.3×10^9, 4.6 ×10^9 and 3.4 ×10^9 conidia/mL） were found with ATCC26854 isolate in the Camaron, Minerales and Jenkins-Prior medium, respectively; lower yields （2.3 ×10^8, 2.2 ×10^8 and 2.3 ×10^8 conidia/mL） were found with the V3 isolate in Catroux, TKI and Camaron media, respectively. Spore production curves were adjusted to different sigmoid models. The process was better explained by the Richards model （r^2 = 0.99）. Concerning conidia morphology, submerged conidia seemed to look like aerial conidia, but they were different in size （ATCC26854 2.73-6.99 μm and V3 5.28-14.29 μm）; however, the dimensions fall within the ranges reported for L. lecanii. The analysis of shake flask cultures with the Richards model allowed selecting two low-cost liquid medium, Camaron and Jenkins-Prior, for scaling up conidia production for use in aphid biological control programs.展开更多
The effect of the Entomopathogenic fungus, Paceilomyces farinosus on reproductive potential of female survivors, egg viability and total egg production of poplar leaf beetle Melasoma populi L. was investigated in Plan...The effect of the Entomopathogenic fungus, Paceilomyces farinosus on reproductive potential of female survivors, egg viability and total egg production of poplar leaf beetle Melasoma populi L. was investigated in Plant Protection Department laboratories, School of Plant Production, Faculty of Agriculture and Forestry, Duhok University in Spring 2011. Prepupae, pupae and newly emerged adults were treated with spore suspension of P.farinosus （1 ~ 10s conidia/mL）. Another group of adults were reared on sprayed poplar leaves with a same concentration of conidial suspension. Eggs production and hatching percentage were monitored daily over a 7-wk period. Overall reproductive capacity was significantly lower as compared with control. The total number of eggs per surviving female was 85.50, 149.50, 123.43 and 280.24 eggs for pre-pupa, pupa, adult and leaves treated, respectively as compared with 299.75 in control after fourth week. Pre-oviposition, ovipostion, post-oviposition days and the longevity of females and males were shorter in treated individuals compared with control. The lowest percentage hatching was 75.12% in eggs deposited by adults feed on treated leaves as compared with 98.42% in control.展开更多
The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal developme...The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.展开更多
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtrac...Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal de-velopment and pathogenicity in M. oryzae.展开更多
文摘The conidial tolerance of Metarhizium anisopliae var.anisopliae isolate ARSEF 2575 to UV-B irradiation is greatly influenced by growth-environment alterations.In this review,we report high variability in conidial UV-B tolerance in response to altered culture conditions.Conidia produced on insect cadavers[Zophobas morio(Coleoptera)or Galleria mellonella(Lepidoptera)] had low tolerance to UV-B radiation;and conidia produced on potato dextrose agar supplemented with yeast extract(PDAY)had medium UV-B tolerance;whereas conidia produced on a minimal medium without any carbon source(MM),on MM with a non-preferred carbon source such as lactose(=MML),on PDAY plus 1 M NaCl or KCl,or PDBY with high alkalinity had the highest UV-B tolerances.All of the above conditions that induced high UV-B tolerance,however,also greatly reduced conidial production.Comparisons between stress tolerance and conidial production,particularly with conidia produced under osmotic and nutritive stress,point out that the benefits of producing very tolerant conidia have the enormous cost of low conidial production.Growth under visible light also greatly improved conidial UV-B tolerance,but light did not negatively influence conidial production.Therefore,culture on rich media under light is proposed as the most promising approach to producing conidia with improved UV-B tolerance for biological control of pest insects in agriculture.
文摘As a well known DNA photolesion product, the special UV induced pyrimidine dimmer called spore photoproduct （SP）, has aroused strong research interests. The SP formation was reported solely between two adjacent thymidine residues. It remains unclear in pervious experimental observations why there is an absence of the cytosine-derived SP-like photoprod- uct formation at the cytosine containing DNA strand, although the cytosine residue holds great similarity to thymine in terms of molecular structure. From a theoretical perspec- tive, we have explored this issue in this work by means of density functional theory at the B3LYP/6-311＋＋G（d,p） //B3LYP/6-31G（d,p） level for the DNA dinucleotide fragment, cy- tosine phosphate thymine （CpT）. Key factors blocking the formation of the SP-like product between two adjacent cytosine and thymidine residues are revealed. Instead of undergoing photochemical SP reaction, a photophysical deactivation pathway back to the ground state turns out to be favorable for the CpT dinucleotide fragment.
