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兔成骨细胞与纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架生物相容性研究 被引量:2
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作者 吕宏 施杞 +4 位作者 王拥军 李家顺 叶晓健 蔡舒 张立德 《脊柱外科杂志》 2004年第4期209-212,共4页
目的 研究成骨细胞与纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架在体外培养条件下的生物相容性。方法 将成骨细胞株置于含体积分数为10%胎牛血清的DMEM培养基中培养,传代后改用含地塞米松、β-甘油磷酸钠和维生素C的条件培养基培养... 目的 研究成骨细胞与纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架在体外培养条件下的生物相容性。方法 将成骨细胞株置于含体积分数为10%胎牛血清的DMEM培养基中培养,传代后改用含地塞米松、β-甘油磷酸钠和维生素C的条件培养基培养,分为纳米支架复合细胞组和单纯细胞组,不同时间用倒置相差显微镜、HE染色光镜及扫描电镜观察。MTT法进行细胞增殖测定,并进行细胞微量蛋白含量检测和碱性磷酸酶的定量检测。结果 成骨细胞体外培养时复合或不复合纳米支架均生长良好,表现出典型的细胞形态特征和生物学特性,纳米支架利于细胞的贴附、生长与增殖,并对细胞的功能无不良影响。结论 纳米支架是较理想的骨组织工程支架材料,成骨细胞复合纳米支架用于骨缺损的修复,具有广阔的临床应用前景。 展开更多
关键词 成骨细胞 纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架 生物相容性 组织工程学
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冻融循环恢复土壤压实过程中大孔隙作用研究
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作者 贺亭峰 张慧清 +3 位作者 张冬梅 刘化涛 孔猛 丁启朔 《农业机械学报》 EI CAS CSCD 北大核心 2023年第10期340-347,共8页
农机压实土壤的问题很难避免,而农田压实土壤的结构恢复至关重要,冻融循环是恢复压实土壤的有效方法。为了研究冻融循环+大孔隙对恢复压实土壤的作用,通过室内试验重塑压实土壤及人工孔隙措施模拟大孔隙,在不同土壤含水率条件下设计不... 农机压实土壤的问题很难避免,而农田压实土壤的结构恢复至关重要,冻融循环是恢复压实土壤的有效方法。为了研究冻融循环+大孔隙对恢复压实土壤的作用,通过室内试验重塑压实土壤及人工孔隙措施模拟大孔隙,在不同土壤含水率条件下设计不同的冻融循环次数,利用温度传感器监测土壤温度波动,同时对比冻融循环前后压实土壤孔隙与团聚体参数的变化。结果表明,冻融循环过程中,有人工孔隙压实土壤在高、低两种含水率条件下,分别在第3次和第2次冻融循环时开始大幅温度波动,相同含水率条件下无人工孔隙的压实土壤温度大幅波动则出现在第7次和第4次冻融循环,经历多次冻融循环后,有人工孔隙压实土壤的团聚体平均尺度、结构系数等参数均优于无人工孔隙的压实土壤。人工孔隙可通过调节土壤温度波动改变冻融循环在土壤中的作用强度,加速压实土壤结构恢复进程,冻融循环+大孔隙策略是改善土壤孔隙及团聚体结构的有效措施。 展开更多
关键词 土壤压实 结构恢复 冻融循环 人工孔隙 土壤温度
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不同尺度裂缝对弹性波速度和各向异性影响的实验研究 被引量:16
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作者 丁拼搏 狄帮让 +3 位作者 魏建新 王丁 李向阳 尹志恒 《地球物理学报》 SCIE EI CAS CSCD 北大核心 2017年第4期1538-1546,共9页
裂缝广泛分布于地球介质中并且具有多尺度的特点,裂缝尺度对于油气勘探和开发有着重要的意义.本文制作了一组含不同长度裂缝的人工岩样,其中三块含裂缝岩样中的裂缝直径分别为2 mm、3 mm和4 mm,裂缝的厚度都约为0.06 mm,裂缝密度大致相... 裂缝广泛分布于地球介质中并且具有多尺度的特点,裂缝尺度对于油气勘探和开发有着重要的意义.本文制作了一组含不同长度裂缝的人工岩样,其中三块含裂缝岩样中的裂缝直径分别为2 mm、3 mm和4 mm,裂缝的厚度都约为0.06 mm,裂缝密度大致相同(分别为4.8%、4.86%和4.86%).在岩样含水的条件下测试不同方向上的纵横波速度,实验结果表明,虽然三块裂缝岩样中的裂缝密度大致相同,但是含不同直径裂缝岩样的纵横波速度存在差异.在各个方向上,含数量众多的小尺度裂缝的岩样中纵横波速度都明显低于含少量的大尺度裂缝的岩样中纵横波速度.尤其是对纵波速度和SV波速度,在不同尺度裂缝岩样中的差异更明显.在含数量多的小尺度裂缝的岩样中纵波各向异性和横波各向异性最高,而含少量的大尺度的裂缝的岩样中的纵波各向异性和横波各向异性较低.实验测量结果与Hudson理论模型预测结果进行了对比分析,结果发现Hudson理论考虑到了裂缝尺度对纵波速度和纵波各向异性的影响,但是忽略了其对横波速度和横波各向异性的影响. 展开更多
关键词 多尺度裂缝 各向异性 物理模型 岩石物理 孔隙人工岩心
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Characteristics of Soil Porosity and Changes of Soil Water Content in Eucalyptus Plantation 被引量:1
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作者 熊柳梅 黄金生 +7 位作者 曾艳 黄雁飞 陈桂芬 刘永贤 周柳强 谭宏伟 黄美福 黄玉溢 《Agricultural Science & Technology》 CAS 2015年第4期662-667,共6页
Objective] This study almed to investigate the characteristics of soiI total porosity (STP) and various factors affecting soiI water content (SWC) in eucalyptus pIantation (EP), thereby providing references for ... Objective] This study almed to investigate the characteristics of soiI total porosity (STP) and various factors affecting soiI water content (SWC) in eucalyptus pIantation (EP), thereby providing references for soiI water utiIization in eucalyptus pIanting in the red soiI hiI y region of South China. [Method] In the same cIimatic region, soiI sampIes were coI ected from surface soiI Iayer (A), iI uvial horizon (B) and parent material horizon (C) in the upper sIope, middIe sIope and Iower sIope of eucalyptus pIantation, native forest and pine forest, respectiveIy, to determine the soiI porosity and soiI water content and analyze changes and various infIuencing factors of soiI