28 oilseed rape Pol CMS three-line hybrid combinations diallel-crossed were compared in the yield and harvest index and analyzed on the correlation between the experimental yields and harvest indexes in this study. Th...28 oilseed rape Pol CMS three-line hybrid combinations diallel-crossed were compared in the yield and harvest index and analyzed on the correlation between the experimental yields and harvest indexes in this study. The correlation coeffcient was only 0.365 4, lower than a signifcant level, which indicated that there was no signifcant positive （or negative） correlation between the economic yields and the harvest indexes in oilseed rape. Among them, 8 hybrid combinations including 7 with a harvest index〉0.30 and one with a harvest index〈0.27 increased signifcantly in the yields compared with the control, and then were screened for production experiment. Under different cultivation methods, all the 8 combinations had a stable harvest index, and the combinations with higher harvest indexes also had a stable performance in yields. An oilseed rape variety Fengyou 737 with higher yield and harvest index selected through a further screening was grown with the harvest indexhigher than 0.33 whether transplanted or directly seeded in Yangtze River Basin Demonstration Area, demonstrating stable high yields as well as good ecological adaptability. The combination of yield and harvest index in the study is conducive to breeding a new oilseed rape variety with stable yields and good tolerance to close planting.展开更多
The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulf...The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate（CuSO4）.The best conditions obtained from screening experiments in shaken flasks using tomato juice,CuSO4,and soybean oil were integrated in an experimental design.Enhanced levels of tomato juice as the medium,CuSO4and soybean oil as inducers（36.8%（v/v）,3 mmol/L,and 1%（v/v）,respectively） were determined for 10 L stirred tank bioreactor runs.This combination resulted in laccase titer of 143000 IU/L（2,2＇-azino-bis（3-ethylbenzthiazoline-6- sulfonic acid）,pH 3.0）,which represents the highest activity so far reported for P.sanguineus in a 10-L fermentor.Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20000 IU/L,whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times.Based on a partial characterization,the laccases of this strain are especially promising in terms of thermostability（half-life of 6.1 h at 60 °C） and activity titers.展开更多
文摘28 oilseed rape Pol CMS three-line hybrid combinations diallel-crossed were compared in the yield and harvest index and analyzed on the correlation between the experimental yields and harvest indexes in this study. The correlation coeffcient was only 0.365 4, lower than a signifcant level, which indicated that there was no signifcant positive （or negative） correlation between the economic yields and the harvest indexes in oilseed rape. Among them, 8 hybrid combinations including 7 with a harvest index〉0.30 and one with a harvest index〈0.27 increased signifcantly in the yields compared with the control, and then were screened for production experiment. Under different cultivation methods, all the 8 combinations had a stable harvest index, and the combinations with higher harvest indexes also had a stable performance in yields. An oilseed rape variety Fengyou 737 with higher yield and harvest index selected through a further screening was grown with the harvest indexhigher than 0.33 whether transplanted or directly seeded in Yangtze River Basin Demonstration Area, demonstrating stable high yields as well as good ecological adaptability. The combination of yield and harvest index in the study is conducive to breeding a new oilseed rape variety with stable yields and good tolerance to close planting.
基金supported by the National Science and Technology Council of Mexico(CONACYT)(No.192118)
文摘The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate（CuSO4）.The best conditions obtained from screening experiments in shaken flasks using tomato juice,CuSO4,and soybean oil were integrated in an experimental design.Enhanced levels of tomato juice as the medium,CuSO4and soybean oil as inducers（36.8%（v/v）,3 mmol/L,and 1%（v/v）,respectively） were determined for 10 L stirred tank bioreactor runs.This combination resulted in laccase titer of 143000 IU/L（2,2＇-azino-bis（3-ethylbenzthiazoline-6- sulfonic acid）,pH 3.0）,which represents the highest activity so far reported for P.sanguineus in a 10-L fermentor.Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20000 IU/L,whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times.Based on a partial characterization,the laccases of this strain are especially promising in terms of thermostability（half-life of 6.1 h at 60 °C） and activity titers.
