A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was develope...A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was developed to systemi- cally study the rice seed quality control. Genome-wide analysis of the FST distribution showed that T-DNA insertions were positively correlated with expressed genes, but negatively with transposable elements and small RNAs. In addition, the recovered T-DNAs were preferentially located at the untranslated region of the expressed genes. More than 11 000 putative homozygous lines were obtained through multi-generations of planting and resistance screening, and measurement of seed quality of around half of them, including the contents of starch, amylose, protein and fat, with a nondestructive near-infrared spectroscopy method, identified 551 mutants with unique or multiple altered param- eters of seed quality. Analysis of the corresponding FSTs showed that genes participating in diverse functions, including metabolic processes and transcriptional regulation, were involved, indicating that seed quality is regulated by a complex network.展开更多
The alpine treeline ecotone is an important component of mountain ecosystems of the Nepal Himalaya; it plays a vital role in the livelihood of indigenous people,and provides ecosystem services. However,the region face...The alpine treeline ecotone is an important component of mountain ecosystems of the Nepal Himalaya; it plays a vital role in the livelihood of indigenous people,and provides ecosystem services. However,the region faces a problem of paucity of data on treeline characteristics at the regional and landscape scales. Therefore,we used Remote Sensing(RS),and Geographic Information Science(GIS) approaches to investigate cross-scale interactions in the treeline ecotone. Additionally,European Space Agency land cover map,International Center for Integrated Mountain Development(ICIMOD) land cover map,ecological map of Nepal,and United States Geological Survey Shuttle Radar Topography Mission-Digital Elevation Model were used to analyze treeline pattern at the regional scale. Digital Globe high-resolution satellite imagery of Barun(eastern Nepal) and Manang(central Nepal) were used to study treeline patterns at the landscape scale. Treeline elevation ranges from 3300-4300 m above sea level. Abies spectabilis,Betula utilis,and Pinus wallichiana are the main treeline-forming species in the Nepal Himalaya. There is an east to west treeline elevationgradient at the regional scale. No slope exposure is observed at the regional scale; however,at the landscape scale,slope exposure is present only in a disturbed area(Manang). Topography and human disturbance are the main treeline controlling factor in Barun and Manang respectively.展开更多
When people realize or imagine that "if you have not taken the same choices as before, the result will be better", which kind of experience is called a regret emotion. The purpose of this study is to prove the relat...When people realize or imagine that "if you have not taken the same choices as before, the result will be better", which kind of experience is called a regret emotion. The purpose of this study is to prove the relationship between regret emotions and a sense of responsibility and control. The results show that the individual's sense of responsibility and regret that there is a positive correlation and there is a negative correlation between the control and regret, which is not match the previous part of the study so as to be further explored. At the same time, we found that the regret tendencies in the personality can be explained as some of the regret experiencing in the specific situation. When people realize or imagine that "if the choice is not the same as before that things are getting better now", they will feel regret.展开更多
The genetic resistance to angular leaf spot (ALS) caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived ...The genetic resistance to angular leaf spot (ALS) caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived from crosses between a resistant cultivar Mexico 54 and a susceptible cultivar Kablanketi under screen house conditions. The heritability (h2) estimate was as high as 0.719 indicating a successful transfer of ALS resistance among progenies and thus selection can be performed in early generation. High heritability coupled with high expected genetic advance of 39.5% is considered to be more useful in predicting the outcome of selecting the best individuals. Chi-square values were computed to determine whether the observed ratios for disease reactions deviated from expected Mendelian ratios for a single, dominant gene controlling resistance to angular leaf spot in common bean. Based on the resistance of the F2, and the backcross generation to the resistant parent, a 3 resistant: 1 susceptible segregation ratio in the F2 and a 1 resistant: 1 susceptible segregation ratio in the backcross generation to the susceptible parent was obtained implying that resistance to the isolate ofPhaeoisariopsis griseola is governed by a single, dominant gene.展开更多
Field experiments were conducted to study the inheritance of boron efficiency in oilseed rape (Brassica napus L.) by evaluating the boron (B) efficiency coefficient (BEC, the ratio of the seed yield at below the criti...Field experiments were conducted to study the inheritance of boron efficiency in oilseed rape (Brassica napus L.) by evaluating the boron (B) efficiency coefficient (BEC, the ratio of the seed yield at below the critical boron level to that at the boron-sufficient level) with 657 F2:3 lines of a population derived from a cross between a B-efficient cultivar, Qingyou 10, and a B-inefficient cultivar, Bakow. Qingyou 10 had high BEC as well as high seed yield at low available soil B. On the contrary, Bakow produced low seed yield at low B status. Boron deficiency decreased the seed yield of the F2:3 lines to different extents and the distribution of BEC of the population showed a bimodal pattern. When the 657 F2:3 lines were grouped into B-efficient lines and B-inefficient lines according to their BEC, the ratio of B-efficient lines to B-inefficient lines fitted the expected ratio (3:1), indicating that one major gene controlled the B-efficiency trait. 127 F2:3 lines selected from the population at random, with distribution of BEC similar to that of the overall population, were used to identify the target region for fine mapping of the boron efficiency gene.展开更多
