人健康肝组织核心岩藻糖基化蛋白质的鉴定及生物信息学分析

糖蛋白质组学方法鉴定人健康肝组织核心岩藻糖基化蛋白质,将有助于发现肝癌、慢性肝病相关异常核心岩藻糖基化蛋白质.应用凝集素亲和层析技术、双向电泳(2-DE)联合基质辅助激光解吸飞行时间串联质谱(MALDI-TOF-MS/MS)分析,建立人健康肝组织核心岩藻糖基化蛋白表达谱,图谱均点数为(130±3)个,挖取90个蛋白质点进行质谱鉴定,数据库搜索及去冗余后,共鉴定53种蛋白质,主要分布于pI5～9,分子质量为10～100ku区域处.Gene Ontology分类发现它们参与体内代谢等过程,应用NetNGlyc对它们进行糖基化位点预测,并通过蛋白质免疫沉淀联合凝集素亲和印迹对其中的结合珠蛋白前体,α烯醇化酶的核心岩藻糖基化进行了再验证.以上结果提示,凝集素层析、2-DE联合MALDI-TOF-MS/MS分析是一种鉴定某种特定类型糖蛋白的高通量检测方法,所建立的表达谱可用于发现疾病发生、发展中相关的异常核心岩藻糖基化蛋白质.

Calbindin-D28k与肌动蛋白在人健康及龋坏牙齿中的分布

目的 探讨Calbindin -D2 8k与肌动蛋白在健康和龋坏人恒牙中的分布相关性及意义。方法 用免疫组化方法分别检测Calbindin -D2 8k与肌动蛋白在正常、中龋和深龋人牙中的表达并观察比较。结果 正常组Calbindin -D2 8k在前期牙本质远髓 2 / 3区域染色阳性 ,肌动蛋白在成牙本质细胞及位于矿化牙本质中的细胞突呈阳性 ;中龋组位于前期牙本质中的成牙本质细胞突Calbindin -D2 8k与肌动蛋白均呈强阳性 ,Calbindin -D2 8k在病损区前期牙本质全层呈阳性且较正常组明显增强 ;深龋组Calbindin -D2 8k染色部位主要位于成牙本质细胞核。结论 在第三期牙本质形成活跃的成牙本质细胞中Calbindin -D2 8k与肌动蛋白的分布变化具有一致性 ,均与牙本质基质的分泌有关。

结核病人健康教育的研究

我国的结核疾病发病患者很多,在世界22个此疾病的高发国家中,我国发生此疾病的人数排名为第2位。我国现在大概有5亿左右的人受到结核的感染,有450万例患者为肺结核的患者。痰涂片阳性肺结核患者大概有65万例左右。我国结核病患者的耐药性很高，高达18．6％左右。肺结核是慢性病，治疗时间长，要治愈必须早期、联合、适量、规律、和全程治疗。

上海社区老年人健康促进生活方式影响因素分析

目的:研究上海社区老年人健康促进生活方式现状及其影响因素。方法:采用一般情况调查表和中文版老年人健康促进生活方式量表对上海社区老年人基本情况和生活方式现状进行调查。结果:老年人健康促进生活方式量表平均得分为(96.63 ±16.91)分。各量表得分依次为:饮食营养维度得分为(13.06 ±2.00)分,运动锻炼维度得分为(10.22 ±2.61)分,精神成长维度得分为(14.17 ±3.34)分,健康责任维度得分为(22.08 ±5.59)分,压力应对维度得分为(19.24 ±3.96)分,人际关系维度得分为(17.86 ±3.03)分。年龄、健康关注度、健康自我效能、公共健康设施健全程度和公共健康服务开展情况对老年人健康促进生活方式存在显著影响。结论:上海社区老年人健康促进生活方式水平为中等偏上,可通过提升健康自我效能、增加健康关注度、开展多种公共健康服务,健全公共健康设施来提高老年人健康促进生活方式水平,从而改善其健康状况。

