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阿维链霉菌aveC基因缺失对产素调控的影响 被引量:1
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作者 彭敏 杨红文 +2 位作者 陈知龙 彭音 杨在清 《华中农业大学学报》 CAS CSCD 北大核心 2007年第1期63-66,共4页
通过构建仅含有aveC基因两端交换臂的缺失载体,与阿维链霉菌(Streptomyces avermitilis)染色体中aveC基因发生同源重组,以阻断该基因,从而获得aveC缺失突变株。并经摇瓶发酵和高效液相色谱检测不同阿维菌素组分产量。结果显示,aveC缺失... 通过构建仅含有aveC基因两端交换臂的缺失载体,与阿维链霉菌(Streptomyces avermitilis)染色体中aveC基因发生同源重组,以阻断该基因,从而获得aveC缺失突变株。并经摇瓶发酵和高效液相色谱检测不同阿维菌素组分产量。结果显示,aveC缺失突变株的阿维菌素的总产量与出发菌株的总产量差异不显著;而阿维菌素组分1的产量下降约58%,组分2的产量提高约52%。 展开更多
关键词 阿维菌 aveC 基因缺失 产素
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一株不吸水链霉菌的诱变育种及前体对产素的影响研究
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作者 王智学 冯健 +2 位作者 方新 于淼 蔡锐 《现代农业科技》 2008年第23期133-134,共2页
通过对一株不吸水链霉菌的诱变育种及前体对产素的影响研究,结果表明:LiCl对菌种诱变并不起作用,适当添加正丙醇能大幅度提高效价,Mn2+对产素没有影响,Cu2+对产素有很大影响,pH值下调数量对产素有明显影响。
关键词 不吸水链霉菌 诱变育种 前体 产素
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马哈利樱桃根际产吲哚乙酸细菌多样性及产素能力研究 被引量:5
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作者 赵柏霞 闫建芳 +2 位作者 夏国芳 李俞涛 潘凤荣 《江苏农业科学》 北大核心 2017年第20期169-172,共4页
分离6年生砂蜜豆/马哈利樱桃的根际细菌,通过16S rDNA基因序列分析确定分类地位,利用比色法筛选IAA产生菌并定量检测其产素能力。研究发现,共分离获得52株细菌,分属于2个类群11个属的17个种。对各代表菌株进行产素能力测定,共发现15株... 分离6年生砂蜜豆/马哈利樱桃的根际细菌,通过16S rDNA基因序列分析确定分类地位,利用比色法筛选IAA产生菌并定量检测其产素能力。研究发现,共分离获得52株细菌,分属于2个类群11个属的17个种。对各代表菌株进行产素能力测定,共发现15株菌可分泌生长素,其中A07、A20、B20及B26菌株具有较强的产素能力。马哈利砧木作为辽东地区甜樱桃的主要砧木,其根际促生菌的多样性分析及菌株利用研究较少,本研究为甜樱桃增产及生物菌肥开发提供了重要菌株资源。 展开更多
关键词 马哈利樱桃 根际细菌 多样性 吲哚乙酸 产素能力
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“鹤嘉”牌水稻增产素试验总结
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作者 刘龙 王豪利 《垦殖与稻作》 2002年第6期34-34,共1页
"鹤嘉"牌水稻增产素是鹤岗市鹤嘉腐殖酸有限公司生产的腐殖酸肥,属天然有机肥,是生产"绿色"食品的配套肥料.该肥黑色粉末状,易溶于水,肥效高,使用方法简单,省工、省力,现将使用效果总结如下:
关键词 鹤嘉牌水稻产素 腐殖酸肥 水温 杂草防除效果
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“农宝”丰产素真是农家之宝
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作者 郑际报 《技术开发与引进》 1997年第6期39-39,共1页
关键词 农宝丰产素 农作物 肥料
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筛选用于四川泡菜生物保鲜的产细菌素乳酸菌(英文) 被引量:9
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作者 饶瑜 王猛 +4 位作者 蒋云璐 常伟 陈功 向文良 车振明 《食品科学》 EI CAS CSCD 北大核心 2015年第3期171-177,共7页
从8 种中国传统四川泡菜中筛选产细菌素的乳酸菌,以用于四川泡菜的生物保鲜。从四川泡菜样品中共分离出227 株乳酸菌,并筛选出8 株能够产生细菌素类化合物抑制英诺克李斯特氏菌(Listeria innocua)、金黄色葡萄球菌(Staphylococcus aure... 从8 种中国传统四川泡菜中筛选产细菌素的乳酸菌,以用于四川泡菜的生物保鲜。从四川泡菜样品中共分离出227 株乳酸菌,并筛选出8 株能够产生细菌素类化合物抑制英诺克李斯特氏菌(Listeria innocua)、金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillus subtilis)和蜡样芽孢杆菌(Bacillus cereus)生长的乳酸菌。其中Lactobacillus harbinensis B22、屎肠球菌E6(Enterococcus faecium E6)和植物乳杆菌E11(Lb. plantarum E11)能够产生抑制革兰氏阴性菌大肠杆菌(Escherichia coli)生长的细菌素类化合物。进一步研究表明,这3 株乳酸菌耐酸、耐高盐,能够在四川泡菜模拟培养基中生长并产生耐酸耐高温的细菌素类化合物。结果表明,Lactobacillus harbinensis B22、屎肠球菌E6和植物乳杆菌E11可用于四川泡菜的生物保鲜。 展开更多
关键词 细菌乳酸菌 食品生物保鲜 筛选 四川泡菜 卷心菜汁培养基
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产细菌素乳酸菌在蔬菜发酵制品生物保鲜中的应用 被引量:10
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作者 饶瑜 常伟 +1 位作者 唐洁 李明元 《食品工业科技》 CAS CSCD 北大核心 2013年第16期392-395,400,共5页