The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5'-flanking regulatory elements of human β-globin gene, two negative control regions(NCR1,-610 to -490...The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5'-flanking regulatory elements of human β-globin gene, two negative control regions(NCR1,-610 to -490 bp;NCR2, -338 to-233bp), was identified.Two stage specific protein factors corresponding to embryonic and fetal stages were found to be capable of binding to NCR2.These data provided evidence that the cis acting elements of the 5'-flanking region might be involved in the developmental control of β-globin gene and NCR2 might be responsible in part for the silence of β-globin gene in the embryonic and fetal stages.展开更多
The purpose of this study was to examine the impact of macro social factors (states, religion, region, Arab spring, terrorism, unrest (Shoe index), democracy, corruption (GPI), Human development (HDI), low self...The purpose of this study was to examine the impact of macro social factors (states, religion, region, Arab spring, terrorism, unrest (Shoe index), democracy, corruption (GPI), Human development (HDI), low self-control, life stress events (LSE), youth unemployment, religiosity, feeling (fear and anger), youth unemployment and total unemployment) on Arab youth's radicalization. A sample of 6,730 Arab youth age 15-24 years was selected from Kuwait, UAE, KSA, Oman, Qatar, Bahrain, Jordan, Tunisia, Libya, Morocco, Lebanon, Egypt, Gaza and Palestine and Syrian refuges in Jordan. A questionnaire of 43 items to measure radicalization was developed based on the literature review. A construct validity of the scale was estimated by calculating the correlation between radicalization scale and Low self-control scale and found a positive significant relationship (0.680, a = 0.000), a sign of validity of the scale. A Reliability of the scale is strong and was estimated by Cronbach's alpha and was 0.947. An average of 46.6% of the participants was categorized as radicals with standard deviation of 12. Macro social factors explained 64% of the variance on radicalization. It has a significant impact on radicalization (F = 807.6, a = 0.000). Each single variable has a significant impact. The analysis revealed three groups of macro determinants of youth radicalization were identified: (1) Geographic factors: state, region, and Arab spring; (2) Social factors: religion, religiously, feelings, LSE and LSC; (3) Human security: unrest, terrorism, democracy, corruption, human development, youth unemployment and employment rate. To alleviate the consequences of radicalization, prevention policies should take in account youth concerns as partners and victims of radicalization. Policies need to focus on radicalization pull and push factors on micro-meso-macro level.展开更多
文摘A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was developed to systemi- cally study the rice seed quality control. Genome-wide analysis of the FST distribution showed that T-DNA insertions were positively correlated with expressed genes, but negatively with transposable elements and small RNAs. In addition, the recovered T-DNAs were preferentially located at the untranslated region of the expressed genes. More than 11 000 putative homozygous lines were obtained through multi-generations of planting and resistance screening, and measurement of seed quality of around half of them, including the contents of starch, amylose, protein and fat, with a nondestructive near-infrared spectroscopy method, identified 551 mutants with unique or multiple altered param- eters of seed quality. Analysis of the corresponding FSTs showed that genes participating in diverse functions, including metabolic processes and transcriptional regulation, were involved, indicating that seed quality is regulated by a complex network.
文摘The alpine treeline ecotone is an important component of mountain ecosystems of the Nepal Himalaya; it plays a vital role in the livelihood of indigenous people,and provides ecosystem services. However,the region faces a problem of paucity of data on treeline characteristics at the regional and landscape scales. Therefore,we used Remote Sensing(RS),and Geographic Information Science(GIS) approaches to investigate cross-scale interactions in the treeline ecotone. Additionally,European Space Agency land cover map,International Center for Integrated Mountain Development(ICIMOD) land cover map,ecological map of Nepal,and United States Geological Survey Shuttle Radar Topography Mission-Digital Elevation Model were used to analyze treeline pattern at the regional scale. Digital Globe high-resolution satellite imagery of Barun(eastern Nepal) and Manang(central Nepal) were used to study treeline patterns at the landscape scale. Treeline elevation ranges from 3300-4300 m above sea level. Abies spectabilis,Betula utilis,and Pinus wallichiana are the main treeline-forming species in the Nepal Himalaya. There is an east to west treeline elevationgradient at the regional scale. No slope exposure is observed at the regional scale; however,at the landscape scale,slope exposure is present only in a disturbed area(Manang). Topography and human disturbance are the main treeline controlling factor in Barun and Manang respectively.