water content in horizontal and vertical direction. [Result] Average soiI porosity in eucalyptus pIantation, native forest and pine forest was 45.9%, 41.4%and 55.3%, respectiveIy; soiI water content in these three forest stands was 13.3%, 13.4% and 15.5%, respectiveIy. In addition, soiI water content in these three forest stands exhibited no significant differences (P&gt;0.05) among different soiI profiIes and sIope positions, but soiI water content in surface soiI Iayer varied significantIy (P&lt;0.05) among different forest stands; in the horizontal direction, soiI water content exerted an extremeIy significant positive correIation with total coverage. [Conclusion] Total coverage of canopy Iayer, herb and Iitter Iayer is one of the most critical fac-tors affecting the changes of soiI water content in surface soiI Iayer of forest stands. 展开更多
关键词 Eucalyptus plantatlon Soil porosity Changes of soil water content Red soli Hilly reglon South China
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Influence of Organic Matter Content on Hydro-Structural Properties of Constructed Technosols 被引量:1
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作者 Maha DEEB Michel GRIMALDI +3 位作者 Thomas Z.LERCH Anne PANDO Pascal PODWOJEWSKI Manuel BLOUIN 《Pedosphere》 SCIE CAS CSCD 2016年第4期486-498,共13页
Constructed Technosols may be an alternative for creating urban green spaces. However, the hydro-structural properties emer- ging from the assembly of artefacts have never been documented. The soil shrinkage curve (... Constructed Technosols may be an alternative for creating urban green spaces. However, the hydro-structural properties emer- ging from the assembly of artefacts have never been documented. The soil shrinkage curve (SSC) could provide relevant structural information about constructed Technosols, such as the water holding capacity of each pore system (macropores and micropores). The objectives of this study were (i) to evaluate the SSC and water retention curve (WRC) to describe the structure of constructed Tech- nosols and (ii) to understand the influence of organic matter content on soil hydro-structural properties. In this study, Technosols were obtained by mixing green waste compost (GWC) with the material excavated from deep horizons of soil (EDH). The CWC was mixed with EDH in six different volumetric percentages from 0% to 50% (GWC/total). The GWC and EDH exhibited highly divergent hydro-structural properties: the SSC was hyperbolic for GWC and sigmoid for EDH. All six mixture treatments (0%, 10%, 20%, 30%, 40% and 50% GWC) exhibited the classical sigmoid shape, revealing two embedded levels of pore systems. The 20% GWC treatment was hydro-structurally similar to the 30% and 40% GWC treatments; so, a large quantity of expansive GWC is unnecessary. The relation with the GWC percentage was a second-degree equation for volumetric available water in micropores, but was linear for volumetric available water in macropores and total volumetric available water. Total volumetric available water in the 50% GWC treatment was twice as high as that in the 0% GWC treatment. By combining SSCs and WRCs, increasing the GWC percentage increased water holding capacity by decreasing the maximum equivalent size of water-saturated micropores at the shrinkage limit and increasing the maximum equivalent size of water-saturated macropores, resulting in an increased range of pore diameter able to retain available water. 展开更多
关键词 available water soil shrinkage curve soil water content water holding capacity water retention curve
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