采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是...采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。展开更多
Hydrogen will be one of the most potential energy in the future mainly due to its cleanliness, renewablility and non-polluting nature.At present,the hydrogen-producing ability of bacteria is low, which is one of key f...Hydrogen will be one of the most potential energy in the future mainly due to its cleanliness, renewablility and non-polluting nature.At present,the hydrogen-producing ability of bacteria is low, which is one of key factors restricting the development of fermentative hydrogen production industry.Ultraviolet ray was used to mutate a wild hydrogen-producing strain,Ethanoligenens sp.ZGX4, in order to screen highly efficient H2-producing mutants.After investigating the hydrogen production stability of successive generations, a highly efficient hydrogen-producing mutant UV-d48 was obtained,and batch tests were conducted to investigate the H2-producing behavior of mutant UV-d48.The experiment results showed that UV-d48 had H2-producing capability of 2998.5 ml·L-1 and maximal H2 production rate of 34.4 mmol·(g drycell)-1·h-1 at 37℃,initial glucose concentration of 10 g·L-1 and pH of 6.0, which were higher by 65.1% and 56.4% than that of the wild parent strain ZGX4, respectively.Moreover, its hydrogen yield was estimated to be 2.61 mol H2·(mol glucose)-1, which was 1.54-fold higher than that of the control strain ZGX4.Since mutant UV-d48 performed typical ethanol-type fermentation in the course of H2 production, it might be used as a very important carrier material to investigate the metabolic pathway and mechanism of ethanol-type fermentation bacteria.展开更多
文摘采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。
文摘Hydrogen will be one of the most potential energy in the future mainly due to its cleanliness, renewablility and non-polluting nature.At present,the hydrogen-producing ability of bacteria is low, which is one of key factors restricting the development of fermentative hydrogen production industry.Ultraviolet ray was used to mutate a wild hydrogen-producing strain,Ethanoligenens sp.ZGX4, in order to screen highly efficient H2-producing mutants.After investigating the hydrogen production stability of successive generations, a highly efficient hydrogen-producing mutant UV-d48 was obtained,and batch tests were conducted to investigate the H2-producing behavior of mutant UV-d48.The experiment results showed that UV-d48 had H2-producing capability of 2998.5 ml·L-1 and maximal H2 production rate of 34.4 mmol·(g drycell)-1·h-1 at 37℃,initial glucose concentration of 10 g·L-1 and pH of 6.0, which were higher by 65.1% and 56.4% than that of the wild parent strain ZGX4, respectively.Moreover, its hydrogen yield was estimated to be 2.61 mol H2·(mol glucose)-1, which was 1.54-fold higher than that of the control strain ZGX4.Since mutant UV-d48 performed typical ethanol-type fermentation in the course of H2 production, it might be used as a very important carrier material to investigate the metabolic pathway and mechanism of ethanol-type fermentation bacteria.