生物保鲜(biopreservation)是当今食品保藏和食品安全研究的热点。近年来,大量的研究致力于细菌素和产细菌素菌种在食品生物保鲜中的应用策略。本文主要介绍最新的产细菌素乳酸菌(LAB)在蔬菜发酵制品中的应用,通过将产细菌素乳酸菌作为... 生物保鲜(biopreservation)是当今食品保藏和食品安全研究的热点。近年来,大量的研究致力于细菌素和产细菌素菌种在食品生物保鲜中的应用策略。本文主要介绍最新的产细菌素乳酸菌(LAB)在蔬菜发酵制品中的应用,通过将产细菌素乳酸菌作为发酵菌剂(starter culture)、保护菌剂(protective culture)或共培养菌剂(co-culture)运用到蔬菜的发酵制作中,以稳定蔬菜发酵过程中微生物体系,控制腐败和病原菌的存活和生长。 展开更多
关键词 细菌乳酸菌 蔬菜发酵 生物保鲜菌剂
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乳酸菌产细菌素的研究进展及其应用前景 被引量:27
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作者 陈静 张玉苍 何连芳 《安徽农业科学》 CAS 北大核心 2011年第4期1925-1927,共3页
细菌素是细菌产生的一种有抑制作用的杀菌蛋白或多肽物质。大部分细菌素只对有近缘关系的细菌有抑制作用,具有无毒、无副作用、无残留、无抗药性,对环境无污染等优点。笔者阐述了乳酸菌产细菌素近20年来的研究进展,对乳酸菌细菌素在工... 细菌素是细菌产生的一种有抑制作用的杀菌蛋白或多肽物质。大部分细菌素只对有近缘关系的细菌有抑制作用,具有无毒、无副作用、无残留、无抗药性,对环境无污染等优点。笔者阐述了乳酸菌产细菌素近20年来的研究进展,对乳酸菌细菌素在工业上的应用前景提出了展望。 展开更多
关键词 乳酸菌细菌 抑菌 研究进展 应用前景
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螺旋霉素高产株的推理选育和工业生产 被引量:6
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作者 宋友礼 屠小平 +1 位作者 胡巍 黄根娣 《中国医药工业杂志》 CAS CSCD 北大核心 2003年第11期552-554,共3页
产二素链霉菌 SIPI 90 0 4经自发或诱发的突变和推理选育 ,得到若干耐药性变种。第六代一株变种 10 - 2 9,产生螺旋霉素比原始菌株 SIPI90 0 4增产约 6倍。该变种应用于工业生产 (30 m3发酵罐 ) ,发酵效价、总单位和指数三项指标比原来... 产二素链霉菌 SIPI 90 0 4经自发或诱发的突变和推理选育 ,得到若干耐药性变种。第六代一株变种 10 - 2 9,产生螺旋霉素比原始菌株 SIPI90 0 4增产约 6倍。该变种应用于工业生产 (30 m3发酵罐 ) ,发酵效价、总单位和指数三项指标比原来生产菌株 F- 16分别提高 2 6 .1%、33.3%和 2 6 .8%。 展开更多
关键词 链霉菌 螺旋霉 推理育种 耐药性变种
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缩宫素在产程中的合理应用 被引量:1
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作者 王艳平 《现代保健(医学创新研究)》 2008年第12期155-156,共2页
自1954年,由du.vigneaud等合成缩宫素以来,已广泛应用于刺激子宫收缩、引产或促进分娩。合理应用缩宫素是计划分娩及催产成功的关键,如使用不当,可引起宫缩过强及胎儿窘迫,甚至子宫破裂、羊水栓塞等并发症,严重危及母儿生命。合... 自1954年,由du.vigneaud等合成缩宫素以来,已广泛应用于刺激子宫收缩、引产或促进分娩。合理应用缩宫素是计划分娩及催产成功的关键,如使用不当,可引起宫缩过强及胎儿窘迫,甚至子宫破裂、羊水栓塞等并发症,严重危及母儿生命。合理恰当使用缩宫素时产妇及胎儿起到积极有益作用,为减少产力异常所致难产发生率,降低手术产率及围产儿死亡率,故在临床中合理应用缩宫素应引起高度重视。 展开更多
关键词 产素 应用
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螺旋霉素高产菌种的推理选育 被引量:8
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作者 金志华 张定丰 +1 位作者 金一平 宋友礼 《中国抗生素杂志》 CAS CSCD 北大核心 1998年第2期133-135,共3页
根据螺旋霉素的生物合成途径对螺旋霉素产生菌产二素链霉菌进行推理选育,并获得了高产菌种。首先在紫外线诱变的基础上,筛选豆油耐性变株,得到菌株XC2-37,其发酵效价较出发菌株XC1-29提高9%。通过培养基优化,菌株X... 根据螺旋霉素的生物合成途径对螺旋霉素产生菌产二素链霉菌进行推理选育,并获得了高产菌种。首先在紫外线诱变的基础上,筛选豆油耐性变株,得到菌株XC2-37,其发酵效价较出发菌株XC1-29提高9%。通过培养基优化,菌株XC2-37的发酵效价提高61.8%。再以紫外线处理菌株XC2-37,并筛选缬氨酸诱导变株,得到菌株XC3-11,其发酵效价较菌株XC2-37提高20%。菌株XC3-11经自然分离得到菌株XC4-18,发酵效价达到4500u/ml,为原始出发菌株XC1-29的2.25倍。传代试验表明菌株XC4-18的高产遗传特性稳定。菌株XC4-18应用于50m3发酵罐进行工业化生产,与原始出发菌株XC1-29相比,发酵效价和发酵指数分别提高84.9%和43.6%。 展开更多
关键词 螺旋霉 链霉菌 菌种 推理选育
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关注天然产列腺素的益处
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《当代畜禽养殖业》 2002年第5期37-37,共1页
关键词 天然列腺 母猪 繁殖 性能
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寒冷凝集素对血细胞分析干扰的排除 被引量:10
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作者 程鲁京 薛华月 王卉 《武警医学院学报》 CAS 2000年第4期280-280,共1页
关键词 寒冷 血细胞分析仪 血常规
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元素星系化学演化的分析解
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作者 杜翠花 张波 《河北师范大学学报(自然科学版)》 CAS 2000年第4期465-468,共4页
利用瞬时循环近似 ,考虑元素产率随金属丰度的变化 ,得出了主要金属元素星系化学演化的分析解 .结合恒星核合成结果 ,计算了银河系中 O元素、Mg元素、Na元素、Sc元素等的丰度随金属丰度的变化规律 。
关键词 金属元 产素 银河系 金属丰度 星系化学演化 瞬时循环近似 分析解 核合成