文摘Comparative test of 4 Ganoderma lucidum varieties from different sources showed that the mycelium of Chizhi 1 grew fast with thick and dense hyphae,round and solid cap,and high spore powder yield.Chizhi 1 was proved to be an excellent variety because of its strong resistance and high spore powder yield.Different cultivation materials were chosen and combined to form 3 cultivation formulations.The results showed that Formulation(3),in which basswood was soaked in nutrient solution for 24 h,presented fast mycelial growth and high spore powder output,and therefore was proved to be a high-yield formulation of Ganoderma lucidum spore powder.
文摘Two Lecanicillium lecanii isolates, ATCC26854 and V3, were evaluated for spore production in liquid media. Both isolates have interesting known properties for the production of high concentrations of chitinases （ATCC26854）, and an outstanding pathogenic activity against the green cabbage aphid, Brevicorine brassiacae （L.）. The isolates were grown on thirteen different liquid media, which had been used to produce other entomopathogenic Hyphomycetes. Experiments were carried out at 27 -4- 1 ℃ with a 12：12 photoperiod using shake flasks. The production of spores was quantified during a seven days period, and the effects of the media were evaluated by determining spore concentration and morphology. Submerged conidia yields were higher with ATCC26854 than with V3 in all thirteen media, while higher concentrations （5.3×10^9, 4.6 ×10^9 and 3.4 ×10^9 conidia/mL） were found with ATCC26854 isolate in the Camaron, Minerales and Jenkins-Prior medium, respectively; lower yields （2.3 ×10^8, 2.2 ×10^8 and 2.3 ×10^8 conidia/mL） were found with the V3 isolate in Catroux, TKI and Camaron media, respectively. Spore production curves were adjusted to different sigmoid models. The process was better explained by the Richards model （r^2 = 0.99）. Concerning conidia morphology, submerged conidia seemed to look like aerial conidia, but they were different in size （ATCC26854 2.73-6.99 μm and V3 5.28-14.29 μm）; however, the dimensions fall within the ranges reported for L. lecanii. The analysis of shake flask cultures with the Richards model allowed selecting two low-cost liquid medium, Camaron and Jenkins-Prior, for scaling up conidia production for use in aphid biological control programs.
文摘The effect of the Entomopathogenic fungus, Paceilomyces farinosus on reproductive potential of female survivors, egg viability and total egg production of poplar leaf beetle Melasoma populi L. was investigated in Plant Protection Department laboratories, School of Plant Production, Faculty of Agriculture and Forestry, Duhok University in Spring 2011. Prepupae, pupae and newly emerged adults were treated with spore suspension of P.farinosus （1 ~ 10s conidia/mL）. Another group of adults were reared on sprayed poplar leaves with a same concentration of conidial suspension. Eggs production and hatching percentage were monitored daily over a 7-wk period. Overall reproductive capacity was significantly lower as compared with control. The total number of eggs per surviving female was 85.50, 149.50, 123.43 and 280.24 eggs for pre-pupa, pupa, adult and leaves treated, respectively as compared with 299.75 in control after fourth week. Pre-oviposition, ovipostion, post-oviposition days and the longevity of females and males were shorter in treated individuals compared with control. The lowest percentage hatching was 75.12% in eggs deposited by adults feed on treated leaves as compared with 98.42% in control.
基金Wenbing Yin is a scholar of "the 100 Talents Project" of Chinese Academy of Sciencessupported by the National Key Research and Development Program (2016YFD0400105)+1 种基金National Natural Science Foundation of China (31670402 and 31400334)Sate Key Laboratory of Mycology Open Project (SKLMKF 2015-1)
文摘The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.
基金Project supported by the National Natural Science Foundation of China (No. 30870101)the Public Welfare Profession (Agricul-ture) Research Project (No. 200803008), China
文摘Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal de-velopment and pathogenicity in M. oryzae.