健康城市主动式规划干预途径研究

在全球健康城市战略背景下,以“城市系统健康”和“人健康”为核心的整体研究范式是健康城市规划的核心议题,预防、预测为主的主动式健康干预成为重要新趋向。为此,本文立足于城乡规划的干预-约束职能,关注城市空间环境对公共健康的主动式干预作用,提出健康城市主动式规划干预概念分析框架,从健康风险评估及优化、公共健康资源合理配置和健康生活方式引领3方面提出规划思路。旨在获取城市空间环境对健康城市建设的最优效益,采取适应规避、合理分配和引导的规划思路实现超前干预,将对健康城市社会组织建构、利益相关者参与和战略制定的思考转译为切实可操作的规划策略,提升城市空间环境对公共健康的干预落地实效力。

人健康肝组织刀豆凝集素(Con A)结合型糖蛋白组学研究

通过凝集素亲和层析技术结合双向电泳-质谱技术构建人健康肝组织Con A结合型糖蛋白数据库并进行生物信息学分析;利用研磨和匀浆两步法抽提人健康肝组织总蛋白,通过固相凝集素亲和层析获得与刀豆凝集素(Con A)结合型糖蛋白,经过双向电泳、荧光染色、图像分析获得人健康肝组织表达谱;通过MALDI-TOF-MS质谱鉴定及生物信息学分析获得人健康肝组织数据库,并建立了高重复性的人健康肝组织Con A结合型糖蛋白表达谱,图谱均点数为301±15,挖取139个蛋白点进行质谱鉴定,去冗余后确定了85个与细胞周期、代谢通路相关的蛋白质;通过NetNGlyc和NetOGlyc对糖基化位点进行预测,并按照geneontology对其功能定位等进行分类,并对其中与肿瘤发生发展相关的蛋白进行具体分析.由此可见,凝集素亲和层析结合2-DE,质谱鉴定是一种高通量的检测方法,Con A结合型糖基化修饰谱的构建为后续研究奠定了基础.

Pharmacokinetics of Oxiracetam in Healthy Volunteers

Aim To study the pharmacokinetics of oxiracetam after single and multipleintravenous administrations in healthy volunteers. Method A HPLC method was used to determine theserum concentration of oxiracetam after intravenous single dose and daily dose of 2 000 mg for 7 din ten Chinese healthy volunteers. Pharmacokinetic analysis was carried out using Drug And Statisticsoftware. Results The AUC_(0-12), AUC_(0-∞), K_e, t_(1/2), MRT after a single dose of 2 000 mgoxiracetam were 256.26 ± 16.84 μg·mL^(-1)·h, 276.74 ±18.11 μg·mL^(-1)·h, 0.18 ±0.03 h^(-1),3.84±0.64 h, and 4.39 10.39 h, and after multiple doses of oxiracetam were 259.36 ±25.43μg·mL^(-1)·h, 285.59 ±27.38 μg·mL^(-1)·h, 0.17 ±0.04 h^(-1), 4.14 ± 0.82 h, and 4.87 ±0.69 h, respectively. Conclusion The pharmacokinetic parameters of oxiracetam do not differremarkably after single and multiple intravenous administration and there is accumulation in serumafter 2 000 mg multiple intravenous administration once a day fof 7 d.

An Evaluation of STD/AIDS Health Education among Female Sex Workers in Entertainment Establishments in Jingjiang City

Objective: To explore effective ways of conducting STD/AIDS health education among female commercial sex workers in entertainment establishments and to promote implementation of an 100% condom use programme. Methods: In-depth interviews were carried out to collect qualitative information about demographic characteristics, STD/AIDS knowledge, attitude and risk behaviors of female sex workers. Female sex workers were selected by sampling and were given baseline survey and assessment after intervention. Based on the results of the interviews, a questionnaire was developed, and intervention measures were determined. These measures included face-to-face interviewing, counseling, and distribution of STD/AIDS information and condoms. Results: 196 and 182 female sex workers of the same population were interviewed separately before and after intervention. STDs/AIDS knowledge had significantly increased after one year's intervention (P <0.01). Knowledge increased in both the intervention group and the non-intervention group but the increase was more significant among sex workers who received the intervention. Risk behaviors remained at baseline levels in both groups. Conclusion: STD/AIDS health education among female sex workers in entertainment establishments was effective and may benefit society, but the strategies need to be adjusted to the female sex workers' lifestyles, particularly their high rates of mobility and high-risk behaviors.