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中药保健再生纤维素纤维的开发及应用 被引量:6
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作者 马君志 李昌垒 《针织工业》 2016年第8期4-6,共3页
为开发中药保健纤维素纤维,结合中药提取物技术,研究中药保健再生纤维素纤维的制备技术,介绍其工艺流程和生产工艺特点。重点对该类纤维的形态结构、物理机械性能、抗菌性能及纤维性能特点进行分析,同时,对中药保健再生纤维素纤维的开... 为开发中药保健纤维素纤维,结合中药提取物技术,研究中药保健再生纤维素纤维的制备技术,介绍其工艺流程和生产工艺特点。重点对该类纤维的形态结构、物理机械性能、抗菌性能及纤维性能特点进行分析,同时,对中药保健再生纤维素纤维的开发应用领域进行分析,并提出该类产品制备技术的两大发展趋势,包括微胶囊化技术和吸附材料微粒化技术,为中药保健再生纤维素纤维的开发和生产提供参考。 展开更多
关键词 中药保健再生纤维纤维 中药提取物 制备技术 抗菌性能 发展趋势
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Effect of N Fertilization on Yield, N Absorption and Utilization of Two Species of Super High-Yielding Summer Maize 被引量:6
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作者 王宜伦 刘天学 +2 位作者 谭金芳 张许 李潮海 《Agricultural Science & Technology》 CAS 2012年第2期339-342,374,共5页
[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilizati... [Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilization in accordance with different cultivars. [Method] Field experiment was conducted to study on effects of different N fertilizers on yield, N absorption and use efficiency of Zhengdan 958 and Xundan 20, in order to learn the effect differences at different N fertilizer levels. [Result] After N was applied, yields of the two summer maize increased significantly. Zhengdan 958 achieved the highest in yield and proceeds at 12 051.18 kg/hm2 and 1 722.40 yuan/hm2, respectively in low N level. In contrast, Xundan 20 achieved the highest at 13 166.00 kg/hm2 and 1 343.92 yuan/hm2 in the above two aspects in high N level. Compared with Zhengdan 958, Xundan 20 increased by 9.90%, 5.20% and 12.00% in N levels of 0, 240, and 450 kg/hm2, respectively. When N fertilizers were applied, protein yield of Xundan 20 was significantly higher than that of Zhengdan 958, so that higher N fertilizers contributed higher protein yield for Xundan 20. In high N level, N efficiency, N-fertilizer utilization and partial productivity of Xundan 20 were significantly higher than that of Zhengdan 958. [Conclusion] Lower N-fertilizer was suitable for Zhengdan 958 and Xundan 20 would get a good harvest if more N-fertilizers were applied. The results provided references for reasonable N fertilization. 展开更多
关键词 Super high-yielding Summer maize CULTIVAR N-FERTILIZER YIELD Accumulation of N N efficiency
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The Principal Component Analysis on Yielding and Agronomic Traits of Hybrid Rice of Liangyou 2111 被引量:5
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作者 吕宏斌 钱敏 +9 位作者 李朝华 徐加万 丁明亮 刘宏珺 梅新彪 王海德 陈良 黄洁 杨林仙 李政芳 《Agricultural Science & Technology》 CAS 2017年第3期483-486,共4页
