目的对房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障患者的分析。方法选择我院2013年1月至2014年7月收治的行房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障的126例患者作为研究对象,进行回顾性分析...目的对房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障患者的分析。方法选择我院2013年1月至2014年7月收治的行房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障的126例患者作为研究对象,进行回顾性分析,对手术前后的前房深度、眼压、视力进行观察和记录,进行1年的随访。结果所有患者术后前房深度较术前均有明显加深;125例患者术后眼压较术前明显降低,均小于20 mm Hg;123例患者术后视力得以提高。结论房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障患者的临床疗效较好,可以有效地使眼内压降低、房角加深、视力提高,值得在临床上进行推广。展开更多
AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by ...AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin unit.RESULTS A complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with Bam H I digestion. The GFPCre ERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCre ERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers. CONCLUSION The construct of Gpm6 a^(GFPCreERT2) or Reelin^(GFPCreERT2) was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.展开更多
Crop-livestock integration(CLI)is a significant practice for livestock grazing systems in alpine rangelands.It offers the potential to achieve sustainable crop and livestock production.However,the separate crop and li...Crop-livestock integration(CLI)is a significant practice for livestock grazing systems in alpine rangelands.It offers the potential to achieve sustainable crop and livestock production.However,the separate crop and livestock systems that exist today have led to issues of intensive agriculture,rangeland degradation and forage shortage in the Tibetan Plateau.Developing crop-livestock integration through sown pastures can be an effective way to lift pasture productivity and improve livestock production.Thus,to explore the potential for integrating crop and livestock production in alpine grazing systems,an assessment of potential forage and livestock production using multiple datasets was carried out in Burang County,China.Results showed the marginal land potentially available for sown pastures was about 560 ha,located mostly in the Burang township of the Karnali basin.Accumulated temperature was the dominant limiting factor for establishing sown pastures,therefore cold tolerance of forage species and growth period should be taken into consideration.Furthermore,the number of livestock decreased during the period 2012–2016;yet often,the number of livestock in rangeland landscape was greater than that in agro-pastoral landscape.The average number of livestock was about 110000 standard sheep units(SU)in the study area,but forage from sown pastures and crop residues could potentially feed about 11000 SU,accounting for 50%of the livestock population in the Karnali basin.We found that integrating crop and forage production could fill feed gaps for grazing systems,particularly in the agro-pastoral landscape of the Karnali basin.The results of this study provide scientific support to guide future forage production and to promote further crop and livestock integration in Burang County.展开更多
文摘目的对房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障患者的分析。方法选择我院2013年1月至2014年7月收治的行房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障的126例患者作为研究对象,进行回顾性分析,对手术前后的前房深度、眼压、视力进行观察和记录,进行1年的随访。结果所有患者术后前房深度较术前均有明显加深;125例患者术后眼压较术前明显降低,均小于20 mm Hg;123例患者术后视力得以提高。结论房角分离术结合超声乳化人工晶体植入治疗闭角型青光眼合并白内障患者的临床疗效较好,可以有效地使眼内压降低、房角加深、视力提高,值得在临床上进行推广。
基金Supported by National Natural Science Foundation of China,No.81300349 and No.81270532the Beijing Natural Science Foundation,No.7144216+3 种基金the Beijing Nova Program,No.Z131107000413016the Project of Science and Technology Activities of Preferred Overseas Personnel of Beijing(2014)the Project of Cultivation of High Level Medical Technical Personnel in the Health System of Beijing,No.2014-3-090 and No.2013-3-071Beijing Municipal Institute of public medical research development and reform pilot project,No.2016-2
文摘AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin unit.RESULTS A complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with Bam H I digestion. The GFPCre ERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCre ERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers. CONCLUSION The construct of Gpm6 a^(GFPCreERT2) or Reelin^(GFPCreERT2) was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.
基金The National Key Research and Development Program of China(2016YFC0502001,2016YFC0501803)Kailash Sacred Landscape Conservation and Development Initiative from ICIMOD
文摘Crop-livestock integration(CLI)is a significant practice for livestock grazing systems in alpine rangelands.It offers the potential to achieve sustainable crop and livestock production.However,the separate crop and livestock systems that exist today have led to issues of intensive agriculture,rangeland degradation and forage shortage in the Tibetan Plateau.Developing crop-livestock integration through sown pastures can be an effective way to lift pasture productivity and improve livestock production.Thus,to explore the potential for integrating crop and livestock production in alpine grazing systems,an assessment of potential forage and livestock production using multiple datasets was carried out in Burang County,China.Results showed the marginal land potentially available for sown pastures was about 560 ha,located mostly in the Burang township of the Karnali basin.Accumulated temperature was the dominant limiting factor for establishing sown pastures,therefore cold tolerance of forage species and growth period should be taken into consideration.Furthermore,the number of livestock decreased during the period 2012–2016;yet often,the number of livestock in rangeland landscape was greater than that in agro-pastoral landscape.The average number of livestock was about 110000 standard sheep units(SU)in the study area,but forage from sown pastures and crop residues could potentially feed about 11000 SU,accounting for 50%of the livestock population in the Karnali basin.We found that integrating crop and forage production could fill feed gaps for grazing systems,particularly in the agro-pastoral landscape of the Karnali basin.The results of this study provide scientific support to guide future forage production and to promote further crop and livestock integration in Burang County.
