Orlicz准范‖·‖_ρ与其亲模ρ的关系及其反演问题

讨论了线性空间X上的模数ρ与其生成的Orlicz准范‖·‖，之间的关系，给出了六个关系命题；提出了准范的反演问题，得到了两个重要结果：线性空间上的任何β-范数（0＜β＜1）都是可反演的，而任何范数（β＝1）都是不可反演的．

Enantioselective extraction of clorprenaline enantiomers with hydrophilic selector of sulfobutylether-β-cyclodextrin by experiment and modeling

Enantioselective extraction of hydrophobic clorprenaline （CPE） enantiomers from organic phase to aqueous phases with sulfobutylether-β-cyclodextrin （SBE-β-CD） as the selector was investigated with insight into a number of important process variables, such as the type of organic solvent, concentration of selector, pH, and temperature. Equilibrium of the extraction system was modeled using a reactive extraction modcl with a homogeneous aqueous phase reaction. The important parameters of this model were determined experimentally. The physical distribution coefficients for molecular and ionic CPE were determined as 0.3 and 8.93, respectively. The equilibrium constants of the complexation reaction with SBE-β-CD were determined as 152 and 110 L/mol for R- and S-CPE, respectively. Results show that the experimental data agree with the model predictions perfectly. Comprehensively considering the experiment and model, the extraction conditions are optimized and the best extraction conditions are： pH of 6.0, SBE-β-CD concentration of 0.04 tool/L, and temperature of 5 ℃, providing the enantioselectivity （a） of 1.25, the fraction of R-CPE （φR） in aqueous phase of 0.71 and performance factor （pf） of 0.025.

Acid Dissociation Constants of Functional Groups in Humic Substances:I.Affinity Spectrum Model Analysis of Potentiometric Data of Fulvic and Humic Acids Solutions from Weathered Coal and Dark Loessial Soil

Potentiometric experiments were carried out on the proton binding equilibria of FA extracted from a weathered coal and HA and Fa extracted from a dark loessial soil.The affinity spectrum model was employed to treat the experimental data.The affinity spectrum model technique could“magnify” the heterogeneity of the proton binding equilibria.so it was useful for comparing and studying the characteristics of humic substances with similar properties.According to the affinity spectra,we also found that the direction of the titration could affect the properties of the equilibria of FA from the weathered coal,and the acidic functional groups contained in FA from the weathered coal were larger in quantity than those contained in HA and FA from the dark loessial soil.

Inheritance Pattern of Microsatellite Loci and Their Use for Kinship Analysis in the Japanese Scallop Patinopecten yessoensis

The inheritance mode of seven microsatellite markers was investigated in Patinopecten yessoensis larvae from four con-trolled crosses,and the feasibility of using these markers for kinship estimation was also examined. All the seven microsatellite loci were compatible with Mendelian inheritance. Neither sex-linked barriers to transmission nor major barriers to fertilization between gametes from the parents were evident. Two of the seven loci showed the presence of null alleles in two families,suggesting the need to conduct comprehensive species-specific inheritance studies for microsatellite loci used in population genetic studies. However,even if the null allele heterozygotes were considered as homozygotes in the calculation of genetic distance,offspring from four families were all unambiguously discriminated in the neighbor-joining dendrogram. This result indicates that the microsatellite markers used may be capable of discriminating between related and unrelated scallop larvae in the absence of pedigree information,and of investigating the effective number of parents contributing to the hatchery population of the Japanese scallop.

Method and Progress of the Research on Virus Affinity Peptide

With the continuous progress on affinity peptide research, it has become more and more popular in pharmacology and medicine. lt is promising to study these viruses affinity peptide to treat infectious diseases. And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection, treatment as wel as the study on the molecular mechanism of virus affinity peptide. Therefore, we reviewed the bioinformatics pre-diction technologies of computer simulation, molecular docking and homology model-ing, as wel as the research method on analyzing and screening virus affinity pep-tide, such as Phage display technology.

Adsorption and Step Elution of Urokinase Using Affinity Chromatography-Comparison of Data with Rate Model Simulation

A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.

Expression, purification and activity assay of recombinant human thymidylate synthase

Thymidylate synthase （TS, E.C.2.1.1.45） catalyzes a critical reaction in the only pathway of de novo synthesis of thymidylate （dTMP） in human cells, and is an important target of chemotherapy. To evaluate the inhibitory activities of novel compounds to TS, a convenient method of activity assay using 6x His-tagged recombinant human TS （rhTS） was established and 49 novel synthetic folate analogues were screened to discover potential TS inhibitors. During the process, 4 novel compounds were found to effectively inhibit TS, while the IC 50 of a positive control raltitrexed was 3.4 μM in this assay.

Oxidative cross-coupling: an alternative way for C–C bond formations

Coupling reaction usually refers to the direct C–C bond formation between two carbon fragments.Generally, cross-coupling reactions between nucleophiles and electrophiles have been extensively studied and become the classic model for bond constructions. Another reaction model, bond formation from two nucleophiles through oxidative cross-coupling, has received more and more attention over the past few years. This paper will discuss the concept of oxidative cross-coupling and give an overview of its recent development.