河道疏浚会产生大量疏浚底泥,其水分含量很高,不利于处理和二次利用。而将微生物絮凝剂用于河道疏浚底泥快速脱水,具有高效、无毒、无二次污染的特点。对菌株LMB8进行产絮凝剂优化培养后,将其所产微生物絮凝剂用于河道疏浚底泥快速脱水...河道疏浚会产生大量疏浚底泥,其水分含量很高,不利于处理和二次利用。而将微生物絮凝剂用于河道疏浚底泥快速脱水,具有高效、无毒、无二次污染的特点。对菌株LMB8进行产絮凝剂优化培养后,将其所产微生物絮凝剂用于河道疏浚底泥快速脱水。实验结果表明,当底泥泥浆(含水率为93%)体积为100 m L,p H值为9,助凝剂Ca Cl2溶液投加量为5 m L,LMB8菌株发酵液的最适投加量为3 m L时,LMB8菌株产絮凝剂对河道疏浚底泥泥浆的絮凝率最高可达83.1%。LMB8菌株所产絮凝性物质中含有多糖,不含有蛋白质,具有热稳定性。LMB8菌体本身则不具有絮凝性,其有效絮凝性物质是该菌体在发酵过程中产生的胞外分泌物。展开更多
[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on t...[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.展开更多
The flucculation efficiency of compound bioflocculant produced by flocculant-producing bacteria was investigated in this study. Cheap cellulose was selected as the substrate for the production of a lower cost bioflocc...The flucculation efficiency of compound bioflocculant produced by flocculant-producing bacteria was investigated in this study. Cheap cellulose was selected as the substrate for the production of a lower cost bioflocculant. The end product of cellulose decomposing bacteria was utilized as substrate for flocculant-producing bacteria. The optimum fermentation conditions were determined as follows： the initial fermentation and fermentation time was 5 d and 1 d respectively, the temperature was 30 ℃, the rotation speed was 120 r/min, the amount of CaCl2 solution （ 10% ） was 1.5 ml/L. The flocculation test indicated that the bioflocculant had high efficiency in the removal of the turbldity raw water from Songhua River.展开更多
文摘河道疏浚会产生大量疏浚底泥,其水分含量很高,不利于处理和二次利用。而将微生物絮凝剂用于河道疏浚底泥快速脱水,具有高效、无毒、无二次污染的特点。对菌株LMB8进行产絮凝剂优化培养后,将其所产微生物絮凝剂用于河道疏浚底泥快速脱水。实验结果表明,当底泥泥浆(含水率为93%)体积为100 m L,p H值为9,助凝剂Ca Cl2溶液投加量为5 m L,LMB8菌株发酵液的最适投加量为3 m L时,LMB8菌株产絮凝剂对河道疏浚底泥泥浆的絮凝率最高可达83.1%。LMB8菌株所产絮凝性物质中含有多糖,不含有蛋白质,具有热稳定性。LMB8菌体本身则不具有絮凝性,其有效絮凝性物质是该菌体在发酵过程中产生的胞外分泌物。
基金Supported by the Science Research Project of Qingdao Technical College in 2012(12-A-2)~~
文摘[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.
基金Sponsored by the Science Foundation of Heilongjiang Province(Grant No.GB02C202 -02).
文摘The flucculation efficiency of compound bioflocculant produced by flocculant-producing bacteria was investigated in this study. Cheap cellulose was selected as the substrate for the production of a lower cost bioflocculant. The end product of cellulose decomposing bacteria was utilized as substrate for flocculant-producing bacteria. The optimum fermentation conditions were determined as follows： the initial fermentation and fermentation time was 5 d and 1 d respectively, the temperature was 30 ℃, the rotation speed was 120 r/min, the amount of CaCl2 solution （ 10% ） was 1.5 ml/L. The flocculation test indicated that the bioflocculant had high efficiency in the removal of the turbldity raw water from Songhua River.
