高危型人乳头瘤病毒感染影响因素及其与阴道微生态系统的关联分析

目的分析与高危型人乳头瘤病毒(HR-HPV)感染相关的影响因素,并判断其与阴道微生态系统的关联性。方法将我院2023年1月至2024年1月110例进行筛查的女性作为研究对象,根据HR-HPV感染情况分为感染组(33例)与未感染组(77例),分析HR-HPV感染的影响因素分析,对2组研究对象阴道微生态进行比较,分析HR-HPV感染与阴道微生态的关联。结果感染组患者初次性生活年龄≤20岁、性伴侣人数≥2人、非避孕套避孕方式、乳酸杆菌比例异常、细菌性阴道病阳性占比均高于非感染组,差异有统计学意义(P<0.05);以单因素分析中差异有统计学意义的资料为自变量,HR-HPV感染为因变量,经多因素logistic回归分析显示,初次性生活年龄≤20岁、性伴侣人数≥2岁、非避孕套避孕方式、乳酸杆菌比例异常、细菌性阴道病阳性为HR-HPV感染的独立危险因素[OR值(95%CI)分别为:2.273(1.347,3.836)、2.370(1.537,3.655)、2.289(1.447,3.621)、2.252(1.427,3.556)、2.309(1.404,3.799),P均<0.05]。结论初次性生活年龄≤20岁、性伴侣人数≥2人、非避孕套避孕方式为HR-HPV感染的独立危险因素,乳酸杆菌比例异常、细菌性阴道病可能促进HR-HPV的感染。

Human papillomavirus in upper digestive tract tumors from three countries

AIM: To clarify human papillomavirus (HPV) involvement in carcinogenesis of the upper digestive tract of virological and pathological analyses. METHODS: The present study examined the presence of HPV in squamous cell carcinomas of the oral cavity (n = 71), and esophagus (n = 166) collected from Japan, Pakistan and Colombia, with different HPV exposure risk and genetic backgrounds. The viral load and physical status of HPV16 and HPV16-E6 variants were examined. Comparison of p53 and p16INK4a expression in HPV-positive and HPV-negative cases was also made. RESULTS: HPV16 was found in 39 (55%) oral carcinomas (OCs) and 24 (14%) esophageal carcinomas (ECs). This site-specific difference in HPV detection between OCs and ECs was statistically significant (P < 0.001). There was a significant difference in the geographical distribution of HPV16-E6 variants. Multiple infections of different HPV types were found in 13 ECs, but multiple infections were not found in OCs. This difference was statistically significant (P = 0.001). The geometric means (95% confidence interval) of HPV16 viral load in OCs and ECs were 0.06 (0.02-0.18) and 0.12 (0.05-0.27) copies per cell, respectively. The expression of p16INK4a proteins was increased by the presence of HPV in ECs (53% and 33% in HPV-positive and-negative ECs, respectively; P = 0.036), and the high-risk type of the HPV genome was not detected in surrounding normal esophageal mucosa of HPV-positive ECs. CONCLUSION: Based on our results, we cannot deny the possibility of HPV16 involvement in the carcinogenesis of the esophagus.

HPV 6b L1VIRUS-LIKE PARTICLES ELICIT HUMORAL IMMUNITY IN MICE

Objective.To test whether intramuscular,intranasal,intrarectal and intravagina l administration of HPV6b L 1 virus-like particlescould induce immune response in mice and to as sess whether intra-muscular and mucosal vaccination against HPV is feasible.Me thods.HPV6b L1proteins self-assembled into VLPs in Sf-9cell in vitro.Mic e were immunized on day0and21with50ìg HPV6b L1VLPs intramuscularly,int ranasally,intrarectally and intravagi-nally respectively.Sera were collected for testing IgG titer after a further7days and3months respec-tively.Results .After immunizations,all mice developed significant anti-HPV6b L1antibody titers in serum by7days after the second immunization.The titer of the serum I gG antibody against HPV6b L1VLPs in the intramuscularly immunized group was h igher than that in the intranasally,intrarectally and intravaginally immunized groups respectively,indicating that both muscular and mucosal administration of HPV6b L1VLPs can stimulate a systemic HPV-specific antibody response.Sera of the mice in the in-tramuscularly immunized group still maintained a high tit er of the serum IgG antibody against HPV6b L1VLPs 3months after the immunizat ion.Conclusion.The results demonstrated that the HPV6b L1VLPs maintain stro ng antigenicity.Immu-nization with HPV6b L1VLPs via intramuscular and mucos al routes,without adjuvant ,can elicit spe-cific antibody in sera.These fin dings suggest that the VLPs are able to induce protective antibodies.