In order to define the relationship between yield and important agronomic traits of two lines hybrid Uangyou 2111, the principal component analysis method was used to analyze the expadmental data of six test points in... In order to define the relationship between yield and important agronomic traits of two lines hybrid Uangyou 2111, the principal component analysis method was used to analyze the expadmental data of six test points in Yunnan Province. The results showed that the main factors influencing the production of Liangyou 2111 were grain number, grains seed number, panicle length, growth padod and panicle rate; then were 1 O00-grain weight, seed setting rate, effective panicle and highest stem tillers number; again was plant height. Therefore, when hybrid rice of Uangyou 2111 will be planted widely in yunnan province, we should focus on en- sudng the panicle traits, especially increase grain number and grain seed number, and coordinately develop other traits to achieve high yield. 展开更多
关键词 RICE Two "lines hybrid of Liangyou 2111 Yielding traits Principal com-ponent analysis
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Effects of Green Manure Rotation on Rice Growth Dynamics and Nitrogen Uptake and Utilization 被引量:3
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作者 张立进 杨滨娟 +2 位作者 黄国勤 陈洪俊 刘康 《Agricultural Science & Technology》 CAS 2015年第5期962-967,共6页
This study aimed to comprehensive evaluation of different winter green manure on characterization of nitrogen uptake and utilization, to provide the basis for N fertilizer reasonable operation and characteristics impr... This study aimed to comprehensive evaluation of different winter green manure on characterization of nitrogen uptake and utilization, to provide the basis for N fertilizer reasonable operation and characteristics improvement of nitrogen nutrition in rice high-yield cultivation. This experiment was set to compare milk vetch, rapeseed,ryegrass and mixed of green manure on rice yield, rice growth dynamics and nitrogen uptake and utilization in rice. The results showed that among 4 different winter green manure, the treatment of MV-R-R(milk vetch-rice-rice) for the early rice yield was the most than others treatments. Compared with RG-R-R(ryegrass-rice-rice), RPR-R(rapeseed-rice-rice), MS-R-R(mixed green manure-rice-rice), the treatment of MVR-R inceased by 6.61%, 3.29%, 0.78%, respectively. The treatment of MV-R-R in N content in plant of rice was maximized in the tillering, booting, heading and maturity periods, respectively higher than the average of other treatments 9.68%, 19.72%,6.23% and 8.66%. At tillering, booting, heading and maturity, the treatment of MV-R-R were the highest in N uptake, RP-R-R minimum. The N periodic accumulation for MV-R-R were higher than other treatments in the tillering to booting, booting to heading and heading to maturity periods. The rates respectively were 21.81%, 68.73% and286.5%. In addition, N periodic accumulation and its ratio to total in the heading to maturity was minimum, maximum before tillering under green manure rotation system.So the cropping system of milk vetch-rice-rice could increase nitrogen use efficiency and improve N cycling. 展开更多
关键词 Winter green manure Rice yield Nitrogen uptake and utilization Paddy field
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Effect of Transplanting Density on Rice Yield,Nitrogen Uptake and ^(15)N-fertilizer Fate 被引量:5
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作者 樊红柱 张鸿 +2 位作者 冯文强 张冀 王昌桃 《Agricultural Science & Technology》 CAS 2012年第5期1037-1039,1054,共4页
