以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋...以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。展开更多
以自合成的5.0μm单分散大孔亲水交联聚甲基丙烯酸环氧丙酯(PGMA-EDMA)微球为基质,与亚氨基二乙酸键合,合成了亲水性阳离子色谱固定相。用3.5 mmol/L甲烷磺酸作淋洗液,在流速为1.0 m L/min下,对二乙醇胺、Na+、NH4+、K+混合样及有机胺...以自合成的5.0μm单分散大孔亲水交联聚甲基丙烯酸环氧丙酯(PGMA-EDMA)微球为基质,与亚氨基二乙酸键合,合成了亲水性阳离子色谱固定相。用3.5 mmol/L甲烷磺酸作淋洗液,在流速为1.0 m L/min下,对二乙醇胺、Na+、NH4+、K+混合样及有机胺和铵离子混合样进行分离。将其用于黄纸板中阳离子的分离测定,与原子吸收法(AAS)对照,测定结果基本一致。展开更多
Using the industrial technologies of rubber latex irradiation,preparation of nanoscale silica(SiO2) slurry,mixing irradiated rubber latex with SiO2 slurry,and the spray drying,we have prepared the ultrafine fully-vulc...Using the industrial technologies of rubber latex irradiation,preparation of nanoscale silica(SiO2) slurry,mixing irradiated rubber latex with SiO2 slurry,and the spray drying,we have prepared the ultrafine fully-vulcanized powder carboxyl styrene-butadiene rubber(UFPCSBR)/SiO2 nanocompound powder,in which the SiO2 particles and UFPCSBR particles are isolated and stuck each other.When the UFPCSBR/SiO2 nanocompound powder is mixed with crude rubber,the UFPCSBR particles are dispersed well in rubber matrix because of their good compatibility,then the SiO2 particles are also dispersed well in rubber matrix because of the carrier nature of the UFPCSBR particles during the mixing procedure,and the novel rubber/UFPCSBR/SiO2 nanocomposites are fabricated.Compared with the rubber composites prepared by mixing the crude rubber with the UFPCSBR powder and SiO2 powder one after the other,the novel UFPCSBR/SiO2 nanocompound modified rubber/UFPCSBR/SiO2 nanocomposites have better abrasion resistance,higher tensile strength and tear strength,and lower heat build-up data.Noteworthily,the tanδ-temperature curve of the novel rubber/UFPCSBR/SiO2 nanocomposites has the second tanδ peak due to the newly generated boundary layer surrounding the SiO2 particles,increasing the tanδ values in the temperature range of 0-20℃,which is very important to the research of green tyre tread.展开更多
文摘以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。
文摘以自合成的5.0μm单分散大孔亲水交联聚甲基丙烯酸环氧丙酯(PGMA-EDMA)微球为基质,与亚氨基二乙酸键合,合成了亲水性阳离子色谱固定相。用3.5 mmol/L甲烷磺酸作淋洗液,在流速为1.0 m L/min下,对二乙醇胺、Na+、NH4+、K+混合样及有机胺和铵离子混合样进行分离。将其用于黄纸板中阳离子的分离测定,与原子吸收法(AAS)对照,测定结果基本一致。
基金supported by the National Natural Science Foundation of China (Grant No. 50873049)National High Technology Research and Development Program of China ("863" Program) (Grant No. 2009AA-03Z338)+1 种基金Shandong Young Scientists Encouragement Foundation (Grant No. 2007BS04038)the Scientific Research Foundation of Shandong Education Department (Grant No. J07YA12)
文摘Using the industrial technologies of rubber latex irradiation,preparation of nanoscale silica(SiO2) slurry,mixing irradiated rubber latex with SiO2 slurry,and the spray drying,we have prepared the ultrafine fully-vulcanized powder carboxyl styrene-butadiene rubber(UFPCSBR)/SiO2 nanocompound powder,in which the SiO2 particles and UFPCSBR particles are isolated and stuck each other.When the UFPCSBR/SiO2 nanocompound powder is mixed with crude rubber,the UFPCSBR particles are dispersed well in rubber matrix because of their good compatibility,then the SiO2 particles are also dispersed well in rubber matrix because of the carrier nature of the UFPCSBR particles during the mixing procedure,and the novel rubber/UFPCSBR/SiO2 nanocomposites are fabricated.Compared with the rubber composites prepared by mixing the crude rubber with the UFPCSBR powder and SiO2 powder one after the other,the novel UFPCSBR/SiO2 nanocompound modified rubber/UFPCSBR/SiO2 nanocomposites have better abrasion resistance,higher tensile strength and tear strength,and lower heat build-up data.Noteworthily,the tanδ-temperature curve of the novel rubber/UFPCSBR/SiO2 nanocomposites has the second tanδ peak due to the newly generated boundary layer surrounding the SiO2 particles,increasing the tanδ values in the temperature range of 0-20℃,which is very important to the research of green tyre tread.