Detection of Human Papillomaviral Infection on Kazakh Patients with Esophageal Squamous Cell Carcinoma in Xinjiang

OBJECTIVE To investigate the detection rate of humanpapilloma virus (HPV) DNA in the Kazakh esophageal carcinoma(EC) patients of Xinjiang.METHODS We detected the prevalence of a HPV gene in tumortissues from 318 esophageal squamous cell carcinoma (ESCC).Tumor tissues were kept in formalin and embedded in paraffin.One hundred seventeen samples used crude cell suspension, whilethe other 201 used the method of DNA extraction with phenol-Tris/chloroform. We analyzed the relevance to EC of Kazakh's inXinjiang.RESULTS In the ESCC samples of Kazakh's in Xinjiang, totaldetection rate for HPV DNA was 64.5% (205/318). The positiverate of HPV in group of crude cell suspensions was 82.9% (97/117)compared with the rate of 53.7% (108/201) in the group of DNAextraction. The results in the two groups showed significantdiffference (x^2 = 5.711, P < 0.05).CONCLUSION HPV DNA infection may be one of the mostimportant factors related to EC of Kazakh's in Xinjiang.

Etiology and Prevention of Recurrent Condyloma Accuminata

The possible causes of condyloma acuminatarecurrence arc summarized: such as patients with cellularimmune denciencies, physical therapy triggering subclinicalinfective foci, sowing of the virus particles, and insufficienttherapy. Corresponding preventive measures are addressed,including: immunomodulators improving cellular immunity,ensuring the range and depth of physical therapy, and trcatingsexual partners simultaneously.

Fluorescence Quantitative PCR Detected Infection of Condyloma Acuminatum

Objective: Infection of human papillomavirus in condylomaacuminatum (CA) was detected by real time fluorescencequantitative PCR (FQ-PCR) technique. Methods: Specimens of CA-DNA quantification from 94cases were examined by real time FQ-PCR technique and 32cases were compared with the same method after 10-daystreatment. Results: CA-DNA was found in all patients, with an averageof 4.0×10^6 copies/ul. After 10 days of treatment, the averagewas 2.1×10^5 copies/ul. There was a significant difference inthe average amount of CA-DNA before and after thetreatment. Conclusion: Real time FQ-PCR is a good method forexamining CA-DNA amount and it can direct the treatment of CA.

Relationship Between Apoptosis and PCNA Expression of Keratinocytes in Condylomata Acuminata

Objecrive: To investigate the relationship between apoptosis and proliferating cell nuclear antigcn (PCNA)expression of keratinocytes in Condylomata acuminata (CA). Methods: PCNA expression was observed byimmunohistochemistry technique (ABC method) in 51 CAspecimens and 1 normal specimens of foreskin or vaginalmucosae. 55 specimens (40 in the CA group and 15 in thecontrol group) were randomly sampled for in situ labelingof apoptotic cells using the TUNEL method. Results: Positive expression of PCNA in CA and controlgroups were 90.2% and 77.8%, respectively, and theproliferation index in CA group was significantly higherthan that in the control group (P<0.001). The positive rateof apoptosis was 42.5% in the LA group and 53.3% in thecontrol group, and there were no significant differences inthe apoptotic index and apoptosis-proliferation ratiobetween two groups (P>0.05). The proliferation indexshowed a significant negativc correlation with theapoptosis-proliferation ratio (r=-0.62, P=0.01) in the CAgrp. Conclusion: It is suggested that the proliferativeappearance of CA could be due to the imbalance betweencell growth and cell death which is caused by moreproliferation and less apoptosis in keratinocytes.