Effect of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study

AIMTo evaluate the ability of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 to colonize the intestinal environment of healthy subjects and modify the gut microbiota composition.METHODSTwenty healthy Italian volunteers, eight males and twelve females, participated in the study. Ten subjects took a sachet containing 4 × 109 colony-forming units (CFU) of Bifidobacterium longum BB536 and 109 CFU of Lactobacillus rhamnosus HN001, 30 min before breakfast (pre-prandial administration), while ten subjects took a sachet of probiotic product 30 min after breakfast (post-prandial administration). The ability of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 to colonize human gut microbiota was assessed by means of quantitative real-time PCR, while changes in gut microbiota composition were detected by using Ion Torrent Personal Genome Machine.RESULTSImmediately after 1-mo of probiotic administration, B. longum BB536 and L. rhamnosus HN001 load was increased in the majority of subjects in both pre-prandial and post-prandial groups. This increase was found also 1 mo after the end of probiotic oral intake in both groups, if compared to samples collected before probiotic consumption. At phyla level a significant decrease in Firmicutes abundance was detected immediately after 1-mo of B. longum BB536 and L. rhamnosus HN001 oral intake. This reduction persisted up to 1 mo after the end of probiotic oral intake together with a significant decrease of Proteobacteria abundance if compared to samples collected before probiotic administration. Whereas, at species level, a higher abundance of Blautia producta, Blautia wexlerae and Haemophilus ducrey was observed, together with a reduction of Holdemania filiformis, Escherichia vulneris, Gemmiger formicilis and Streptococcus sinensis abundance. In addition, during follow-up period we observed a further reduction in Escherichia vulneris and Gemmiger formicilis, together with a decrease in Roseburia faecis and Ruminococcus gnavus abundance. Conversely, the abundance of Akkermansia muciniphila was increased if compared to samples collected at the beginning of the experimental time courseCONCLUSIONB. longum BB536 and L. rhamnosus HN001 showed the ability to modulate the gut microbiota composition, leading to a significant reduction of potentially harmful bacteria and an increase of beneficial ones. Further studies are needed to better understand the specific mechanisms involved in gut microbiota modulation.

Dignity and its related factors among older adults in long-term care facilities:A cross-sectional study

Objectives:This study aimed to explore the dignity and related factors among older adults in long-term care facilities.Methods:Cross-sectional data were obtained from a sample of 253 Chinese older adults dwelling in long-term care facilities.Dignity among older adults was measured using the Dignity Scale,and its potential correlates were explored using multiple linear regressions.Results:Results showed that the total score of the Dignity Scale is 151.95±11.75.From high to low,the different factors of dignity among older adults in long-term care facilities were as follows:caring factors(4.83±0.33),social factors(4.73±0.41),psychological factors(4.66±0.71),value factors(4.56±0.53),autonomous factors(4.50±0.57),and physical factors(4.38±0.55).A higher score of the Dignity Scale was associated with higher economic status,fewer chronic diseases,less medication,better daily living ability and long-time lived in cities.Conclusion:Older adults with low economic status,more chronic diseases,and poor daily living ability,taking more medications,or the previous residence in rural areas seem to be most at low-level dignity in long-term care facilities and thus require more attention than their peers.

