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AFLP Analysis of Phylogenetic Relationship of Salvia spp. in Beijing 被引量:1
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作者 杨建玉 陈洪伟 +4 位作者 刘克锋 王红利 刘永光 王顺利 金珠理达 《Agricultural Science & Technology》 CAS 2010年第2期72-75,共4页
Twenty-third collections of Salvia spp.in Beijing were analyzed phylogenetically by AFLP technique.6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DN... Twenty-third collections of Salvia spp.in Beijing were analyzed phylogenetically by AFLP technique.6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DNA.All of 367 AFLP bands were obtained,in which 359(97.82%)were polymorphic markers.At a genetic distance of 0.32,the collections could be clustered into 7 species i.e.S.splendens,S.farinacea,S.coccinea,S.plebeia,S.miltiorrhiza,S.umbratica and S.officinalis.The respective genetic relationship be... 展开更多
关键词 Salvia spp. AFLP Genetics analysis Salvia splendens
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向群众讲政策
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作者 高有才 李丽琼 《新湘评论》 1995年第3期34-34,共1页
关键词 中国农民 棉花收购 讲政策 棉价 管理不善 头文件 卷过 质损 亲串 膝盖
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Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry 被引量:4
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作者 郭萌萌 吴海燕 +5 位作者 江涛 谭志军 赵春霞 郑关超 李兆新 翟毓秀 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2017年第4期883-893,共11页
In this study, we established a comprehensive method for simultaneous identification and quantification of tetrodotoxin (TTX) in fresh pufferfish tissues and pufferfish-based products using liquid chromatography/qua... In this study, we established a comprehensive method for simultaneous identification and quantification of tetrodotoxin (TTX) in fresh pufferfish tissues and pufferfish-based products using liquid chromatography/quadrupole-linear ion trap mass spectrometry (LC-QqLIT-MS). TTX was extracted by 1% acetic acid-methanol, and most of the lipids were then removed by freezing lipid precipitation, followed by purification and concentration using immunoaffinity columns (IACs). Matrix effects were substantially reduced due to the high specificity of the IACs, and thus, background interference was avoided. Quantitation analysis was therefore performed using an external calibration curve with standards prepared in mobile phase. The method was evaluated by fortifying samples at 1, 10, and 100 ng/g, respectively, and the recoveries ranged from 75.8%--107%, with a relative standard deviation of less than 15%. The TTX calibration curves were linear over the range of 1-1 000 ~tg/L, with a detection limit of 0.3 ng/g and a quantification limit of 1 ng/g. Using this method, samples can be further analyzed using an information- dependent acquisition (IDA) experiment, in the positive mode, from a single liquid chromatography-tandem mass spectrometry injection, which can provide an extra level of confirmation by matching the full product ion spectra acquired for a standard sample with those from an enhanced product ion (EPI) library. The scheduled multiple reaction monitoring method enabled TTX to be screened for, and TTX was positively identified using the IDA and EPI spectra. This method was successfully applied to analyze a total of 206 samples of fresh pufferfish tissues and pufferfish-based products. The results from this study show that the proposed method can be used to quantify and identify TTX in a single run with excellent sensitivity and reproducibility, and is suitable for the analysis of complex matrix pufferfish samples. 展开更多
关键词 TETRODOTOXIN fresh pufferfish pufferfish-based product immunoaffinity column liquidchromatography/quadrupole-linear ion trap mass spectrometry
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论傣族乐器“光邦”在节庆仪式场域中的文化意义
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作者 金红 《西南边疆民族研究》 CSSCI 2015年第2期130-136,共7页
民族民间艺术多是由仪式和仪式中的艺术经一系列过程性行为构成相应的文化事项。云南省德宏傣族景颇族自治州盈江县支那乡支那村的傣族膜鸣乐器"光邦",是典型的依托宗教仪式和民俗仪式鲜活地传承发展于当下的民族民间音乐艺术... 民族民间艺术多是由仪式和仪式中的艺术经一系列过程性行为构成相应的文化事项。云南省德宏傣族景颇族自治州盈江县支那乡支那村的傣族膜鸣乐器"光邦",是典型的依托宗教仪式和民俗仪式鲜活地传承发展于当下的民族民间音乐艺术,具有丰富的综合文化意义。本文运用音乐社会学的理论与方法,以春节期间使用乐器"光邦"进行一系列拜年仪式活动过程为考察对象,从村民精神诉求、村落主导价值、汉傣文化融合、传统教育观念几方面解读该仪式的文化意义。 展开更多
关键词 傣族乐器 光邦 亲串寨拜年仪式 文化意义
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晚年夸儿媳
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作者 安保障 《山西老年》 2004年第6期26-27,共2页
关键词 亲串 安保 自料 毛巾被 大房间 议订 汗衫 小房间 凉鞋 睡床
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A facile synthesis of 2-aryloxypyrimidine derivatives via a tandem reductive amination/intermolecular S_NAr sequence 被引量:4
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作者 Hai-feng WU Pei-zhi ZHANG Jun WU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第2期94-101,共8页
A novel tandem reductive amination/intermolecular nucleophilic aromatic substitution (SNAr) sequence has been established for the synthesis of amine containing pyrimidine in formation of one carbon-oxygen and one carb... A novel tandem reductive amination/intermolecular nucleophilic aromatic substitution (SNAr) sequence has been established for the synthesis of amine containing pyrimidine in formation of one carbon-oxygen and one carbon-nitrogen bonds in a one-pot fashion. Treatment of aldehyde with arylamine, 2-methanesulfonyl-4,6-dimeth-oxypyrimidine and sodium borohydride provides good overall yield. The p-toluenesulfonic acid (PTSA) can be used as activator and is generally needed in the reaction. Dioxane is the preferred reaction solvent, but reactions can also be carried out in tetrahydrofuran (THF), MeCN, toluene and dichloromethane. The procedure is carried out effectively in the presence of K2CO3. The reaction proceeds smoothly with aromatic aldehydes and arylamines possessing elec-tron-donating or-withdrawing groups. This method can be applied to the synthesis of the oilseed rape herbicide and is superior to the classical one in several aspects: cutting out several purification steps, minimizing solvent use and chemical waste, and saving time. Its advantages such as operational convenience, high-efficient synthesis, and starting material availability make it a desirable method for preparing amines with molecular diversity and biological activity. 展开更多
关键词 Reductive amination/intermolecular SNAr C-O and C-N bonds Amine PYRIMIDINE HERBICIDE
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