[Objective] The aim of this study was investigated the rice yield, nitrogen uptake and ^15-fertilizer fate at different transplanting density to provide scientific ba- sis for improving the yield of rice and applying ... [Objective] The aim of this study was investigated the rice yield, nitrogen uptake and ^15-fertilizer fate at different transplanting density to provide scientific ba- sis for improving the yield of rice and applying reasonably fertilizer. [Method] A field experiment was carried out to study the effect of different transplanting density on rice yield, nitrogen (N) absorption, sources of N uptake by rice and the N balance in the plant-soil systems by using ^15-labelled urea. [Result] There were no significant differences in rice yields and total N uptakes by rice between treatments 30 cm × 30 cm and 40 cm × 40 cm, but the yield of rice and total N absorption in the two treatments were remarkably higher than those in 50 cm × 50 cm treatment. The amounts of total N uptake by rice were in the range of 112.3-162.7 kg/hm2 in the three transplanting densities. The result showed that about 1/3 of the total N uptake by rice was supplied by application fertilizer and the other 2/3 was obtained from the soil N pool. The ^15N-labelled urea absorbed by rice, residual in soil and lost accounted for 16.3%-26.1%, 17.0%-20.9% and 53.0%-66.7% of the total fertilizer, respectively. A great deal of ^15N-labelled urea was lost during the rice growing season. [Conclusion] Considering the rice yield and environmental protection, the transplanting density of 30 cm×30 cm was recommended in the hilly area of Sichuan basin in the southwest China. 展开更多
关键词 Rice yield Nitrogen uptake and balance ^15N-labeled urea ^15N fate Transplanting density
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Distribution of Oxytocin Hypothamic-Picuitary-Ovary Axis in Guangxi Local Buffalo 被引量:6
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作者 凌泽继 韩涛 +7 位作者 李瑞明 秦津 谢莹雪 许典新 杨柄壮 潘琼 奉倩 宋小白 《Agricultural Science & Technology》 CAS 2010年第5期138-143,154,共7页
To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochem... To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuperPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo. The test results could provide morphology according to study the OT's synthesis and mechanism of action,and could play reference and directions part in breeding Guangxi local buffalo. The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventromedial nucleus,ventrolateralis nucleus,suprachiasmaticus nucleus,dorsomedial nucleus,mamillary body,anterior hypothalamic nucleus and so on. The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence. In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein cells. The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo. The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus. 展开更多
关键词 OT Guangxi local buffalo Hypothalamuses-pituitary-ovary axis Immunohistochemistry SuperPicTureTM two step method
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