Surface water quality and potential health risk assessments in Changsha-Zhuzhou-Xiangtan section of Xiangjiang River, China

The Changsha-Xiangtan-Zhuzhou City Group is a heavy industrial district and accepted as the serious pollution area in the Xiangjiang River basin.In this study,7 metals(Pb,Hg,Cd,As,Zn,Cu and Se)and the river water quality parameters including pH,dissolved oxygen(DO),Escherichia coli(E.coli),potassium permanganate index(CODMn),dichromate oxidizability(CODCr),five-day biochemical oxygen demand(BOD5),ammonia nitrogen(NH4+-N),total nitrogen(TN),total phosphorus(TP)and fluoride(F)in 18 sampling sites of the Changsha-Xiangtan-Zhuzhou section are monthly monitored in 2016,which is the year to step into the second stage of the“Xiangjiang River Heavy Metal Pollution Control Implementation Plan”.It is found that E.coli,TN and TP are the main pollutants in the Changsha-Zhuzhou-Xiangtan section,and the pollution of heavy metal is not serious but As with potential risk to local people especially children should be concerned.In addition,Xiangtan city is mainly featured with heavy metal pollution,while Zhuzhou and Changsha city are both featured with other pollutants from municipal domestic sewage.

Systematic review of Chinese herbal medicine for functional constipation

Constipation is a common gastrointestinal complaint in clinical practice, affecting an estimated 27% of the population. Many patients are disappointed by current conventional treatments and, therefore, seek help from complementary and alternative medicine （CAM）. Traditional Chinese medicine, is the most important part of CAM and has been practiced for treating diseases and promoting the health of humans for thousands of years, and has become a popular alternative choice. Although there are many Chinese herbal medicine （CHM interventions available, and some have been verified b＇ clinical trials, their efficacy and safety are still questioned by both patients and health care providers worldwide. The purposes of this review are, first, to appraise the qualities of individual study designs in the new Cochrane approach. Second, the benefits of individual CHM interventions or individual types of CHM intervention for the treatment of functional constipation are analyzed. Finally, valid and comprehensive conclusions are drawn, if applicable, in order to make clinical recommendations.

Pedogeochemical Environments and Peoples Health in China

It has long been recognized that pedogeochemistry is closely related with human health. Based on analytical data, the weathering or soil forming processes fall into four stages and China is divided into four geochemical regions: saline soil region, carbonate soil region, siallitic soil region and ferrallitic soil region. Moreover, some endemic diseases in relation to the deficit or excess of some elements are discussed in the viewpoint of pedogeochemistry.

Assessment of water quality and safety based on multi-statistical analyses of nutrients, biochemical indexes and heavy metals

The purpose of this research was to better understand the water quality status of the Xiangjiang River, China, and to evaluate the risks posed by the river water. Precisely, ten water quality parameters including p H, dissolved oxygen(DO), Escherichia coli(E. coli), potassium permanganate index(CODMn), dichromate oxidizability(CODCr), five-day biochemical oxygen demand(BOD5), ammonia nitrogen(NH4+-N), total phosphorus(TP) and fluoride(F-) as well as metal(loid)s(Pb, Hg, Cd, As, Zn, Cu and Se) were monitored monthly in 2016 at 12 sampling sites throughout the Hengyang section of the Xiangjiang River. Concentrations of all parameters were presented according to rainy and dry seasons. They were compared with Chinese surface water standards and WHO drinking water limits to assess the sustainability of the river water status. Principal component analysis(PCA) revealed different pollution sources in different seasons. Dual hierarchical cluster analysis(DHCA) was applied to further classify the water quality variables and sampling sites. Besides, a risk assessment was introduced to evaluate the carcinogenic and non-carcinogenic concerns of heavy metal(loid)s to human health. This research will help to optimize water monitoring locations and establish pollution reduction strategies on the preservation of public safety.

