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一种提高古龙酸大菌的产酸方法
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作者 秦建萍 《农家科技(理论版)》 2019年第2期60-60,共1页
目的:利用紫外线改变DNA 的分子结构的方法对生产古龙酸的大菌进行诱变选育来获得高产菌株。方法:以大菌CX75001为出发菌株通过紫外诱变的方法改变生产菌株的遗传性特征以此来提高古龙酸代谢山梨糖的能力使其酸值产量提高,并利用96孔板... 目的:利用紫外线改变DNA 的分子结构的方法对生产古龙酸的大菌进行诱变选育来获得高产菌株。方法:以大菌CX75001为出发菌株通过紫外诱变的方法改变生产菌株的遗传性特征以此来提高古龙酸代谢山梨糖的能力使其酸值产量提高,并利用96孔板发酵结合快速酸值测定进行高通量筛选。结果:诱变时间60s时诱变菌株产酸量有较大幅度提高。通过三轮复筛验证最终获得7 株产量分别比出发菌株提高了47.78%,46.67%,38.89%,45.56%,48.89%,43.33%,42.22%的突变菌株。结论:利用紫外诱变可以提高大菌的产酸量。 展开更多
关键词 紫外诱变 产酸增强 遗传性改变 高通量筛选
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Maillard Products Formed from Chicken Fat Oxidants and Amino Acids Act as a Flavor Enhancer in Comminuted Chicken Products 被引量:2
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作者 Lingling Liu Boyan Liu +2 位作者 Yahui Cui Yanwen Wu Jie Ouyang 《Journal of Food Science and Engineering》 2013年第3期161-167,共7页
Lipid oxidation and degradation are generally recognized as unfavorable reactions in food processing. However, lipids can be utilized to produce meat-like flavorings through reaction with amino acids after degradation... Lipid oxidation and degradation are generally recognized as unfavorable reactions in food processing. However, lipids can be utilized to produce meat-like flavorings through reaction with amino acids after degradation to carbonyls such as aldehydes and ketones. The fatty acids in chicken fat mainly comprise palmitic acid, oleic acid and linoleic acid. After being heated at 120 ~C for 3 h, the degraded carbonyls of chicken fat were reacted with cysteine, alanine, glycine and thiamine to form a chicken-like flavoring. The key aromatic components of the flavoring were identified by GC-MS. In the overall volatile profile, 22 kinds of compound were detected, among which 2-furanmethanol was formed by the Maillard reaction, 1-(2-furanyl)-ethanone by cyclization and 2,4,6-trimethyl-l,3,5-trithiane by the Strecker degradation of cysteine. Dihydro-2-methyl-3(2H)-furanone, dihydro-2-methyl- 3(2H)-thiophenone, 2-acetyl-lH-pyrroline and 4-methyl-5-thiazoleethanol were the degradation products of thiamine. The prepared chicken flavoring was added to comminuted chicken product (CCP). GC-MS analysis and sensory evaluation showed that the flavor and odor of the CCP were greatly enhanced. 展开更多
关键词 Chicken fat lipid oxidation Maillard reaction flavor enhancer comminuted chicken product
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