[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extru...[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.展开更多
Mating behavior and sexual selection in relation to morphometric traits in a polygamous beetle, Glenea cantor （F.） （Coleoptera： Cerambycidae）, were investigated. Upon encounter, a male approached a female, mounte...Mating behavior and sexual selection in relation to morphometric traits in a polygamous beetle, Glenea cantor （F.） （Coleoptera： Cerambycidae）, were investigated. Upon encounter, a male approached a female, mounted her, grasped her terminal abdomen with his hind tarsi, and attempted to mate. Successful mating lasted about 3.5 h. Although all traits measured in females and half of traits in males were significantly correlated with mating success, the primary selection on virgin females was the genital trait, thebursa copulatrix length, and that on males was the body length and hind tarsal length. Longer bursa copulatrix accommodated a larger ejaculate, suggesting that this female trait benefits the male that first mates with the female in terms of in- creasing ejaculate size to beat subsequent males in sperm competition. Under a female-biased sex ratio, more than 20% of mat- ings failed within 20s after the male genitalia had been inserted into hers, suggesting that males assess genital features of the re- ：male before insemination and undertake cryptic male mate choice. Larger males were more capable of grasping females and achieving mating. During the premating struggle the male almost always used his hind tarsi to lift the female terminal abdomen to the position for his genitalia to insert, and as a result, males with longer hind tarsi achieved higher mating success [Current Zoolo- gy 59 （2）： 257-264, 2013].展开更多
文摘[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.
文摘Mating behavior and sexual selection in relation to morphometric traits in a polygamous beetle, Glenea cantor （F.） （Coleoptera： Cerambycidae）, were investigated. Upon encounter, a male approached a female, mounted her, grasped her terminal abdomen with his hind tarsi, and attempted to mate. Successful mating lasted about 3.5 h. Although all traits measured in females and half of traits in males were significantly correlated with mating success, the primary selection on virgin females was the genital trait, thebursa copulatrix length, and that on males was the body length and hind tarsal length. Longer bursa copulatrix accommodated a larger ejaculate, suggesting that this female trait benefits the male that first mates with the female in terms of in- creasing ejaculate size to beat subsequent males in sperm competition. Under a female-biased sex ratio, more than 20% of mat- ings failed within 20s after the male genitalia had been inserted into hers, suggesting that males assess genital features of the re- ：male before insemination and undertake cryptic male mate choice. Larger males were more capable of grasping females and achieving mating. During the premating struggle the male almost always used his hind tarsi to lift the female terminal abdomen to the position for his genitalia to insert, and as a result, males with longer hind tarsi achieved higher mating success [Current Zoolo- gy 59 （2）： 257-264, 2013].