Recent advances and remaining gaps in our knowledge of associations between gut microbiota and human health

The complex gut microbial flora harbored by individuals(microbiota) has long been proposed to contribute tointestinal health as well as disease. Pre-and probioticproducts aimed at improving health by modifyingmicrobiota composition have already become widelyavailable and acceptance of these products appearsto be on the rise. However, although required forthe development of effective microbiota basedinterventions, our basic understanding of microbiotavariation on a population level and its dynamics withinindividuals is still rudimentary. Powerful new parallelsequence technologies combined with other efficientmolecular microbiota analysis methods now allow forcomprehensive analysis of microbiota composition inlarge human populations. Recent fi ndings in the fi eldstrongly suggest that microbiota contributes to thedevelopment of obesity, atopic diseases, inflammatorybowel diseases and intestinal cancers. Through theongoing National Institutes of Health Roadmap 'HumanMicrobiome Project' and similar projects in other partsof the world, a large coordinated effort is currentlyunderway to study how microbiota can impact humanhealth. Translating findings from these studies intoeffective interventions that can improve health,possibly personalized based on an individuals existingmicrobiota, will be the task for the next decade(s).

Yoga: A tool for improving the quality of life in chronic pancreatitis

AIM： To determine the effectiveness of yoga on improving the quality of life in patients of chronic pancreatitis. METHODS： The patients were randomized to two groups. The control group continued their usual care as directed by their physicians. Patients in the yoga group, in addition, received biweekly yoga sessions for 12 wk. The patients＇ demographic and health behaviour variables were assessed before and alter the yoga programme using Medical Outcomes Short Form （SF-36） for quality of life, Profile of Mood States for assessing mood and Symptoms of Stress Inventory for measuring stress. RESULTS： A total of 60 patients were enrolled, with 8 drop-outs.Thirty patients were randomized to the yoga group and 30 to the control group. Significant improvements were seen in overall quality of life, symptoms of stress, mood changes, alcohol dependence and appetite alter the 12 wk period apart from the general feeling of well-being and desire to continue with the programme in future in the yoga group, while there was no difference in the control group. CONCLUSION： Yoga is effective on improving the quality of life in patients of chronic pancreatitis.

Social isolation and health-promoting behaviors among older adults living with different health statuses:A cross-sectional study

Objectives:To describe the characteristics and relationships of social isolation and health promoting behaviors of Chinese older adults with different health statuses.Methods:Totally 485 older adults were recruited.They were divided into three groups based on their health status:healthy group(n=72),group with one disease(n=183)and group with multimorbidity(n=230).Lubben Social Network Scale-6 and Health-Promoting Lifestyle Profile II were used to measure their social isolation and health-promoting behaviors,respectively.Results:Among the aged with one disease or multimorbidity,30.6%(56/183)and 31.7%(73/230)were socially isolated,respectively,based on the score of the Lubben Social Network Scale-6.The three groups differed significantly in health-promoting behaviors and scores on several subscales.The group with multimorbidity exhibited more social isolation and fewer health-promoting behaviors than other groups.Social isolation was among the factors adversely affecting health-promoting behaviors in older adults.Conclusions:Health care providers should help older adults with multimorbidity preserve the remaining abilities to improve health-promoting behaviors.Meanwhile,it is important to support healthy older adults to increase self-responsibility to achieve healthy aging.

Exercise is more than medicine: The working age population's well-being and productivity

Background： Physical activity （PA） includes muscle activity during exercise, manual work, and leisure time activities including sport. Conflicting results exist regarding health effects of PA that may deteriorate with manual work and elite sports, but improve when performed in moderation in accordance with international guidelines and may additionally enhance well-being and productivity. Methods： In Denmark 15 randomized controlled trials have been conducted, introducing exercise at the workplace enrolling 〉3500 workers. The interventions lasted from 10 to 52 weeks and offered -1 h weekly supervised exercise during working hours according to the concept of intelligent physical exercise training （IPET） that is based on evidenced sports sciences training principles and tailored to work exposure, employee health status, and physical capacity. Questionnaire surveys and health checks including blood and muscle sampling were performed at baseline and follow-up. The job groups included： office and computer workers, dentists, industrial technicians, cleaning personnel, health care workers, construction workers, and fighter/helicopter pilots. Results： In all job groups significant improvements were documented regarding health outcomes. These were job group specific： neck pain was reduced among office and computer workers, dentists, industrial laboratory technicians, health care workers as well as fighter pilots. Cardio- respiratory fitness--a health risk indicator for cardio-metabolic diseases--was improved among office and computer workers, health care workers, and construction workers. Additionally, other improvements were evidenced such as increased muscle strength and balance control. Importantly, productivity increased with improved muscle strength and decreased body mass index. Conclusion： IPET does enhance health if an exercise program with evidenced efficacy is implemented by expert trainees with support of the employer. Accordingly, in every study group outcomes of improved health were documented and the effect sizes were of clinical relevance. Cost effectiveness estimates indicate acceptable cost relative to savings on health expenses and lost productivity.

