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平菇液体培养液全波长光谱特性与漆酶产量关系研究 被引量:5
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作者 程凡升 生吉萍 +1 位作者 王仁爱 申琳 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2009年第8期2157-2160,共4页
漆酶是一种广泛分布在真菌中的酶,其底物有非专一性,在木质素的降解和环境保护方面有重要作用。为测定和估计平菇菌丝液体发酵期间的漆酶产生量,使用紫外-可见分光光度计对菌液进行了全波长扫描,结合常规化学测定方法和平板固体培养基... 漆酶是一种广泛分布在真菌中的酶,其底物有非专一性,在木质素的降解和环境保护方面有重要作用。为测定和估计平菇菌丝液体发酵期间的漆酶产生量,使用紫外-可见分光光度计对菌液进行了全波长扫描,结合常规化学测定方法和平板固体培养基培养时的漆酶圈直径来进行研究,探明粗酶液全波长光谱特性与漆酶产生量的关系。结果发现,发酵粗酶液全波长扫描图谱在300nm处的波峰数目与漆酶的产量呈现相关关系;300nm处吸光值大于1.5的波长范围逐渐增大,11天的培养期间其波长范围从5nm变化到80nm与漆酶的产量呈正相关关系;在320~400nm出现有规律的光吸收,且在349,365,388nm处各出现光吸收峰,其中在365nm处出现最大吸收,且吸光值均与漆酶的产量呈正相关关系。据此可以应用于粗酶液中漆酶含量的估计或测定。与常规化学分析相比,全波长扫描方法有测定速度快、简单、节省溶剂、无化学污染等优点,为实际工业生产中的漆酶产量估计和测定提供了新方法。 展开更多
关键词 全波长扫描 光谱特性 漆酶 产生量估计
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Estimations of Net Primary Productivity and Evapotranspiration Based on HJ-1A/B Data in Jinggangshan City, China 被引量:4
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作者 ZHANG Rong-hua SUN Rui +5 位作者 DU Jun-ping ZHANG Ting-long TANG Yao XU Hong-wei YANG Sheng-tian JIANG Wei-guo 《Journal of Mountain Science》 SCIE CSCD 2013年第5期777-789,共13页
Net primary productivity (NPP) and evapotranspiration (ET) are two key variables in the carbon and water cycles of terrestrial ecosystems. In this study, to test a newly developed NPP algorithm designed for H J-1 ... Net primary productivity (NPP) and evapotranspiration (ET) are two key variables in the carbon and water cycles of terrestrial ecosystems. In this study, to test a newly developed NPP algorithm designed for H J-1 A/B data and to evaluate the usage of HJ-1 A/B data in the quantitative assessment of environments, NPP and ET in Jinggangshan city, Jiangxi province, are calculated using H J-1 A/B data. The results illustrate the following: (1) The NPP and ET in Jinggangshan city in 2olo both show obvious seasonal variation, with the highest values in summer and the lowest values in winter, and relatively higher values were observed in autumn than in spring. (2) The spatial pattern indicates that the annual NPP is high in the southern area in Jinggangshan city and low in the northern area. Additionally, high NPP is distributed in forests located in areas with high elevation, and low NPP is found in croplands at low elevations. ET has no significant north-south difference, with high values in the southeast and northwest and low values in the southwest, and high ET is distributed in forests at low elevations in contrast to low ET in forests in high-elevation areas and in cropland and shrub grassland in low-elevation areas. (3) Compared to the MODIS product, the range of H J-1 NPP is larger, and the spatial pattern is more coincident with the topography. The range of H J-1 ET is smaller than that of the MODIS product, and ET is underestimated to some extent but can reflect the effect of topography. This study suggests that the algorithm can be used to estimate NPP and ET in a subtropical monsoon climate if remotely sensed images with high spatial resolution are available. 展开更多
关键词 Net primary productivity REMOTESENSING EVAPOTRANSPIRATION HJ-~A/B data
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