微藻作为21世纪生物柴油的理想燃料已被人们广泛的关注,但是目前微藻种类很多,如何从诸多的微藻中筛选油脂含量高的微藻已成为人们函待解决的问题。从东北地区水样中分离纯化出93种藻种,采用尼罗红染色法对其中30株藻种进行了筛选获得了...微藻作为21世纪生物柴油的理想燃料已被人们广泛的关注,但是目前微藻种类很多,如何从诸多的微藻中筛选油脂含量高的微藻已成为人们函待解决的问题。从东北地区水样中分离纯化出93种藻种,采用尼罗红染色法对其中30株藻种进行了筛选获得了8种具有产油潜力的藻种,并利用自制的柱式反应器微藻评价装置对这8株藻株进行了产油能力的评价,获得了一株总脂产率达到133.9 mg/(L·d)的产油能源微藻。在此基础上,对该藻株进行了18S r RNA的鉴定,确定为Chlorella sp.。展开更多
Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to ＂green diesel＂. Increasing mi...Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to ＂green diesel＂. Increasing microalgae lipids production by thermal stressing is important in improving the economics of biodiesel production, but its effectiveness needs to be determined. This paper focuses on the effect of cooling stressing lipid triggering on the microalgae production and lipids yield. Two microalgae species were studied, Chlorella sp. and Dunaliella. In each case, microalgae were grown in two identical 2 L PBRs （photobiorectors） at room temperature. At the end of the exponential growth phase, one PBR was placed in a cold environment while the other PBR was left at room temperature. Microalgae was harvested, freeze dried and the algae oil was extracted. Measurements show that cooling stressing slightly increased the biomass of algae （11% for C2 and 13% for Dunaliella）, but it decreased the lipids content of the microalgae, 62% for Chlorella sp. and 13% for Dunaliella. The net effect is a decrease in the lipid production rate （mg lipid/L-day） 58% in case of Chlorella sp., and 2% in case of Dunaliella.展开更多
To study the abilities of Chlorella sorokiniana CS-01 on using CO2 from flue gases to produce biodiesel,the microaglae was cultured with different simulated flue gases containing 5%-15%(volume fraction) of CO2.The res...To study the abilities of Chlorella sorokiniana CS-01 on using CO2 from flue gases to produce biodiesel,the microaglae was cultured with different simulated flue gases containing 5%-15%(volume fraction) of CO2.The results show that strain CS-01 could grow at 15% CO2 and grow well under CO2 contents ranging from 5%-10%.The maximal biomass productivity and lipid productivity were obtained when aerating with 10% of CO2.The lipids content ranged from 28% to 43% of dry mass of biomass.The main fatty acid compositions of strain CS-01 were C14-C18(>72%) short-chain FAMEs(known as biodiesel feedstocks).Meanwhile,the biodiesel productivity was over 60%,suggesting that Chlorella sorokiniana CS-01 has a great potential for CO2 mitigation and biodiesel production.Furthermore,differential expression of three genes related to CO2 fixation and fatty acid synthesis were studied to further describe the effect of simulated flue gases on the growth and lipid accumulation of strain CS-01 at molecular level.展开更多
文摘微藻作为21世纪生物柴油的理想燃料已被人们广泛的关注,但是目前微藻种类很多,如何从诸多的微藻中筛选油脂含量高的微藻已成为人们函待解决的问题。从东北地区水样中分离纯化出93种藻种,采用尼罗红染色法对其中30株藻种进行了筛选获得了8种具有产油潜力的藻种,并利用自制的柱式反应器微藻评价装置对这8株藻株进行了产油能力的评价,获得了一株总脂产率达到133.9 mg/(L·d)的产油能源微藻。在此基础上,对该藻株进行了18S r RNA的鉴定,确定为Chlorella sp.。
文摘Microalgae lipids/oils are a promising feedstock for biodiesel production. The desired lipids are triacylglycerols. These can either be transesterified to biodiesel or decarboxylated to ＂green diesel＂. Increasing microalgae lipids production by thermal stressing is important in improving the economics of biodiesel production, but its effectiveness needs to be determined. This paper focuses on the effect of cooling stressing lipid triggering on the microalgae production and lipids yield. Two microalgae species were studied, Chlorella sp. and Dunaliella. In each case, microalgae were grown in two identical 2 L PBRs （photobiorectors） at room temperature. At the end of the exponential growth phase, one PBR was placed in a cold environment while the other PBR was left at room temperature. Microalgae was harvested, freeze dried and the algae oil was extracted. Measurements show that cooling stressing slightly increased the biomass of algae （11% for C2 and 13% for Dunaliella）, but it decreased the lipids content of the microalgae, 62% for Chlorella sp. and 13% for Dunaliella. The net effect is a decrease in the lipid production rate （mg lipid/L-day） 58% in case of Chlorella sp., and 2% in case of Dunaliella.
基金Project(50621063) supported by the National Natural Science Foundation for Distinguished Group of ChinaProjects(2010bsxt05,2010ssxt246) supported by the Innovation Foundation of Science and Technology of Central South University,China
文摘To study the abilities of Chlorella sorokiniana CS-01 on using CO2 from flue gases to produce biodiesel,the microaglae was cultured with different simulated flue gases containing 5%-15%(volume fraction) of CO2.The results show that strain CS-01 could grow at 15% CO2 and grow well under CO2 contents ranging from 5%-10%.The maximal biomass productivity and lipid productivity were obtained when aerating with 10% of CO2.The lipids content ranged from 28% to 43% of dry mass of biomass.The main fatty acid compositions of strain CS-01 were C14-C18(>72%) short-chain FAMEs(known as biodiesel feedstocks).Meanwhile,the biodiesel productivity was over 60%,suggesting that Chlorella sorokiniana CS-01 has a great potential for CO2 mitigation and biodiesel production.Furthermore,differential expression of three genes related to CO2 fixation and fatty acid synthesis were studied to further describe the effect of simulated flue gases on the growth and lipid accumulation of strain CS-01 at molecular level.