文摘When people realize or imagine that "if you have not taken the same choices as before, the result will be better", which kind of experience is called a regret emotion. The purpose of this study is to prove the relationship between regret emotions and a sense of responsibility and control. The results show that the individual's sense of responsibility and regret that there is a positive correlation and there is a negative correlation between the control and regret, which is not match the previous part of the study so as to be further explored. At the same time, we found that the regret tendencies in the personality can be explained as some of the regret experiencing in the specific situation. When people realize or imagine that "if the choice is not the same as before that things are getting better now", they will feel regret.
文摘The genetic resistance to angular leaf spot (ALS) caused by Phaeoisariopsis griseola in the common bean cultivar Mexico 54 was investigated on disease reactions in parental, F1, F2 and backcross generations derived from crosses between a resistant cultivar Mexico 54 and a susceptible cultivar Kablanketi under screen house conditions. The heritability (h2) estimate was as high as 0.719 indicating a successful transfer of ALS resistance among progenies and thus selection can be performed in early generation. High heritability coupled with high expected genetic advance of 39.5% is considered to be more useful in predicting the outcome of selecting the best individuals. Chi-square values were computed to determine whether the observed ratios for disease reactions deviated from expected Mendelian ratios for a single, dominant gene controlling resistance to angular leaf spot in common bean. Based on the resistance of the F2, and the backcross generation to the resistant parent, a 3 resistant: 1 susceptible segregation ratio in the F2 and a 1 resistant: 1 susceptible segregation ratio in the backcross generation to the susceptible parent was obtained implying that resistance to the isolate ofPhaeoisariopsis griseola is governed by a single, dominant gene.
基金Project supported by the National Natural Science Foundation of China (No.30170549) the National High Tech-nology Research and Development Program (863 Program) of China (No.2004AA222190).
文摘Field experiments were conducted to study the inheritance of boron efficiency in oilseed rape (Brassica napus L.) by evaluating the boron (B) efficiency coefficient (BEC, the ratio of the seed yield at below the critical boron level to that at the boron-sufficient level) with 657 F2:3 lines of a population derived from a cross between a B-efficient cultivar, Qingyou 10, and a B-inefficient cultivar, Bakow. Qingyou 10 had high BEC as well as high seed yield at low available soil B. On the contrary, Bakow produced low seed yield at low B status. Boron deficiency decreased the seed yield of the F2:3 lines to different extents and the distribution of BEC of the population showed a bimodal pattern. When the 657 F2:3 lines were grouped into B-efficient lines and B-inefficient lines according to their BEC, the ratio of B-efficient lines to B-inefficient lines fitted the expected ratio (3:1), indicating that one major gene controlled the B-efficiency trait. 127 F2:3 lines selected from the population at random, with distribution of BEC similar to that of the overall population, were used to identify the target region for fine mapping of the boron efficiency gene.
基金gramts from shanghai Joint Laboratory of Life sciences,Academia Sinica,and the National National sciences Foundation,
文摘The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5'-flanking regulatory elements of human β-globin gene, two negative control regions(NCR1,-610 to -490 bp;NCR2, -338 to-233bp), was identified.Two stage specific protein factors corresponding to embryonic and fetal stages were found to be capable of binding to NCR2.These data provided evidence that the cis acting elements of the 5'-flanking region might be involved in the developmental control of β-globin gene and NCR2 might be responsible in part for the silence of β-globin gene in the embryonic and fetal stages.
文摘The purpose of this study was to examine the impact of macro social factors (states, religion, region, Arab spring, terrorism, unrest (Shoe index), democracy, corruption (GPI), Human development (HDI), low self-control, life stress events (LSE), youth unemployment, religiosity, feeling (fear and anger), youth unemployment and total unemployment) on Arab youth's radicalization. A sample of 6,730 Arab youth age 15-24 years was selected from Kuwait, UAE, KSA, Oman, Qatar, Bahrain, Jordan, Tunisia, Libya, Morocco, Lebanon, Egypt, Gaza and Palestine and Syrian refuges in Jordan. A questionnaire of 43 items to measure radicalization was developed based on the literature review. A construct validity of the scale was estimated by calculating the correlation between radicalization scale and Low self-control scale and found a positive significant relationship (0.680, a = 0.000), a sign of validity of the scale. A Reliability of the scale is strong and was estimated by Cronbach's alpha and was 0.947. An average of 46.6% of the participants was categorized as radicals with standard deviation of 12. Macro social factors explained 64% of the variance on radicalization. It has a significant impact on radicalization (F = 807.6, a = 0.000). Each single variable has a significant impact. The analysis revealed three groups of macro determinants of youth radicalization were identified: (1) Geographic factors: state, region, and Arab spring; (2) Social factors: religion, religiously, feelings, LSE and LSC; (3) Human security: unrest, terrorism, democracy, corruption, human development, youth unemployment and employment rate. To alleviate the consequences of radicalization, prevention policies should take in account youth concerns as partners and victims of radicalization. Policies need to focus on radicalization pull and push factors on micro-meso-macro level.