Quantifying the emission＇s impact of coal mining activities on the environment and human health in process

Discharges and emissions in the coal mining process have a strong effect both on the environment and on human health. This problem is usually be a negative one and has only been recognized qualitatively, due to the lack of effective quantitative methods. Based on emergy theory and accounting methodology, a set of quantitative methods for accounting the environmental support due to pollutants emissions was first introduced. Then impacts on environment and effects on human health were quantified using the unified units. The results indicated that water pollutants caused more impacts on the environment than air pollutants did, i.e., more environmental contributions are needed to dilute and absorb water pollutants. The occupation of land caused by coal mining gangue waste stacking has led to a huge loss of environmental services over the years. Moreover, the potential damage on the human condition health caused by CO2 through climate change cannot be ignored. Finally, the impacts of mining activities on environmental and human health in unified units are shown to provide a quantitative insight into the disadvantage of coal mining. The comparable results of the method indicate the different influence of various pollutants and the contribution of ＇natural capital＇ directly. This work is a part of ongoing thermodynamic input-output analysis and life cycle analysis of coal mining systems （which are in process.）

Prevalence and correlates of lifestyle behavior,anxiety and depression in Chinese college freshman:A cross-sectional survey

Objectives:First-year college students had exposure to unhealthy lifestyle behaviors that correlate with a high prevalence of anxiety and depression.Regarding to the modifiable lifestyle behaviors factors,this study investigated the prevalence and correlation of multiple lifestyle behaviors,anxiety and depression in a sample of Chinese first-year college students.Methods:Cross-sectional data were extracted from Residents eHealth app of health lifestyle behaviors survey from September to October 2019.Anxiety,depression,eating regular meals,consumption of snacks in-between meals,consumption of fruit,dessert and sugar-sweetened beverages,smoking and secondhand smoke exposure,consuming alcohol,physical activity,sedentary time were assessed by self-report.Socio-demographic including age,gender,education,family income,religion,and health condition were captured.Logistic regression was used to explore the association of multiple lifestyle behaviors,anxiety and depression.Results:Totally 1,017 participants were included in the study.The prevalence of anxiety and depression(from mild to severe) were 40.3% and 45.3%,respectively.In multivariable analyses,religion (believe in Buddhism,OR =2.438,95%CI:1.097-5.421;believe in Christian,OR =5.886,95%CI:1.604-21.597),gender (Female,OR =1.405,95%CI:1.001-1.971),secondhand smoke exposure (OR =1.089,95%CI:1.001-1.184),and eating regular meals (OR =0.513,95%C1:0.346-0.759) were associated with anxiety.Family income (OR =0.732,95%CI:0.596-0.898),eating regular meals (OR =0.641,95%CI:0.415-0.990),frequency of breakfast (OR =0.813,95%CI:0.690-0.959),with a chronic disease (OR =1.902,95%CI:1.335-2.712),and consumption of nocturnal snack (OR =1.337,95%CI:1.108-1.612) were associated with depression.Conclusions:These results highlighted the need for early lifestyle behavior intervention,especially modifying diet patterns considering the background of religion,health condition,and social-economic status in first-year college students to improve their mental health.